Rapid identification of Staphylococcus epidermidis.

Wieser, Monika; Busse, Hans‐Jürgen

doi:10.1099/00207713-50-3-1087

Cited by 92 publications

(78 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From a collection of 35 isolates with highly similar protein patterns, including strain V2-BIII-A2 T , which were isolated in October 1999 from the wall painting exposed in the chapel Virgilkapelle, strains V2-BI-09, V2-BI-A4 and V2-BII-A8 were selected for further taxonomic characterization. Comparison of genomic fingerprints of these four strains obtained after ERIC (enterobacterial repetitive intergenic consensus)-PCR (Wieser & Busse, 2000) supported their affiliation to a single species and clearly distinguished them from selected reference strains (Fig. 1).…”

mentioning

confidence: 99%

Bacillus barbaricus sp. nov., isolated from an experimental wall painting

Täubel

Kämpfer

Buczolits

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

mentioning

confidence: 99%

Bacillus barbaricus sp. nov., isolated from an experimental wall painting

Täubel

Kämpfer

Buczolits

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

“…In order to detect genotypic differences between strains GH2-4 T and GH2-5, they were subjected to repetitivesequence-based PCRs, including ERIC-, BOX-and REP-PCR, as described previously (Wieser & Busse, 2000;Louws et al, 1994). The genomic fingerprints of strain GH2-4 T generated after ERIC-, BOX-and REP-PCR were identical to those of strain GH2-5.…”

mentioning

confidence: 99%

Bacillus solimangrovi sp. nov., isolated from mangrove soil

Lee

Rhee

Chang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two novel bacterial strains, GH2-4 T and GH2-5, were isolated from mangrove soil near the seashore of Weno island in Chuuk state, Micronesia, and were characterized by a polyphasic approach. The two strains were strictly aerobic, Gram-staining-positive, motile, endospore-forming rods that were catalase-and oxidase-positive. Colonies were circular, convex, stringy and transparent yellowish (GH2-4 T ) or opaque whitish (GH2-5). The 16S rRNA gene sequences of the two isolates were identical. The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus kochii WCC 4582 T , B. horneckiae DSM 23495 T , B. azotoformans LMG 9581 T , B. cohnii DSM 6307 T and B. halmapalus DSM 8723 T (95.6, 95.4, 95.4, 95.2 and 95.2 % similarity, respectively). The partial groEL sequence of strain GH2-4 T was identical to that of strain GH2-5 and showed ,85 % similarity to those of the most closely related strains. The isolates grew at pH 5-12 (optimal growth at pH 9), at 10-40 6C (optimum 30-35 6C) and at 0-9 % (w/v) NaCl (optimum 1-3 % NaCl). The cell-wall peptidoglycan of strains GH2-4 T and GH2-5 contained meso-diaminopimelic acid and cell-wall hydrolysates contained ribose as a major sugar. The DNA G+C content was 36 mol%, and DNA-DNA relatedness between the isolates and five related reference strains was 20-24 %. Strain GH2-4 T exhibited 81 % DNA-DNA relatedness with strain GH2-5. The major cellular fatty acids of both strains were iso-C 15 : 0 , iso-C 16 : 0 , iso-C 14 : 0 and anteiso-C 15 : 0 and the predominant menaquinone was MK-7. On the basis of the evidence from this polyphasic study, strains GH2-4 T and GH2-5 (5KCTC 331435JCM 189955DSM 27084) represent a novel species of the genus Bacillus, for which the name Bacillus solimangrovi sp. nov. is proposed; the type strain is GH2-4 T (5KCTC 33142 T 5JCM 18994 T 5DSM 27083 T ).Mangroves are woody plants located at the interface between land and sea. They represent a typical ecosystem in tropical and subtropical coastal biomes, and they play significant roles as feeding, breeding and refuge areas for diverse organisms and maintain a considerable food web based on their detritus (Holguin et al., 2001;Kathiresan & Bingham, 2001). The mangrove ecosystem is distinguished from others because of periodic tidal flooding and changeable environmental factors, such as salinity and nutrient availability (Alongi, 1988;Holguin et al., 2006). There have been many reports regarding the isolation of novel micro-organisms from mangrove-related environments. At the time of writing, 63 genera and 113 species had been newly described as novel micro-organisms isolated from mangrove environments, based on searches of PubMed (http://www.ncbi.nlm.nih. gov/). They include species of the yeast genus Candida The genus Bacillus, in the phylum Firmicutes, is a large group of rod-shaped, endospore-forming bacteria that includes more than 200 species (Euzéby, 2010). Since the genus Bacillus was described by Cohn in 1872, the number of species in the genus has increased considerabl...

show abstract

“…For example, several genomic targets have been effectively used for the identification of Staphylococcus species, including the 16S rRNA gene (Bialkowska-Hobrzanska et al, 1990;De Buyser et al, 1992), the tRNA gene intergenic spacer (Maes et al, 1997), the internal transcribed spacer (Couto et al, 2001), the heat-shock protein 60 (HSP60) gene (Goh et al, 1996), the chaperonin 60 gene (Goh et al, 1997), the femA gene (Vannuffel et al, 1999), the sodA gene (Poyart et al, 2001), the gap gene (Yugueros et al, 2000) and the nuc gene (Brakstad et al, 1992). Recently, an enterobacterial repetitive intergenic consensus PCR and BOX-PCR were also used in the identification of Staphylococcus epidermidis strains (Wieser & Busse, 2000). .…”

mentioning

confidence: 99%

Identification of staphylococci by 16S internal transcribed spacer rRNA gene restriction fragment length polymorphism

Sudağıdan

Yenidünya

Güneş

2005

Journal of Medical Microbiology

View full text Add to dashboard Cite

The capacity of 16S internal transcribed spacer (16S-ITS) rRNA gene RFLP to differentiate 16 type strains and nine clinical isolates of staphylococci was evaluated. The 16S rRNA gene was amplified together with the ITS region and the amplification products were digested with TaqI restriction enzyme. Analysis of the 16S-ITS rRNA gene RFLP profiles differentiated each of the 16 type strains into distinct RFLP haplotypes. INTRODUCTIONThe habitat of staphylococci is skin, skin glands and mucous membranes of humans and many animals. The genus Staphylococcus includes both pathogenic and saprophytic strains that have been isolated from animal products such as meat and milk, and from environmental sources such as soil, sea water, fresh water, dust and air samples (Kloos et al., 1991).Coagulase-positive species such as Staphylococcus aureus are the cause of many types of infection (Forbes et al., 1998). During the last two decades, coagulase-negative staphylococci (CNS) have also emerged as pathogens causing medical-device-related infections (von Eiff et al., 2002). Because of the pathogenic potential of these bacteria, effective methods are required for their in vitro identification. Beside fatty acid analyses, many diagnostic tests rely on a miniaturized phenotypic characterization and a set of biochemical reactions. These methods enable the identification of S. aureus isolates, but often fail in the identification of CNS (Stoakes et al., 1994;Renneberg et al., 1995;Martineau et al., 1996). Analysis of specific regions of genomic DNA, on the other hand, has produced much more discriminative data. For example, several genomic targets have been effectively used for the identification of Staphylococcus species, including the 16S rRNA gene (Bialkowska-Hobrzanska et al., 1990;De Buyser et al., 1992), the tRNA gene intergenic spacer (Maes et al., 1997), the internal transcribed spacer (Couto et al., 2001), the heat-shock protein 60 (HSP60) gene (Goh et al., 1996), the chaperonin 60 gene (Goh et al., 1997), the femA gene (Vannuffel et al., 1999), the sodA gene (Poyart et al., 2001), the gap gene (Yugueros et al., 2000) and the nuc gene (Brakstad et al., 1992). Recently, an enterobacterial repetitive intergenic consensus PCR and BOX-PCR were also used in the identification of Staphylococcus epidermidis strains (Wieser & Busse, 2000). . The nine clinical isolates were S. capitis subsp. capitis (n ¼ 2), S. caprae (n ¼ 2), S. cohnii subsp. cohnii (n ¼ 1) and S. epidermidis (n ¼ 4). The clinical isolates were identified by basic phenotypic tests and the Staph ID 32 system (bioMérieux). All strains were cultured in tryptic soy broth (TSB; Merck) at 37 8C and stored in 25 % (v/v) glycerol at À80 8C.16S-ITS rRNA gene PCR. Genomic DNA was isolated as described by Arciola et al. (2001). Briefly, 100 ìl of overnight culture prepared in 5 ml TSB at 37 8C with shaking was pelleted by centrifugation. Cell pellets were resuspended in 45 ìl deionized water and 5 ìl lysostaphin solution (100 ìg ml À1 in dH 2 O; Sigma) and incubated for 10 min...

show abstract

Rapid identification of Staphylococcus epidermidis.

Cited by 92 publications

References 24 publications

Bacillus barbaricus sp. nov., isolated from an experimental wall painting

Bacillus barbaricus sp. nov., isolated from an experimental wall painting

Bacillus solimangrovi sp. nov., isolated from mangrove soil

Identification of staphylococci by 16S internal transcribed spacer rRNA gene restriction fragment length polymorphism

Contact Info

Product

Resources

About