Rapid Live Bacteria Counter Using Cassette-Type Flow Cytometry

Takenaka, Kazuhiro; Sasaki, Y.; Inami, Hisao; Nakamoto, Hideki; Watanabe, Yusuke; Kurihara, Masahito; Takei, K.; Ishikawa, Jun; Miyake, Ryo

doi:10.1299/kikaic.77.401

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Many studies have been reported regarding the rapid detection of biological particles such as fungi, bacteria, and viruses in water using Micro TAS [12,13]. We even previously reported on a flow cytometry counter for viable bacteria in food suspension using a chip that included filtration and fluorescent labeling of bacteria functions [14]. However, very few Micro TAS devices have been previously reported for biological aerosol particle detection that have collecting and labeling functions.…”

Section: Introductionmentioning

confidence: 99%

Airborne virus detection by a sensing system using a disposable integrated impaction device

et al. 2016

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There are many respiratory infections such as influenza that cause epidemics. These respiratory infection epidemics can be effectively prevented by determining the presence or absence of infections in patients using frequent tests. We think that self-diagnosis may be possible using a system that can collect and detect biological aerosol particles in the patient's breath because breath sampling is easy work requiring no examiner. In this paper, we report a sensing system for biological aerosol particles (SSBAP) with a disposable device. Using the system and the device, someone with no medical knowledge or skills can safely, easily, and rapidly detect infectious biological aerosol particles. The disposable device, which is the core of the SSBAP, can be an impactor for biological aerosol particles, a flow-cell for reagents, and an optical window for the fluorescent detection of collected particles. Furthermore, to detect the fluorescence of very small collected particles, this disposable device is covered with a light-blocking film that lets only fluorescence of particles pass through a fluorescence detector of the SSBAP. The SSBAP using the device can automatically detect biological aerosol particles by the following process: collecting biological aerosol particles from a patient's breath in a sampling bag by the impaction method, labeling the collected biological aerosol particles with fluorescent dyes by the antigen-antibody reaction, removing free fluorescent dyes, and detecting the fluorescence of the biological aerosol particles. The collection efficiency of the device for microspheres aerosolized in the sampling bag was more than 97%, and the SSBAP with the device could detect more than 8.3 × 10(3) particles l(-1) of aerosolized influenza virus particles within 10 min.

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Section: Introductionmentioning

confidence: 99%

Airborne virus detection by a sensing system using a disposable integrated impaction device

et al. 2016

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“…Micro total analysis systems (micro-TAS) will be an alternative to conventional clinical auto analyzer because they have precise structure for accurate operation and small enough. As a base material for micro-TAS, Silicon, plastics and PDMS have been mainly used because of the accuracy of form, but still have cost problem for disposable usage [1]. On the other hand, paper material has been used in clinical simple test, for instance, dry chemistry and immuno-chromatography, because paper is extremely low cost material appropriate for disposable usage and suitable for burning sterilization with a little production of toxic substances.…”

Section: Introductionmentioning

confidence: 99%

Micro-liquid handling on paper analysis chip triggered by ink-jet droplet

Okabe

Sakamoto

Murakami

et al. 2011

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference

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New concept of paper-based devices (we call "paper analysis chip") and ink-jet printer-like analyzer for clinical diagnostics was presented. An original paper material of the chips (we call it "functional paper") was made by a newly developed paper-making process with adding SAP (super absorbent polymer). The quite a new liquid handling mechanism with an active injection port by accumulation of the functional paper was proposed. By using demonstrate paper chip, the validity of the mechanism was confirmed. The proto-type of paper analysis chip with two liquid handling mechanisms has been fabricated by hot-embossing process using Si-die on the functional paper.

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Rapid Live Bacteria Counter Using Cassette-Type Flow Cytometry

Abstract: We developed the rapid live bacteria counter (RLBC) with the disposable cassette to count live bacteria rapidly,

Cited by 2 publications

References 7 publications

Airborne virus detection by a sensing system using a disposable integrated impaction device

Airborne virus detection by a sensing system using a disposable integrated impaction device

Micro-liquid handling on paper analysis chip triggered by ink-jet droplet

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