Rapid method for the quantification of amoxicillin and its major metabolites in pig tissues by liquid chromatography-tandem mass spectrometry with emphasis on stability issues

Reyns, Tim; Cherlet, Marc; Baere, Siegrid De; Backer, Patrick De; Croubels, Siska

doi:10.1016/j.jchromb.2007.11.007

Cited by 69 publications

(54 citation statements)

References 35 publications

(70 reference statements)

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“…AMPC is not substantially metabolized, as 60 to 75% is excreted unchanged in urine, but some AMPC is metabolized to amoxicilloic acid and amoxicillin diketopiperazine-2Ј,5Ј-dione (40). In the last decade, numerous studies concerning either the uptake or the elimination of ␤-lactam antibiotics have been undertaken using as models in vitro-isolated hepatocytes, Xenopus oocytes, and renal cell lines expressing carrier-mediated transporters (10,23,50).…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemistry for Amoxicillin and Its Use for Studying Uptake of the Drug in the Intestine, Liver, and Kidney of Rats

Fujiwara

Shin

Miyazaki

et al. 2011

Antimicrob Agents Chemother

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Specific transport systems for penicillins have been recognized, but their in vivo role in the context of other transporters remains unclear. We produced a serum against amoxicillin (anti-AMPC) conjugated to albumin with glutaraldehyde. The antiserum was specific for AMPC and ampicillin (ABPC) but crossreacted weakly with cephalexin. This enabled us to develop an immunocytochemical (ICC) method for detecting the uptake of AMPC in the rat intestine, liver, and kidney. Three hours after a single oral administration of AMPC, the ICC method revealed that AMPC distributed to a high degree in the microvilli, nuclei, and cytoplasm of the absorptive epithelial cells of the intestine. AMPC distributed in the cytoplasm and nuclei of the hepatocytes in a characteristic granular morphology on the bile capillaries, and in addition, AMPC adsorption was observed on the luminal surface of the capillaries, intercalated portions, and interlobular bile ducts on the bile flow. Almost no AMPC could be detected 6 h postadministration in either the intestine or the liver. Meanwhile, in the kidney, AMPC persisted until 12 h postadministration to a high degree in the proximal tubules, especially in the S3 segment cells in the tubular lumen, in which numerous small bodies that strongly reacted with the antibody were observed. All these sites of AMPC accumulation correspond well to specific sites where certain transporter systems for penicillins occur, suggesting that AMPC is actually and actively absorbed, eliminated, or excreted at these sites, possibly through such certain penicillin transporters.Amoxicillin (AMPC) is a moderate-spectrum, bacteriolytic, ␤-lactam antibiotic used to treat bacterial infections caused by susceptible microorganisms, acting by inhibiting the synthesis of the bacterial cell wall. In chemotherapy, the ability of a drug to reach its site of action for a desired duration is dependent on absorption, distribution, metabolism, and excretion, all of which are intimately related to the transport mechanisms in the barrier epithelia (56). Actually, pharmacotherapeutic efficacy and toxicity are governed in vivo by a multitude of pharmacodynamic and pharmacokinetic factors. Recently, a variety of transporters for penicillins have been demonstrated at the molecular level, especially in the kidney and liver, in which numerous potentially toxic xenobiotics and drugs are eliminated (23). It has been postulated that the following may be involved in the transport of penicillins: in the small intestine, the proton-coupled oligopeptide transporter PEPT1 (1); in the liver, the organic anion transporter (OAT) (5, 22, 44, 54), multidrug resistance-associated protein (Mrp2) (6,26,39,42), and sodium-dependent phosphate transport protein (NPT1) (8, 21, 57); and in the kidney, the rat multispecific organic anion transporter 1 (rOAT1), rOAT2, and rOAT3 (2, 22, 23, 51, 55), Mrp2 (43), and H ϩ /peptide cotransporters (PEPT1 and PEPT2) (23-25, 38, 45, 49, 53), etc. The interaction of such transporters with ␤-lactam antibiotics has been stu...

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Section: Discussionmentioning

confidence: 99%

Immunocytochemistry for Amoxicillin and Its Use for Studying Uptake of the Drug in the Intestine, Liver, and Kidney of Rats

Fujiwara

Shin

Miyazaki

et al. 2011

Antimicrob Agents Chemother

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“…Li et al found that penicilloic acid is stable in the form of salts or esters in neutral solutions, however, under high temperature, acidity and in presence of b-lactamase, penicilloic acid is unstable, thereby it could not be used as a standard for accurate analysis of penicilloic acid by b-lactamase using UPLC-ESI-TOF-MS . Nevertheless, Reyns et al and De Baere et al researched that amoxicilloic acid was quite stable and could be quantitatively analysed in animal tissues (Reyns T et al, 2008;De Baere S et al, 2002). In this work, the use of penicilloic acid is in the form of salts, and the method validation showed that penicilloic acid was stable under the process of MSPD-HPLC.…”

Section: Analytesmentioning

confidence: 72%

Molecularly imprinted polymer cartridges coupled to liquid chromatography for simple and selective analysis of penicilloic acid and penilloic acid in milk by matrix solid-phase dispersion

Luo

Zheng

et al. 2015

Food and Chemical Toxicology

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“…The recoveries of AMO, AMA, DIKETO and AMP using 20 mL of acetonitrile exceeded 84.27%, 81.22%, 89.45% and 91.37%, respectively, which confirmed the reliability of this method. Reyns et al (2008) reported a method to remove proteins by a centrifugation step using a 30-kDa cut-off filter for 30 min. However, this step extended the analysis time of the egg samples and reduced the number of samples that could be analyzed within a set timeframe.…”

Section: Quality Parametersmentioning

confidence: 99%

Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry

et al. 2016

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Rapid method for the quantification of amoxicillin and its major metabolites in pig tissues by liquid chromatography-tandem mass spectrometry with emphasis on stability issues

Cited by 69 publications

References 35 publications

Immunocytochemistry for Amoxicillin and Its Use for Studying Uptake of the Drug in the Intestine, Liver, and Kidney of Rats

Immunocytochemistry for Amoxicillin and Its Use for Studying Uptake of the Drug in the Intestine, Liver, and Kidney of Rats

Molecularly imprinted polymer cartridges coupled to liquid chromatography for simple and selective analysis of penicilloic acid and penilloic acid in milk by matrix solid-phase dispersion

Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry

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