Identification of multiple constituents from seed of <i>Vaccaria segetalis</i> with an adsorbent‐separation strategy based on liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Pan, Qi; Zhang, Feng; Xue, Rui; Li, Zhixiong; Chen, Mingcang; Sun, Zhaolin; Zhu, Kaicheng; Huang, Chenggang

doi:10.1002/rcm.6893

Cited by 12 publications

(12 citation statements)

References 37 publications

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“…Therefore, comprehensive identification and quality control of the chemical components in TCMs are important for revealing the therapeutic mechanism and ensuring their safety. However, TCMs are often difficult to identify clearly because hundreds of various chemical constituents are present in these complex systems, and some are at lower concentration levels . So it is very important to develop sensitive and reliable analytical methods for the identification and detection of TCMs …”

Section: Introductionmentioning

confidence: 99%

“…However, TCMs are often difficult to identify clearly because hundreds of various chemical constituents are present in these complex systems, and some are at lower concentration levels. [5] So it is very important to develop sensitive and reliable analytical methods for the identification and detection of TCMs. [6] In recent years, LC-MS-based techniques with excellent sensitivity and specificity have been proven to be powerful approach to simultaneously characterize and determine the different constituents in TCM researches.…”

Section: Introductionmentioning

confidence: 99%

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Identification and determination of the major constituents in Kai‐Xin‐San by UPLC‐Q/TOF MS and UFLC‐MS/MS method

Sui

et al. 2016

J. Mass Spectrom.

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In order to have overall chemical material information of Kai-Xin-San (KXS), the reliable ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometer (UHPLC-Q-TOF-MS) and ultra-fast liquid chromatography mass spectrometer (UFLC-MS/MS) methods were developed for the identification and determination of the major constituents in KXS. Moreover, the UHPLC-Q-TOF-MS method was also applied to screen for multiple absorbed components in rat plasma after oral administration of KXS. The UHPLC-Q-TOF-MS method was achieved on Agilent 6520 Q-TOF mass and operated in the negative ion mode. Good separation was performed on a ZORBAX Eclipse Plus C18 column with a gradient elution at a flow rate of 0.2 ml/min. A total of 92 compounds in KXS were identified or tentatively characterized based on their exact molecular weights, fragmentation patterns, and literature data. A total of 26 compounds including 23 prototype components and three metabolites were identified in rat plasma after oral administration of KXS. Then, 16 major bioactive constituents were chosen as the benchmark substances to evaluate the quality of KXS. Their quantitative analyses were performed by a triple quadrupole tandem mass spectrometer (MS/MS) operating in multiple-reaction monitoring mode(MRM). The analysis was completed with a gradient elution at a flow rate of 0.4 ml/min within 35 min. The simple and fast method was validated and showed good linearity, precision, and recovery. Furthermore, the method was successful applied for the determination of 16 compounds in KXS. All results would provide essential data for identification and quality control of active chemical constituents in KXS. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and determination of the major constituents in Kai‐Xin‐San by UPLC‐Q/TOF MS and UFLC‐MS/MS method

Sui

et al. 2016

J. Mass Spectrom.

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“…The seed of this plant is used for the promotion of diuresis and milk secretion, activation of blood circulation and the relief of carbuncle. Some compounds were separated from Vaccaria segetalis (Qi et al 2014;Wang et al 2011), but to date the active compound has still not been known, and there are few reports on the lactogenic mechanism of Vaccaria segetalis. In our previous reports, we found that Vaccaria segetalis promotes lactogenesis by enhancing JAK-STAT5 and mTOR pathways in dairy cow mammary epithelial cells (DCMECs) Tong et al 2011), but how Vaccaria segetalis excerts its role is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Vaccaria segetalisactivates cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells in vitro

Nan¹,

Wei²,

Hai-yang³

et al. 2015

Ind. Jour. of Anim. Rese.

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Vaccaria segetalis has very strong effect on lactogenesis, but the mechanism is still unknown. This study aims to reveal the influence of Vaccaria segetalis on cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells (DCMECs). In this study the purified DCMECs was used to screen the most effective concentration of Vaccaria segetalis water decoction for lactogenesis. DCMECs were treated with prolactin (PRL), E (estrogen) or water extraction of Vaccaria segetalis, and many lactogenesis signal molecules in DCMECs including prolactin receptor (PRLR), estrogen receptor  (ER), p-STAT5, p-mTOR, p100, GARS, PPAR and cyclinD1 were detected by using western blot assay. It was found that Vaccaria segetalis, E and PRL could promote lactogenesis of DCMECs and up-regulate these lactogenesis signal molecules except PPAR. These data reveals that Vaccaria segetalis has a similar role as E or PRL and activates cellular lactogenesis signaling pathway of DCMECs on both the translational and transcriptional levels.

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“…1) is a novel cyclic hexapeptide [cyclo-(Gly-Val-Pro-Val-Trp-Ala)] and a chief cyclic peptide component of V. segetalis seeds . According to the literature, very few analytical methods have been reported for the analysis of segetalin A in V. segetalis (Wang et al, 2011;Dong et al, 2012;Qi et al, 2014). The first method involved a high-speed countercurrent chromatography to separate cyclic peptides from the seeds of V. segetalis (Wang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…A conventional HPLC-PDA method was employed to assay segetalin A in the plant using a mobile phase of acetonitrile-water with gradient elution (Dong et al, 2012). The third method involved liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/QTOF-MS/MS) to identify the multiple constituents of V. segetalis (Qi et al, 2014). Recently, LC/QTOF-MS/MS has been investigated for the in vivo metabolism and tissue distribution of V. segetalis containing segetalin A (Qi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A sensitive and selective LC‐MS/MS method for the quantitative determination of segetalin A from the plasma of rats

Wang

Zhang

Pang

et al. 2015

Biomedical Chromatography

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A sensitive and selective LC-MS/MS method was developed and validated for the determination and pharmacokinetic investigation of segetalin A in rat plasma. Sample preparation was accomplished through a simple SPE procedure for the removal and preconcentration of the analyte and IS. Plasma samples were separated by HPLC on a Symmetry C18 column using a mobile phase consisting of methanol and 0.1% formic acid in water (70:30, v/v) with isocratic elution. The quantification was performed using multiple reaction monitoring with the transitions m/z 610.3 → 511.2 for segetalin A and m/z 779.4 → 751.4 for IS, respectively. The calibration curve was linear over the range of 8.0-4000 ng/mL with a limit of quantitation (LOQ) of 8.0 ng/mL. This method was applied in a pharmacokinetic study of segetalin A in rats. For intravenous (i.v.) administration, the plasma concentrations of segetalin A decreased quickly (t1/2z, 1.31 ± 0.341 h). For oral administration, the plasma concentrations of segetalin A increased to a peak value at 1.50 ± 0.577 h, followed by a gradual decrease to the LOQ in 12 h. The mean AUC values after i.v. and oral administration were 553 ± 105 and 1482 ± 110 ng h/mL, respectively.

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Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent‐separation strategy based on liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Cited by 12 publications

References 37 publications

Identification and determination of the major constituents in Kai‐Xin‐San by UPLC‐Q/TOF MS and UFLC‐MS/MS method

Identification and determination of the major constituents in Kai‐Xin‐San by UPLC‐Q/TOF MS and UFLC‐MS/MS method

Vaccaria segetalisactivates cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells in vitro

A sensitive and selective LC‐MS/MS method for the quantitative determination of segetalin A from the plasma of rats

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