Rapid pathogen detection tools in seafood safety

Bavisetty, Sri Charan Bindu; Vu, Hue Thi Kim; Benjakul, Soottawat; Vongkamjan, Kitiya

doi:10.1016/j.cofs.2018.05.013

Cited by 23 publications

(13 citation statements)

References 40 publications

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“…La capacidad que posee este método asignando correctamente una muestra positiva en el análisis presuntivo corresponde a un 85%. Cabe destacar que los métodos de tipo molecular se ven afectados por diversas características propias de la matriz, como la presencia de inhibidores, grasa Es importante enfatizar que la PCR, así como el inmunoensayo visual, complementa pero no reemplaza las técnicas microbiológicas tradicionales, debido a que los resultados positivos presuntivos deben confirmarse por el método convencional 15 .…”

Section: Discussionunclassified

Comparación de dos métodos rápidos para la detección de Salmonella spp. con un método convencional

Burgos

Alberti²

2019

ALERTA

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Introducción. Uno de los microorganismos relacionados a las enfermedades transmitidas por alimentos a nivel mundial es Salmonella spp. El control sanitario de este patógeno es una de las principales preocupaciones en salud pública. La detección de Salmonella spp se realiza principalmente por métodos convencionales de cultivo y métodos rápidos automatizados, la diferencia entre ellos se centra en el tiempo de emisión de resultados. Los métodos automatizados son más rápido que los convencionales, tener los resultados en menos tiempo, es importante en salud pública. Objetivos. Comparar el desempeño de dos métodos rápidos para la detección de Salmonella spp, el método de reacción de la cadena de la polimerasa Assurance GDS System, y el inmunoensayo visual VIA TECRA, con el método convencional para la detección de Salmonella spp ISO 6579:2002, IDT, considerado de referencia internacional. Metodología. Se analizaron 96 muestras de carne fresca de pollo, proveniente de supermercados del área metropolitana de San Salvador. Las muestras fueron analizadas simultáneamente por los tres métodos en estudio. Se realizaron los cálculos de sensibilidad, especificidad, tasa de falsos positivos, tasa de falsos negativos y Chi cuadrado descrito por McNemar. Resultados. El método alternativo inmunoensayo, obtuvo los mejores resultados al compararlo los resultados del método convencional, se obtuvo un 88% de exactitud relativa, 88% de sensibilidad, un 86% de especificidad, 14% de tasa de falsos positivos y un 12% de tasa de falsos negativos, mientras que al realizar la comparación con el metodo Assurance GDS System se obtuvo un 85% de sensibilidad, un 81% de especificidad, 19% de tasa de falsos positivos y un 15% de tasa de falsos negativos. Al evaluar los datos discordantes y aplicar el test de Chi cuadrado, se determinó que no existe diferencia significativa entre los métodos evaluados (P≤0.05). Conclusiones. El método alternativo inmunoensayo visual, obtuvo los mejores resultados contra el método convencional.

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Section: Discussionunclassified

Comparación de dos métodos rápidos para la detección de Salmonella spp. con un método convencional

Burgos

Alberti²

2019

ALERTA

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“…ELISA is able to detect targets quicker but compared to other methods, it has a lower specificity and needs multiple processing steps including rinsing and reagent addition steps, which require few hours to complete the tests [ 22 ]. Similar to ELISA, qPCR is tedious and requires numerous operation steps (i.e., nucleic acid extraction, amplification and detection) [ 23 ]. Collectively, the abovementioned conventional detection methods are high-cost and laborious, making them less applicable in resource-poor settings with limited accessibility to well-established laboratories.…”

Section: Importance Of Rapid Point-of-care Testing For Food Safetymentioning

confidence: 99%

Emerging Point-of-care Technologies for Food Safety Analysis

Choi

Yong

Choi

et al. 2019

Sensors

126

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Food safety issues have recently attracted public concern. The deleterious effects of compromised food safety on health have rendered food safety analysis an approach of paramount importance. While conventional techniques such as high-performance liquid chromatography and mass spectrometry have traditionally been utilized for the detection of food contaminants, they are relatively expensive, time-consuming and labor intensive, impeding their use for point-of-care (POC) applications. In addition, accessibility of these tests is limited in developing countries where food-related illnesses are prevalent. There is, therefore, an urgent need to develop simple and robust diagnostic POC devices. POC devices, including paper- and chip-based devices, are typically rapid, cost-effective and user-friendly, offering a tremendous potential for rapid food safety analysis at POC settings. Herein, we discuss the most recent advances in the development of emerging POC devices for food safety analysis. We first provide an overview of common food safety issues and the existing techniques for detecting food contaminants such as foodborne pathogens, chemicals, allergens, and toxins. The importance of rapid food safety analysis along with the beneficial use of miniaturized POC devices are subsequently reviewed. Finally, the existing challenges and future perspectives of developing the miniaturized POC devices for food safety monitoring are briefly discussed.

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“…Foodborne illness is one of the significant public health problems worldwide. Therefore, microbiological safety of food has become an important concern for consumers, various industries, and regulatory agencies [1]. There are many different groups of microorganisms in food.…”

Section: Introductionmentioning

confidence: 99%

“…Rapid detection methods of foodborne pathogens can be categorized into nucleic acid-, antigen-antibody-based, biosensorbased, and bacteriophage-based methods. Biosensor-based methods have been increasingly gaining popularity owing to their characteristic feature of rapid detection of foodborne pathogens [1]. In addition to the rapid results, online biosensor technology offers the food industry a tool for internal process control to fulfil the high standard of quality control [7].…”

Section: Introductionmentioning

confidence: 99%

Biosensors: A Novel Approach to Detect Food-borne Pathogens.

Sentürk¹,

Aktop²,

Şanlibaba³

et al. 2018

Appli Microbiol Open Access

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Foodborne pathogens affect human health negatively and are known to cause economic losses. Therefore, quick detection of foodborne pathogens and the implementation of measures to ensure their inactivation are of immense significance. Immunological, molecular, and cultural methods are frequently used in the detection of foodborne pathogens. High cost, prolonged analysis times, and the necessity of specialized personnel are some of the disadvantages of these methods. Biosensors are known as analytical devices. The use of biosensors is considered a new approach to quickly detect foodborne pathogens and their toxins. Biosensors, which are capable of converting biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. Different types of biosensor are being employed for detection of pathogenic bacteria. Biosensors are sensitive, fast, economical, reliable, and portable devices, and are used in many fields such as food safety, medicine, pharmacy, measurement of environmental pollution, and the military defense. Electrochemical and optical biosensors and piezoelectric immunosensors are among the most frequently used biosensors in the detection of foodborne pathogens. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in food industry are summarized.

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Rapid pathogen detection tools in seafood safety

Cited by 23 publications

References 40 publications

Comparación de dos métodos rápidos para la detección de Salmonella spp. con un método convencional

Comparación de dos métodos rápidos para la detección de Salmonella spp. con un método convencional

Emerging Point-of-care Technologies for Food Safety Analysis

Biosensors: A Novel Approach to Detect Food-borne Pathogens.

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