Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera

Ranjbar, Reza; Naghoni, Ali; Afshar, Davoud; Nikkhahi, Farhad; Mohammadi, Mohsen

doi:10.1016/j.phrp.2016.10.002

Cited by 16 publications

(11 citation statements)

References 29 publications

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“…enteritidis ) is among the most important disease-causing serotypes. So far, more than 2,500 Salmonella serovars have been identified based on flagellar and somatic antigens, most of which are pathogenic to humans and animals ( 2 , 3 ).…”

Section: Introductionmentioning

confidence: 99%

The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle

Ranjbar¹,

Dehkordi²,

Heiat³

2020

ijph

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Background: Salmonella enteritidis causes infections in humans and animals. Antibiotics are used to eliminate bacterial infections, which become resistant to antibiotics after a while. This study aimed to isolate S. enteritidis from cattle feces samples and also to evaluate the frequency of genes associated with multi-drug resistance (MDR). Methods: One hundred ten fecal samples of cattle were collected from Jul to Dec, 2017 in Khuzestan Province, southern Iran. Bacterial culture and molecular methods were used to isolate and identify S. enteritidis. Disk diffusion method was used to assess antibiotic susceptibility. Then Polymerase chain reaction (PCR), assay was used for definitive diagnosis of S. enteritidis and resistance genes. Results: Overall, 101 (91.81%) samples were detected to be contaminated with Salmonella genus and 86 samples (85.14%) were identified as S. enteritidis. The highest and lowest antibiotic resistance were belonged to gentamicin (n=70, 81.39%), and tetracycline (n=6, 6.97%). Besides, 64 samples (74.42%) had 2-10 drugs resistance patterns. Moreover, the highest and the least resistance were related to blaIMP-1 (n=73, 84.88%) and tet(B) (n=49, 56.97%) genes respectively. Conclusion: The drug-induced genes in S. enteritidis have a high frequency. Therefore, antibiotic resistance and high MDR to antibiotics can be due to the incorrect use of antibiotics and the lack of health monitoring in Cattle farms.

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Section: Introductionmentioning

confidence: 99%

The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle

Ranjbar¹,

Dehkordi²,

Heiat³

2020

ijph

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“…and E. coli, and BHI+2% NaCl for V. parahaemolyticus. The cultures were incubated at 35±2 °C for 18-24 h (Ranjbar, Naghoni, Afshar, Nikkhahi, & Mohammadi, 2016). The bacterial cultures were diluted to obtain ± 10 1 CFU/ml and were confirmed by plating onto Tryptone Soya Agar (TSA) (Oxoid, UK) for Salmonella spp., E. coli and S. aureus; and TSA+2% NaCl for V. parahaemolyticus.…”

Section: Bacterial Culture Preparationmentioning

confidence: 99%

Rapid and Simultaneous Detection of Vibrio parahaemolyticus, Salmonella spp. and Escherichia coli in Fish by Multiplex PCR

Triwibowo

Rachmawati

Dwiyitno

2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Pathogenic bacteria are commonly found as natural contaminants in seafood and fish products. Globally, several countries have been imposing strict regulations on the maximum levels of pathogens and consequently require microbial testing of pathogens before the products can be marketed. A culture-based method with biochemical assay has been widely used to detect pathogenic bacteria in food, despite its long and extensive process. Meanwhile, the alternative molecular-based method to overcome this problem, cannot differentiate between viable and nonviable cells, which may lead to underestimation. This study aimed to develop a multiplex PCR (mPCR) method as a confirmatory assay for the culture-based method to detect pathogens in fish products simultaneously. This method applied a pre-enrichment step to ensure the growth of low-level pathogens and the injured cells in the sample. The target genes were ToxR, InvA, and UidA for Vibrio parahaemolyticus, Salmonella spp. and Escherichia coli, respectively. This assay also amplified the 16S rDNA gene of bacteria as an internal control for the PCR reaction. By implementing liquid-based DNA extraction during analysis, the developed-mPCR was comparable to detect the targeted bacteria in artificially-contaminated samples. The method was more sensitive in naturally-contaminated samples, where the number of E. coli, Salmonella spp. and V. parahaemolyticus detected were 28, 7, and 22, respectively. While the conventional method only detected 26, 5, and 19 of the respective pathogens. With a relatively shorter time and lower operation cost, the mPCR method is potential as an alternative for the culture-based method.

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“…Causes of gastrointestinal illnesses are complex, comprising virus (eg, rotavirus, norovirus and calicivirus), bacteria (eg, Escherichia coli , salmonella and shigella), parasites (eg, whipworm, amoebic dysentery and giardiasis) and chemicals (eg, alcohol, salicylic acid and medicine), as well as psychological and behavioural factors (eg, stress, handwashing and intake of un-clean water) 17–21. The symptoms of gastrointestinal illnesses also vary greatly ranging from diarrhoea, vomiting, purulent or bloody stool to abdominal pain, gastric discomfort, anorexia and dehydration, among other symptoms.…”

Section: Introductionmentioning

confidence: 99%

Pathways of healthcare and antibiotics use following reported gastrointestinal illness: a cross-sectional study in rural Anhui, China

et al. 2019

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ObjectiveTo document the factors, and their pathways, that influence healthcare and antibiotics use following reported gastrointestinal illnesses in Anhui province, China.Study designThis study uses cross-sectional design, descriptive statistical analysis, pathway mapping and multivariate logistic regression modelling.SettingHouseholds in 12 villages in Anhui province, China.ParticipantsA total of 3659 residents who: (1) held a registered rural residence and were actually living in the sampled villages when this study was conducted; (2) were aged 18 years and older and (3) were willing to participate and able to answer the survey questions.Outcome measuresPlanned and measured variables included the occurrence of gastrointestinal illness, professional care seeking and antibiotic use due to the illness and factors influencing these measures.ResultsOf the 3659 informants, 29.0% reported gastrointestinal illness episodes in the past year. Of these episodes, 50.2% led to professional care seeking and 65.4% of antibiotic use. Multivariate logistic modelling identifies that: (1) reported gastrointestinal illnesses were more frequent in north compared with middle (OR 0.569, 95% CI 0.472 to 0.686) and south (OR 0.588, 95% CI 0.492 to 0.702) Anhui, and were positively associated with age (B=0.123, p<0.05), knowledge concerning (B=0.248, p<0.05) and practice of (B=0.184, p<0.05) prevention; (2) seeking professional care and antibiotic use following the illness was linked to the severity of symptoms and geographical locations, rather than to specific pathogenic features.ConclusionsReported gastrointestinal illnesses are quite prevalent in the sample population and a large proportion of these have resulted in professional care and antibiotics use. The factors associated with the reported illnesses and pathways of healthcare and antibiotic use, as identified in this study, should inform future research and intervention efforts.

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Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera

Cited by 16 publications

References 29 publications

The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle

The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle

Rapid and Simultaneous Detection of Vibrio parahaemolyticus, Salmonella spp. and Escherichia coli in Fish by Multiplex PCR

Pathways of healthcare and antibiotics use following reported gastrointestinal illness: a cross-sectional study in rural Anhui, China

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