RAPID METHODS FOR IDENTIFYING SEAFOOD MICROBIAL PATHOGENS AND TOXINS<sup>1</sup>

Kalamaki, Mary S.; Price, Robert A.; Fung, Daniel Y. C.

doi:10.1111/j.1745-4581.1997.tb00155.x

Cited by 13 publications

(4 citation statements)

References 154 publications

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“…The organism colonizes the intestinal tract, where toxins are produced and then become active in the colon. For this reason, much research is being conducted to achieve competitive exclusion by nonpathogenic organisms Kalamaki, Price, and Fung (1997) summarized screening and identifi cation test kits for Escherichia coli in Table 1 of their article. Similar tables for the detection of Enterobacteriaceae, Campylobacter, Salmonella, Listeria, Rotavirus, Staphylococcus aureus, Vibrio cholerae, and V. vulnifi cus are presented in the same publication.…”

Section: Escherichia Colimentioning

confidence: 99%

Microbial Hazards in Foods: Food‐Borne Infections and Intoxications

Fung

2010

Handbook of Meat Processing

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Section: Escherichia Colimentioning

confidence: 99%

Microbial Hazards in Foods: Food‐Borne Infections and Intoxications

Fung

2010

Handbook of Meat Processing

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“…To include a nonenriched group, the remaining 1 ml of suspension was placed in a test tube containing 5 ml of PBS, which acts as a buffer solution against osmotic stress but provides no additional benefit to cells within the suspension. Both enriched and nonenriched samples (Kalamaki et al 1997) were incubated at 37°C for 6 h. After incubation, samples were centrifuged at 2500 g for 20 min. The pellet was extracted using a MoBio Laboratories PowerSoilÔ DNA Extraction kit (MoBio Laboratories, Inc., Carlsbad, CA, USA).…”

Section: Water and Sediment Extractionmentioning

confidence: 99%

Salmonella rarely detected in Mississippi coastal waters and sediment

Carr

Wang

McLean

et al. 2010

Journal of Applied Microbiology

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Aims: Standards for the rapid detection of individual pathogens from environmental samples have not been developed, but in their absence, the use of molecular‐based detection methods coupled with traditional microbiology techniques allows for rapid and accurate pathogen detection from environmental waters and sediment. The aim of this research was to combine the use of enrichment with PCR for detection of Salmonella in Mississippi coastal waters and sediment and observe if that presence correlated with levels of enterococci and climatological variables. Methods and Results: Salmonella were primarily found in samples that underwent nutrient enrichment and were present more frequently in freshwater than marine waters. Salmonella were detected infrequently in marine and freshwater sediments. There was a significant positive correlation between the presence of detectable Salmonella and the average enterococcal count. An inverse relationship, however, was observed between the frequency of detection and the levels of salinity, turbidity and sunlight exposure. Conclusions: Results from this study indicated the presence of Salmonella in Mississippi coastal waters, and sediments are very low with significant differences between freshwater and marine environments. Significance and Impact of the Study: Using pathogenic and novel nonpathogenic molecular markers, Salmonella do not appear to be a significant pathogenic genus along the Mississippi Coast.

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“…Consequently, the number of SSO and the concentration of their metabolites can be used as objective quality indices for shelf-life determination in seafoods [42]. are some examples of molecular techniques [42,[51][52][53][54][55][56][57][58]. (important for marine, temperate, fresh fish stored aerobically in chilled conditions).…”

Section: Microbiological/biological Methodsmentioning

confidence: 99%