Rapid in Vitro Callogenesis and Phytochemical Screening of Leaf, Stem and Leaf Callus of Mussaenda Frondosa Linn. - A Medicinal Plant.

Abstract

Objective: To standardize the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem, and callus cultures. Methods:The leaf explants were inoculated onto Murashige and Skoog medium supplemented with varying concentrations of growth regulators such as 2, 4-D, NAA, benzylaminopurine, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoid and alkaloid contents of leaf, stem, and callus were tested by standard methods. The antioxidant activities were investigated using 1, 1-diphenyl-2-picryl hydrazyl radical scavenging method and reducing power assay. The anti-inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable, and fast growing callus were obtained on the medium enriched with NAA (2 mg/l)+Kn (4 mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10±1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10 g of extract). The methanolic leaf extract exhibited the highest free radical scavenging activity with an inhibitory concentration 50% (IC 50 ) value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%). Conclusion:This preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.

Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro grown callus of Mussaenda frondosa L.: characterization and their applications

“…The surface sterilization and establishment of callus cultures were carried out from the leaf explants of M. frondosa (Manasa et al 2017). The healthy, friable callus was obtained from the MS medium supplemented with 1-naphthalene acetic acid (NAA) + kinetin (Kn) (2 + 4 mg/l).…”

“…The antioxidant activity is due to the electrostatic attraction between bioactive compounds (COO − ) and ZnO (ZnO = Zn 2+ + O 2− ) of M. frondosa (Kumar et al 2010). Phenolic compounds and flavonoids associated with M. frondosa have the ability to quench free radical owing to its antioxidant activity (Manasa et al 2017). Kumar et al (2015) is also of the opinion that the nanoparticles that…”

“…Crude extract of the plant contains important bioactive principles: phenols, flavonoids, alkaloids, steroids, glycosides, tannins, etc. (Manasa et al 2017). From time immemorial, the herbal preparations of this plant were extensively used in the treatment of asthma, fever, cough, leprosy, wounds, jaundice and so on (Kirtikar and Basu 1987).…”