Rapid PCR detection of Salmonella in horse faecal samples

Amavisit, P.; Browning, Glenn F.; Lightfoot, Diane; Church, S; Anderson, GA; Whithear, Kevin G.; Markham, Philip F.

doi:10.1016/s0378-1135(00)00340-0

Cited by 53 publications

(34 citation statements)

References 23 publications

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“…It is estimated that the infections due to Salmonella cause 1.4 million cases annually and result in economic losses between 500 million to 2.3 billion dollars in the US (12,13). Poultry products can be the main source of human diseases caused by the pathogenic bacteria of Salmonella (14). Polymerase chain reaction has been reported widely for detection of Salmonella spp.…”

Section: Discussionmentioning

confidence: 99%

Development of a Polymerase Chain Reaction-Temporal Temperature Gradient Gel Electrophoresis Assay for Identification of Salmonella enterica Subspecies enterica Using a Hypothetical Non-specific Endonucleas S. entericae Gene Sequence

Besharati

Bahrami

Mashreghi

et al. 2017

Jundishapur J Microbiol

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Background: Salmonella is one of the major agents of food-borne diseases that cause severe illness in humans. The conventional detection methods of these bacteria are time-consuming with low sensitivity and specificity, which limit their applications. Therefore, developing more rapid and accurate methods is urgently required in food safety programs. Objectives: In this study for the first time, polymerase chain reaction-Temporal temperature gradient gel electrophoresis (TTGE) was optimized for the identification of Salmonella enterica subspecies enterica serovars in processed food samples using a single-copy sequence. Methods: DNA was isolated from pure cultures of Salmonella and non-Salmonella strains. The single copy target sequence was selected and amplified by employing the polymerase chain reaction (PCR) with specific primers, designed in this study, and their specificity and sensitivity were determined. The TTGE parameters, especially the temperature gradient and the time of reaction, were optimized and then this method was applied to investigate spiked food samples.

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Section: Discussionmentioning

confidence: 99%

Development of a Polymerase Chain Reaction-Temporal Temperature Gradient Gel Electrophoresis Assay for Identification of Salmonella enterica Subspecies enterica Using a Hypothetical Non-specific Endonucleas S. entericae Gene Sequence

Besharati

Bahrami

Mashreghi

et al. 2017

Jundishapur J Microbiol

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“…There are numerous commercial kits available to extract DNA from feces; some that have been specifically designed for stool samples include the QIAamp® DNA stool minikit (Qiagen, Germany), the MagneSil™ KF Genomic System (Promega) and the MagaZorb® DNA Mini Prep kit (Promega). Although these kits yield DNA that gives positive results for amplification, they do not perform as effectively with fecal samples when compared with extractions from samples of pure bacterial culture [31][32][33]. One other key challenge for molecular-based diagnostics is the standardization of assays between laboratories.…”

Section: Potential Problems In Developing Molecular Detection Methodsmentioning

confidence: 99%

Development of rapid, automated diagnostics for infectious disease: advances and challenges

Ince

McNally²

2009

Expert Review of Medical Devices

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The last 2 years has seen an exponential rise in the amount of research funding made available for the development of rapid diagnostic devices for infectious agents of medical importance. This review reports on several such projects. These highlight the development of fully automated devices for rapid diagnostics, ranging from fully automated real-time PCR-based detection methods to fully automated PCR-and array-based machines for the detection and typing of influenza. This review will also highlight the importance of refocusing work on classical immunoassay techniques, showing how biosensor-based immunoassays can greatly enhance existing assays and at a much reduced cost to molecular-based methods.

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“…The isolates identified as Salmonella spp. from the Hektoen and XLT4 agar plates were further characterised by PCR (AMAVISIT et al, 2001) and serotyped at the Salmonella Reference Laboratory at the Instituto Oswaldo Cruz/Fundação Oswaldo Cruz (Rio de Janeiro, RJ, Brazil). E. coli was recovered from MacConkey plates, and multiplex PCR for E. coli virulence factor genes (FRANCK et al, 1998) Calf 4 (40 days old) exhibited neuropathy, as characterised by lateral recumbency, severe depression, opisthotonos, paddling, a rectal temperature of 37.5°C, bradypnea, and bradycardia.…”

Section: Case Reportmentioning

confidence: 99%

Systemic and enteric salmonellosis in calves

et al. 2015

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This report describes the clinical manifestation of different Salmonella serovars in dairy calves. S. typhimurium was identified in faecal samples of a calf with rectal prolapse and in faecal samples and mesenteric lymph nodes of a calf with necrosis at the tip of the tail. S. agona was identified in faecal samples of a calf with diarrhoea but no other clinical manifestation, and S. dublin was observed in faecal and organ samples from a calf with neurological symptoms. Assays to differentiate between the main enteric pathogens (Enteropathogenic E. coli, rotavirus and coronavirus) were performed and were negative. Due to the negative impact of salmonellosis and occurrence of different serovars and clinical manifestations in calves, the correct diagnosis is important to identify control and prophylactic measures in a dairy herd. Key words: Salmonellosis, calves, diagnosis, serovars ResumoEste relato de caso descreve a manifestação clínica de diferentes sorovares de Salmonella em bezerros leiteiros. A S. typhimurium foi identificado em amostras fecais de um bezerro com prolapso rectal e em amostras fecais e linfonodos mesentéricos de um bezerro com necrose na ponta da cauda. Já a S. agona foi identificada em amostras de fezes de um bezerro com diarréia, mas sem nenhuma outra manifestação clínica, e a S. dublin foi observada em amostras de fezes e de órgãos a partir de um bezerro com sinais clínicos neurológicos. Diagnóstico diferencial para os principais patógenos entéricos (E. coli enteropatogênica, rotavirus e coronavirus) foram realizados e foram negativos. Devido ao impacto negativo da salmonelose e a ocorrência de diferentes sorotipos e manifestações clínicas em bezerros, o diagnóstico correto é importante para identificar formas de controle e medidas profiláticas no rebanho leiteiro.

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Rapid PCR detection of Salmonella in horse faecal samples

Cited by 53 publications

References 23 publications

Development of a Polymerase Chain Reaction-Temporal Temperature Gradient Gel Electrophoresis Assay for Identification of Salmonella enterica Subspecies enterica Using a Hypothetical Non-specific Endonucleas S. entericae Gene Sequence

Development of a Polymerase Chain Reaction-Temporal Temperature Gradient Gel Electrophoresis Assay for Identification of Salmonella enterica Subspecies enterica Using a Hypothetical Non-specific Endonucleas S. entericae Gene Sequence

Development of rapid, automated diagnostics for infectious disease: advances and challenges

Systemic and enteric salmonellosis in calves

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