Rapid in vitro shoot regeneration of Valeriana officinalis

Abstract

A protocol for efficient and rapid regeneration of medicinal plant (Valeriana officinalis L.) was developed by in vitro culture of crown division. The effect of three different culture media, MS, B5 and MB (a new combined medium) containing 12 combinations of BAP and NAA was evaluated on regeneration and growth parameters. Results revealed a significant response of media used for shoot regeneration. The best frequency of regenerated plantlets (80%) and number of regenerated shoots (9.43) were obtained on MB containing BAP (0.5 mg/l) + (0.25 mg/l). The maximum length of shoots was found in the MB with BAP (1 mg/l) + (0.5 mg/l). A positive influence of time was noticed on rooting of regenerated shoots. All acclimatized plantletswere successfully established in a greenhouse and showed normal growth. This protocol provides a technique for mass propagation of valerian and helpful for future genetic improvement studies.

In vitro propagation and evaluation of Valtrate and Valereinic acid in Valerina officinalis L.

“…The obtained results clearly showed that the high percentage of acclimatized and survival plantlets 73.67±5.55 a was recorded with culturing of in vitro derived plantlets in nutrient media containing peat-moss and vermiculite at 1:1 as shown Fig. (13,14). However, the minimum number of acclimatized and survival plantlets 36.33±3.48 b were recorded with culturing of in vitro derived plantlets on sand media.…”

“…The obtained regenerated shoots derived from callus of leaf explant have shown that the best-regenerated media affected on a percentage of regenerated shootlets, length of shoot, several shoots, number of the leaf, and length of plantlets were MS+3ppm BA+1ppm 2,4-D (33±4.04 a ,14.67±0.77 a , 1.67±0.33 a , 2.67±0.33 a , 20.67±1.76 a ). Whereas Tansaz et al, 2014 [14]reported that BA (0.5 mg/l) +NAA (0.25mg/lon MB(half (MS+B5) salts and vitamins, 10 ml/l Fe EDTA (iron stock) + 2.5% sucrose) achieved of shoot length using MB with BA (1 mg/l) + (0.5 mg/l). Adversely to Ghaderi & Jafari 2014 [11]who have shown that media supplemented with Thidiazuron(TZD) and NAA have marked regeneration yield from petiole and leaf explants.…”

“…However, regeneration was accomplished by using Indole butyric acid (IBA) and BA, which had favourable effects on plant regeneration and shoot induction [1]. However, utilizing BA (1mg/l) or (0.5mg/l) resulted in the longest shoots and roots were detected [13,14]. on the other hand, Zebarjadi et al, 2011 [15] recommended that IBA was an acceptable hormone for root growth in addition to employing pasteurized field soil, sand, and perlite for effective acclimatization.…”