Rapid Plasma Reagin Card Test for Syphilis and Other Treponematoses

Portnoy, Joseph; Brewer, John H.; Harris, Ad

doi:10.2307/4591589

Cited by 76 publications

(22 citation statements)

References 3 publications

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“…These lipids are thought to be derived from the host and incorporated into the membrane of the metabolically limited T. pallidum (105), producing a configuration that is antigenic. Decades later, the antilipoidal antibody VDRL and rapid plasma reagin (RPR) tests were developed (132,237). Automation, antigen stability, the ability to use plasma rather than serum, and macroscopic observation (199) make the RPR test more conducive for use in clinical laboratories.…”

Section: Antibodies In Diagnostic Testingmentioning

confidence: 99%

Biological Basis for Syphilis

2006

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SUMMARY Syphilis is a chronic sexually transmitted disease caused by Treponema pallidum subsp. pallidum. Clinical manifestations separate the disease into stages; late stages of disease are now uncommon compared to the preantibiotic era. T. pallidum has an unusually small genome and lacks genes that encode many metabolic functions and classical virulence factors. The organism is extremely sensitive to environmental conditions and has not been continuously cultivated in vitro. Nonetheless, T. pallidum is highly infectious and survives for decades in the untreated host. Early syphilis lesions result from the host's immune response to the treponemes. Bacterial clearance and resolution of early lesions results from a delayed hypersensitivity response, although some organisms escape to cause persistent infection. One factor contributing to T. pallidum's chronicity is the paucity of integral outer membrane proteins, rendering intact organisms virtually invisible to the immune system. Antigenic variation of TprK, a putative surface-exposed protein, is likely to contribute to immune evasion. T. pallidum remains exquisitely sensitive to penicillin, but macrolide resistance has recently been identified in a number of geographic regions. The development of a syphilis vaccine, thus far elusive, would have a significant positive impact on global health.

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Section: Antibodies In Diagnostic Testingmentioning

confidence: 99%

Biological Basis for Syphilis

2006

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“…The Rapid Plasm Reagin (RPR) "teardrop" card assay for fingerprick blood samples developed in 60' was the first serological test for screening of syphilis, which could be applied in field conditions without the conventional laboratory equipments 8 . DYCK et al (1993) 1 evaluated the performance of the RPR "teardrop" test with plasma obtained from finger-prick of 466 pregnant women, and the authors observed lower diagnostic features of the RPR "teardrop" test in comparison to the classical RPR performed with serum.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

Sato

Melo

Zerbini

et al. 2003

Rev. Inst. Med. trop. S. Paulo

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SUMMARYA rapid test based on an immunochromatography assay -Determine Syphilis TP (Abbott Lab.) for detecting specific antibodies to Treponema pallidum was evaluated against serum samples from patients with clinical, epidemiological and serological diagnosis of syphilis, patients with sexually transmitted disease other than syphilis, and individuals with negative serology for syphilis. The Determine test presented the sensitivity of 93.6%, specificity of 92.5%, and positive predictive value and negative predictive value of 95.2% and 93.7%, respectively. One serum sample from patient with recent latent syphilis showed a prozone reaction. Determine TM is a rapid assay, highly specific and easy to perform. This technique obviates the need of equipment and its diagnostic features demonstrate that it may be applicable as an alternative assay for syphilis screening under some emergency conditions or for patients living in remote localities.

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“…RECENTLY reported modification of the rapid plasma reagin (RPR) card test (1) (2) The comparison of the quantitative RPR (circle) card and the VDRL slide tests on 377 serums, considering agreement to be identical titers or within plus or minus one dilution, showed a 75.9 percent agreement between the two tests. Disagreement in titer occurred in 24.1 percent of these specimens and in the majority of instances where differences in titer occurred, the quantitative RPR (circle) card test was two or more dilutions higher.…”

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confidence: 99%