Rapid inactivation of the yeast Sec complex selectively blocks transport of post-translationally translocated proteins

Abstract

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Copper inhibits protein maturation in the secretory pathway by targeting the Sec61 translocon in Saccharomyces cerevisiae

“…3 , D and H ). In addition, we repeatedly obtained an extra band in case of Tm treatment (represented by an asterisk ∗), which shows migration similar to the GPI-anchored form of ER Gas1p digested with EndoH ( 51 ). As can be seen in Figure 3 B , copper treatment accumulates CPY band, which moves marginally slower than the proCPY accumulated under Tm treatment ( 38 ).…”