Evaluation of Rapid Plasma Reagin (Circle) Card Test

Falcone, Virginia H.; Stout, G; Moore, Mary B.

doi:10.2307/4592169

Cited by 18 publications

(18 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The comparison of the quantitative RPR card and VDRL slide tests (Table II) showed that the former was more sensitive with the majority of sera.This finding was similar to that of Falcone, Stout, and Moore (1964). In the present study difficulty was sometimes found in reading the end-point in the RPR card test titration, as there was only a gradual change in the appearance of the flocculation through the dilutions.…”

Section: Discussionsupporting

confidence: 89%

Comparison of the rapid plasma reagin card test and other screening tests for treponemal disease in patients attending a venereal disease clinic.

Scrimgeour¹,

Rodin²

1973

Sexually Transmitted Infections

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 89%

Comparison of the rapid plasma reagin card test and other screening tests for treponemal disease in patients attending a venereal disease clinic.

Scrimgeour¹,

Rodin²

1973

Sexually Transmitted Infections

View full text Add to dashboard Cite

“…1,5 The reasons for this are the limited sensitivity and disease specificity of the standard non-treponemal tests. The sensitivity of the macroscopic card tests, the modified version of the original rapid plasma reagin (RPR) test, the RPR circle card test 3,6 and the Toluidine red unheated serum test (TRUST), 1 cardiolipin, cholesterol and lecithin, 1,2,5 varies according to the stage of syphilis, 7 and might be negative in late latent syphilis. 7,8 With regard to disease specificity, reactivity in the non-treponemal tests may be unrelated to Treponema pallidum infection, a condition called a biological false-positive (BFP) syphilis test.…”

Section: Introductionmentioning

confidence: 99%

Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera

et al. 2005

View full text Add to dashboard Cite

This retrospective study on syphilis screening at the sexually transmitted infection (STI) unit of a University Department emphasizes the necessity of a treponemal-specific test as the appropriate screening test. The Venereal Disease Research Laboratory (VDRL) test for syphilis screening may, under certain circumstances, yield positive results in patients not infected with Treponema pallidum, a phenomenon referred to as biological false-positive (BFP) VDRL test. The aim of this study was to determine the frequency of BFP tests in a large sample of sera. In this retrospective study, we analysed the results of parallel VDRL and T. pallidum haemagglutination (TPHA) testing of a total of 514,940 blood samples obtained from patients at the Vienna General Hospital between January 1988 and November 1999. Patients' sera with incomplete data on stage and sex and duplicate sera were excluded, leaving 300,000 sera for analysis. The seroprevalence for syphilis was 1.77% (n = 5320), as determined by a positive TPHA test. It was significantly higher in male than in female patients (2.03% versus 1.58%, P<0.001). Of the patients reactive in the TPHA test, 3257 (61.2%) were negative in the VDRL. With regard to reactivity in VDRL testing, 2799 patients (0.92%) of the study population were positive, of whom 736 (26%) were biological false positive. BFP reactivity was found in 0.24% of all patients and was significantly higher in women than in men (0.27% versus 0.20%, P<0.001) and in patients over 60 years of age (0.34%) as compared with those under 60 (0.25%, P<0.001). This proportion might be even higher, as reactivity in the VDRL at 1:0 and 1:2 dilutions without a positive treponemal test was not reported. The subgroup of HIV-positive patients (n = 1415) revealed a 10-fold higher rate of BFP tests (2.1% versus 0.24), an effect being statistically significant. In a low syphilis prevalence population, BFP reactions comprise a high proportion of all VDRL reactors. Therefore, the use of the VDRL as a screening procedure is challenged.

show abstract

“…In the RPR test, the antigen suspension incorporates charcoal particles to enhance flocculation (9), while in the toluidine red unheatedserum test (TRUST), the carbon particles were replaced with toluidine red particles (10).…”

Section: Serologic Testsmentioning

confidence: 99%

Recent Trends in the Serologic Diagnosis of Syphilis

Morshed

Singh

2014

Clin. Vaccine Immunol.

163

156

View full text Add to dashboard Cite

bComplexities in the diagnosis of syphilis continue to challenge clinicians. While direct tests (e.g., microscopy or PCR) are helpful in early syphilis, the mainstay of diagnosis remains serologic tests. The traditional algorithm using a nontreponemal test (NTT) followed by a treponemal test (TT) remains the standard in many parts of the world. More recently, the ability to automate the TT has led to the increasingly widespread use of reverse algorithms using treponemal enzyme immunoassays (EIAs). Rapid, point-of-care TTs are in widespread use in developing countries because of low cost, ease of use, and reasonable performance. However, none of the current diagnostic algorithms are able to distinguish current from previously treated infections. In addition, the reversal of traditional syphilis algorithms has led to uncertainty in the clinical management of patients. The interpretation of syphilis tests is further complicated by the lack of a reliable gold standard for syphilis diagnostics, and the newer tests can result in false-positive reactions similar to those seen with older tests. Little progress has been made in the area of serologic diagnostics for congenital syphilis, which requires assessment of maternal treatment and serologic response as well as clinical and laboratory investigation of the neonate for appropriate management. The diagnosis of neurosyphilis continues to require the collection of cerebrospinal fluid for a combination of NTT and TT, and, while newer treponemal EIAs look promising, more studies are needed to confirm their utility. This article reviews current tests and discusses current controversies in syphilis diagnosis, with a focus on serologic tests.

show abstract

Evaluation of Rapid Plasma Reagin (Circle) Card Test

Cited by 18 publications

References 3 publications

Comparison of the rapid plasma reagin card test and other screening tests for treponemal disease in patients attending a venereal disease clinic.

Comparison of the rapid plasma reagin card test and other screening tests for treponemal disease in patients attending a venereal disease clinic.

Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera

Recent Trends in the Serologic Diagnosis of Syphilis

Contact Info

Product

Resources

About