Copper inhibits protein maturation in the secretory pathway by targeting the Sec61 translocon in Saccharomyces cerevisiae

Saha, Nitu; Tomar, Raghuvir Singh

doi:10.1016/j.jbc.2022.102170

Cited by 4 publications

(2 citation statements)

References 76 publications

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“…5B , left panel), suggesting that the addition of Cu enhanced the translational stalling of LepB-CutF. However, it was recently shown that Cu can also inhibit protein export via the Sec61 translocon ( 38 ). To exclude the possibility of the Cu-induced inhibition of the homologous SecYEG translocon, the LepB-SecM fusion was used as a control ( Fig.…”

Section: Resultsmentioning

confidence: 98%

Metabolic Sensing of Extracytoplasmic Copper Availability via Translational Control by a Nascent Exported Protein

Öztürk

Andrei

Blaby-Haas

et al. 2023

mBio

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Section: Resultsmentioning

confidence: 98%

Metabolic Sensing of Extracytoplasmic Copper Availability via Translational Control by a Nascent Exported Protein

Öztürk

Andrei

Blaby-Haas

et al. 2023

mBio

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“…5B, left panel), suggesting that Cu addition enhanced translational stalling of LepB-CutF. However, it was recently shown that Cu can also inhibit protein export via the Sec61 translocon (39). To exclude the possibility of Cu-induced inhibition of the homologous SecYEG translocon, the LepB-SecM fusion was used as a control (Fig.…”

Section: Cu Addition Decreases Co-translational Export Of Cutfmentioning

confidence: 98%

Metabolic Sensing of Extra-cytoplasmic Copper Availability via Translational Control by a Nascent Exported Protein

Öztürk

Andrei

Blaby‐Haas

et al. 2022

Preprint

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Metabolic sensing is a crucial prerequisite for cells to adjust their physiology to rapidly changing environments. In bacteria, the response to intra- and extra-cellular ligands is primarily controlled by transcriptional regulators, which activate or repress gene expression to ensure metabolic acclimation. Translational control, such as ribosomal stalling can also contribute to cellular acclimation and has been shown to mediate responses to changing intracellular molecules. In the current study, we demonstrate that co-translational export of the protein CutF regulates translation of the down-stream cutO-encoded multi-copper oxidase CutO in response to extracellular copper (Cu). Our data show that CutF, acting as a Cu sensor, is co-translationally exported by the signal recognition particle pathway. Binding of Cu to the periplasmically exposed Cu-binding motif of CutF delays its co-translational export via its C-terminal ribosome stalling-like motif. This allows the unfolding of an mRNA stem-loop sequence that shields the ribosome-binding site of cutO, which favors its subsequent translation. Bioinformatic analyses reveal that CutF-like proteins are widely distributed in bacteria and often, are located upstream of genes involved in transition metal homeostasis. Our overall findings illustrate a highly conserved control mechanism using co-translational export of a protein acting as a sensor to integrate the changing availability of extracellular nutrients into metabolic acclimation.

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Dual-recognition “turn-off-on” fluorescent Biosensor triphenylamine-based continuous detection of copper ion and glyphosate applicated in environment and living system

Liu,

Zhang,

Yao

et al. 2024

Journal of Hazardous Materials

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Copper inhibits protein maturation in the secretory pathway by targeting the Sec61 translocon in Saccharomyces cerevisiae

Cited by 4 publications

References 76 publications

Metabolic Sensing of Extracytoplasmic Copper Availability via Translational Control by a Nascent Exported Protein

Metabolic Sensing of Extracytoplasmic Copper Availability via Translational Control by a Nascent Exported Protein

Metabolic Sensing of Extra-cytoplasmic Copper Availability via Translational Control by a Nascent Exported Protein

Dual-recognition “turn-off-on” fluorescent Biosensor triphenylamine-based continuous detection of copper ion and glyphosate applicated in environment and living system

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