Rapid neural differentiation of human cord blood-derived mesenchymal stem cells

Jeong, Ju Ah; Gang, Eun Ji; Hong, Seung Hyun; Hwang, Soo Han; Kim, Seong Whan; Yang, In; Ahn, Chiyoung; Han, Hoon; Kim, Hoeon

doi:10.1097/01.wnr.0000134846.79002.5c

Cited by 83 publications

(50 citation statements)

References 11 publications

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“…Six full-term UCB units, each containing about 80 mm of blood, were processed as previously described [24][25][26]. For the isolation of MSCs, MNCs were plated at a density of 1 × 10 6 cells per cm 2 and allowed to adhere to culture flasks for 5 days.…”

Section: Cell Growth and Total Rna Isolationmentioning

confidence: 99%

“…UCB-derived cells were proven to be more advantageous in cell procurement, storage, and transplantation than their bone marrow (BM) counterpart and therefore better suited in tissue engineering and development of cell-based therapeutics. A number of reports from different laboratories [19][20][21][22][23], including ours [24][25][26], indicate that UCB-derived MSCs are highly similar to the cells of BM origin with respect to cell characteristics and multilineage differentiation potential. Therefore, this study may lead us to reveal the molecular signature that is specific to human MSCs but independent of their origins, and it will assist further studies on molecular mechanisms controlling various core stem cell properties.…”

Section: Introductionmentioning

confidence: 99%

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Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

Jeong¹,

Hong²,

Gang³

et al. 2005

STEM CELLS

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104

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Mesenchymal stem cells (MSCs) retain both self-renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the stemness, remain largely unknown. Using high-density oligonucleotide microarrays, we obtained the differential gene expression profile between a fraction of mononuclear cells of human umbilical cord blood (UCB) and its MSC subpopulation. Of particular interest was a subset of 47 genes preferentially expressed at 50-fold or higher in MSCs, which could be regarded as a molecular foundation of human MSCs. This subset contains numerous genes encoding collagens, other extracellular matrix or related proteins, cytokines or growth factors, and cytoskeleton-associated proteins but very few genes for membrane and nuclear proteins. In addition, a direct comparison of this microarray-generated transcriptome with the published serial analysis of gene expression data suggests that a molecular context of UCB-derived MSCs is more or less similar to that of bone marrow-derived cells. Altogether, our results will provide a basis for studies on molecular mechanisms controlling core properties of human MSCs. Stem Cells 2005;23:584-593

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Section: Cell Growth and Total Rna Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

Jeong¹,

Hong²,

Gang³

et al. 2005

STEM CELLS

Self Cite

104

View full text Add to dashboard Cite

show abstract

“…Some reports have shown that the umbilical cord blood contains cells that may express neuronal markers (Buzanska, Machaj, Zablocka, Pojda, & DomanskaJanik, 2002; Sanchez-Ramos et al, 2001). The umbilical cord is also a rich source of hematopoietic and mesenchymal stem cells (Wang et al, 2004) and has compelling potential for therapeutic use in neurological diseases due to the ability of these cells to differentiate into neural lineages (Buzanska et al, 2002;Jeong et al, 2004;Li et al, 2004;McGuckin, Forraz, Allouard, & Pettengell, 2004;Rogers & Casper, 2004;Sanchez-Ramos et al, 2001).…”

Section: Umbilical Cord Bloodmentioning

confidence: 99%

Stem cell grafts as therapeutic tools for central nervous system disorders.

Oliveira

Haeser

Pranke

2008

Psychology & Neuroscience

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The last few years have seen a growing interest in stem cell research. The media has helped to keep attention to the subject making it as popular as therapeutic cloning. A number of studies highlighted the importance of stem cells as prospective therapy for neurodegenerative conditions. The possibility of successful treatment for such pathologies using stem cells has raised enormous hope. Whilst in some countries biotechnologists have realised the commercial potential of the field, human stem cell research and its clinical applications have constantly been subject of ethical and legal debate. The present review brings an overview of recent aspects of stem cell research for central nervous system disorders, with special attention to Parkinson's and Alzheimer's diseases, cerebral ischemic insult and spinal cord injury. Furthermore, the ethical implications regarding the use of embryonic stem cells as a fundamentally more promising cell type are discussed.

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“…Therefore, HUCB is of significant value as good source of MSCs. Indeed, it was reported that HUCB-derived MSC (HMSCs) have multilineage differentiation activity rather than BM-derived MSC (20). Recently, numerous studies have ascribed not only potent tissue regeneration and repair but also anti-inflamatory effects of HMSCs in various diseases such as kidney disease, lung injury, hypoxia-induced brain injury, and liver cirrhosis (21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Human umbilical cord blood-derived mesenchymal stem cells improve glucose homeostasis in rats with liver cirrhosis

Jung

Uhm²,

Lim³

et al. 2011

Int J Oncol

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Abstract. Disturbance of glucose metabolism is a common feature in liver cirrhosis which is associated with insulin resistance and is an established risk factor for disease progression and survival in patients with chronic liver diseases. We investigated whether umbilical cord blood-derived mesenchymal stem cells (HMSCs) have an effect on the expression of molecules responsible for glucose utilization and hepatic gluconeogenesis, focusing on the insulin signaling pathway in rats with liver cirrhosis. Rats received a dose of CCl 4 (100 µl/100 g 4:1 in corn oil) thrice-weekly. HMSCs were infused at 4 weeks after induction of liver cirrhosis by CCl 4 . Infusion of HMSCs improved insulin resistance which was associated with increased glucose levels and decreased insulin sensitivity in CCl 4 -induced cirrhotic rats. HMSCs increased activities in the proximal part of the insulin signaling cascade, as evidenced by increased expression of key enzymes such as phosphatidylinositol-3-kinase (PI 3-kinase), protein kinase B (PKB), protein kinase C-ζ (PKC-ζ), and the decrease of glycogen synthase kinase 3 (GSK-3) compared to CCl 4 -induced liver cirrhotic rats. We also observed that glucose-6-phosphatase (G-6-P) and phosphoenolpyruvate kinase (PEPCK), two hepatic enzymes involved in gluconeogenesis were strongly decreased over 40-50% after infusion of HMSCs. Taken together, our results showed that HMSCs could improve insulin resistance in CCl 4 -induced liver cirrhosis, thereby contributing to glucose homeostasis.

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Rapid neural differentiation of human cord blood-derived mesenchymal stem cells

Cited by 83 publications

References 11 publications

Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

Differential Gene Expression Profiling of Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells by DNA Microarray

Stem cell grafts as therapeutic tools for central nervous system disorders.

Human umbilical cord blood-derived mesenchymal stem cells improve glucose homeostasis in rats with liver cirrhosis

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