Rapid in vitro propagation of Lucas aspera Spreng. A potential multipurpose Indian medicinal herb

Vijendra, Poornima D.; Jayanna, Sathisha G.; Kumar, Vadlapudi; Gajula, Hari; Rajashekar, J.; Sannabommaji, Torankumar; Basappa, Giridhara; Anuradha, C. M.

doi:10.1016/j.indcrop.2017.05.042

Cited by 15 publications

(4 citation statements)

References 30 publications

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“…In a study by Slupski et al [ 14 ], high BAP concentrations (20 μM) markedly reduced the survival rate of C. pilosula . Consistent with our finding, Vijendra et al [ 36 ] have reported 1.5 mg·L −1 BAP as the optimal concentration for shoot formation (6.9 shoots per explant) in Lucas aspera Spreng. However, some studies have reported lower or higher optimal concentrations of BAP for shoot proliferation.…”

Section: Discussionsupporting

confidence: 93%

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

et al. 2023

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Background Codonopsis pilosula (Franch.) Nannf. is a medicinal plant traditionally used in China, Korea, and Japan to treat many diseases including poor gastrointestinal function, low immunity, gastric ulcers, and chronic gastritis. The increasing therapeutic and preventive use of C. pilosula has subsequently led to depletion of the natural populations of this species thus necessitating propagation of this important medicinal plant. Here, we developed an efficient and effective in vitro propagation protocol for C. pilosula using apical shoot segments. We tested various plant tissue culture media for the growth of C. pilosula and evaluated the effects of plant growth regulators on the shoot proliferation and rooting of regenerated C. pilosula plants. Furthermore, the tissues (roots and shoots) of maternal and in vitro-regenerated C. pilosula plants were subjected to Fourier-transform near-infrared (FT-NIR) spectrometry, Gas chromatography-mass spectrometry (GC–MS), and their total flavonoids, phenolics, and antioxidant capacity were determined and compared. Results Full-strength Murashige and Skoog (MS) medium augmented with vitamins and benzylaminopurine (1.5 mg·L−1) regenerated the highest shoot number (12 ± 0.46) per explant. MS medium augmented with indole-3-acetic acid (1.0 mg·L−1) produced the highest root number (9 ± 0.89) and maximum root length (20.88 ± 1.48 mm) from regenerated C. pilosula shoots. The survival rate of in vitro-regenerated C. pilosula plants was 94.00% after acclimatization. The maternal and in vitro-regenerated C. pilosula plant tissues showed similar FT-NIR spectra, total phenolics, total flavonoids, phytochemical composition, and antioxidant activity. Randomly amplified polymorphic DNA (RAPD) test confirmed the genetic fidelity of regenerated C. pilosula plants. Conclusions The proposed in vitro propagation protocol may be useful for the rapid mass multiplication and production of high quality C. pilosula as well as for germplasm preservation to ensure sustainable supply amidst the ever-increasing demand.

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Section: Discussionsupporting

confidence: 93%

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

et al. 2023

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“…A high BAP to low NAA concentration ratio gave the highest plantlet formation (Table 4). A similar result on high BAP to low NAA combination has been reported by Vijendra et al (2017) for Mentha piperitau, though shoot-tip and nodal explants were used. Further, a combination of BAP and NAA in MS basal medium has been reported in inducing shoots from different sources of explants, such as embryos in C. scabiosus (Salamma and Rao 2013) and callus induction and shoot regeneration of Lantara camara, a medicinal plant (Veraplakron 2016).…”

Section: Initiation Of Cultures From Seedssupporting

confidence: 72%

Effective decontamination and multiplication of Croton membranaceus in vitro

2022

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Abstract. Adukonu IA, Ayeh KO, Acheampong E. 2022. Effective decontamination and multiplication of Croton membranaceus in vitro. Cell Biol Dev 6: 68-81. Croton membranaceus Müll. Arg. is a useful herb with medicinal properties, and its leaves, roots, and bark are used to treat diverse ailments. However, the harvesting method by traditional medical practitioners without replacement exposes it to extinction. Therefore, the only means of propagating is by relatively slow use of seeds, and an alternative propagation method is needed for field establishment and nursery. Therefore, this study aims at determining an effective propagation by sterilization regime and subsequent in vitro regeneration using different explants; intact seeds, isolated embryos, coatless seeds, and nodal-cutting. The explants were decontaminated using double sterilization; the best was achieved by pre-treatment with 70% ethanol for 3 min before immersion in sodium hypochlorite (NaOCl). Intact seeds were effectively decontaminated by immersion in 15% NaOCl solution for 20 min, then by 10% NaOCl for 15 min. Conversely, coatless seeds were effectively decontaminated when isolated from intact seeds immersed in 20% NaOCl solution for 20 min and 15% NaOCl for 15 min. Further, embryos isolated from intact seeds were effectively decontaminated in 20% NaOCl for 15 min, then by 15% NaOCl for 10 min sequentially. These sterilization regimes successfully decontaminated 86% intact seeds, 100% isolated embryos, and 80% coatless seeds. Nodal-cutting explants were best decontaminated by immersion in 20% NaOCl solution for 15 min, followed by 15% NaOCl for 10 min sequentially without ethanol pre-treatment. This sterilization regime successfully decontaminated 100% of the nodal-cutting explants. However, the development of shoot explants varied in response to sterilization. Intact seeds did not develop into shoots, while isolated embryos, coatless seeds, and nodal-cutting explants developed into shoots independent of the sterilization regime. Shoot development was highest with the medium’s shoot-tip explants added with BAP, NAA, and GA3. Shoot multiplication was best achieved on an MS basal medium with 5.0 ?M BAP, 0.5 ?M NAA, and 5.0 ?M GA3 amendment.

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“…The critical parameters for the efficiency of TIS in terms of multiplication rate and yield are the frequency and duration of immersion [21,23,32]. Many researchers have attempted to establish the optimal procedures for the mass propagation and biosynthesis of secondary metabolites in medicinal plants such as Eurycoma longifolia [33], Rhododendron tomentosum [34], Lucas aspera [35], Stevia rebaudiana [36], and Anthurium andreanum [37].…”

Section: Discussionmentioning

confidence: 99%

Optimal Growth and Biomass of Centella asiatica Using a Twin-Bottle Temporary Immersion Bioreactor

2023

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Centella asiatica or gotu kola has long been used as a traditional medicinal plant. Here, immersion times and culture systems on growth and biomass production of C. asiatica were investigated using a twin-bottle, temporary immersion system. Results indicated that all immersion times gave 100% survival, with a 5 min immersion 12 times/day, providing the highest number of new shoots (3.6 shoots/explant), leaves (10.2 leaves/explant), roots (8.3 roots/explant), and fresh and dry weights of clumps (5.06 g fresh weight and 0.48 g dry weight/clump). The temporary immersion system resulted in more than a three-fold increase in biomass accumulation, with the highest average number of new shoots, leaves, and roots compared to a semi-solid system.

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Rapid in vitro propagation of Lucas aspera Spreng. A potential multipurpose Indian medicinal herb

Cited by 15 publications

References 30 publications

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

Effective decontamination and multiplication of Croton membranaceus in vitro

Optimal Growth and Biomass of Centella asiatica Using a Twin-Bottle Temporary Immersion Bioreactor

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