Rapid LC–MS/MS detection of different carbapenemase types in carbapenemase-producing Enterobacterales

Small-molecule inhibitors of bacterial-producing metallo-β-lactamases: insights into their resistance mechanisms and biochemical analyses of their activities

“…79 Li et al proposed the application of liquid chromatography-coupled tandem mass spectrometry (LC-MS/ MS) for the effective identification of various carbapenemases in Enterobacterales. 80 This method employs the concept in which 3-aminophenyl boronic acid (APB) and EDTA inhibit Class A carbapenemases and MBLs, respectively. The principle was primarily based on the measurement of the ratio in the peak area of hydrolyzed and intact meropenem (with or without the presence of an inhibitor).…”

“…Consequently, compared to tubes A and C, Class A carbapenemases-producer had a relatively smaller peak area for tube B. Interestingly, this approach achieved 100% specificity in detecting Class A carbapenemases and MBL-producing bacteria, with a sensitivity of up to 97.64%. 80 Despite its speedy and reliable detection, the LC–MS/MS instrument is not widely available in every laboratory setting, limiting its application. However, the MDR pathogens, especially the ESKAPE family are mostly detected in clinical isolates using some of the aforementioned techniques.…”