Rapid Identification of Unstable Acyl Glucoside Flavonoids of Oxytropis racemosa Turcz by High‐performance Liquid Chromatography–Diode Array Detection–Electrospray Ionisation/Multi‐stage Mass Spectrometry

Abstract

The method described is effective for obtaining a comprehensive phytochemical profile of plants containing unstable acylated flavonoids. The method is also useful for constructing the chromatographic fingerprint of the minority medicine -O. racemosa Turcz for quality control.

Comprehensive Phytochemical Characterization of Herbal Parts from Kidney Vetch (Anthyllis vulnerariaL.) by LC/MSnand GC/MS

“…Neutral losses of 42, 176 and 144 Da in their MS n spectra indicated these compounds to bear acetyl (acetic acid), feruloyl (ferulic acid, 62 ) or 3‐hydroxy‐3‐methylglutaroyl (meglutol, 52 ) units, respectively ( Table 1, Figure 2 B , C ). Interestingly, meglutol‐containing flavonoids are rare in the plant kingdom, [30,32,33] and only a few have been detected in the Fabaceae family so far, e. g ., in Astragalus monspessulanus and Oxytropis species [30,31,34,35] …”

Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent‐separation strategy based on liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

“…P20 (1131.5202, C 54 H 83 O 25 ), P21 (1293.5756, C 60 H 93 O 30 ), P31 (1115.5264, C 54 H 83 O 24 ), and P36 (1319.5915, C 62 H 95 O 30 ) yielded product ions at m/z 987.4550, 1149.5328 ( P16 , segetoside C), 971.4865, and 1175.5497 ( P28 , segetoside B), respectively. These components all lost a 3‐hydroxy‐3‐methylglutaric acid (HMG, 144 Da) moiety, which is a common fragment of saponins in Wang‐Bu‐Liu‐Xing . Thus, P20 , P21 , P31 , and P36 were considered to be the HMG group homologs of monodesmosidic saponins.…”

“…These components all lost a 3-hydroxy-3methylglutaric acid (HMG, 144 Da) moiety, which is a common fragment of saponins in Wang-Bu-Liu-Xing. [33,34] Thus, P20, P21, P31, and P36 were considered to be the HMG group homologs of monodesmosidic saponins. P37 was a methyl aldopentose derivative of P31 with an elemental composition of C 60 H 93 O 28 (Table 1 and Fig.…”

Chemiluminescence of off‐line and on‐line gold nanoparticle‐catalyzed luminol system in the presence of flavonoid

“…However, because of low bioavailability, the concentrations of flavonoids and their metabolites in biological samples can be very low, which poses stringent requirements on the sensitivity of the analytical methods. At present, reported methods for the determination of flavonoids include HPLC, spectrofluorimetry, thin‐layer chromatography, liquid chromatography mass spectrometry and gas chromatography mass spectrometry . In addition, flavonoids can be determined based on the inhibition or enhancement of the luminescent signal of a luminol CL system .…”