Rapid identification of neurotropic viruses by an immunofluorescent technique applied to cerebrospinal fluid cellular deposits

Sommerville, R. G.

doi:10.1007/bf01241992

Cited by 30 publications

(10 citation statements)

References 5 publications

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“…1, 2 and 3). Demonstration of viral antigens in CSF cells have been employed before and can be considered as evidence for viral meningoencephalitis (Lindeman 1974, Sommerville 1966, Dayan 1973.…”

Section: Resultsmentioning

confidence: 99%

Viral meningoencephalitis and head injury

Peters

Versteeg²,

Lindeman

et al. 1978

Acta Neurologica Scandinavica

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Among 162 children referred with head injury (in a 3-year period) a viral meningoencephalitis was proven to be present in seven cases. Only one patient showed symptoms of infection before the accident. On admission classical signs of viral meningoencephalitis were absent or mixed with the complications of head injury. A diagnosis of viral meningitis/meningoencephalitis was documented by immunofluorescent demonstration of viral antigens in CSF cells and/or pattern of CSF cytology and/or conventional serological methods. Whether the trauma and the infection are coincidental or causally related can not be decided definitively. A hypothesis is presented suggesting diminished alertness in subclinical viral meningoencephalitis, leading to accident proneness.

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Section: Resultsmentioning

confidence: 99%

Viral meningoencephalitis and head injury

Peters

Versteeg²,

Lindeman

et al. 1978

Acta Neurologica Scandinavica

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“…The mean TdR kinase specific activity of 0.64 from infected rabbit CSF at pH 6.0 is greater than the TdR kinase specific activity of 0.38 from peripheral rabbit mononuclear cells at (1,3,8,11,17) and demonstration of HVH antigen in CSF with indirect immunofluorescent procedures (7,16). It appears that a more appropriate diagnostic approach would be the detection of early nonvirion products of viral replication in CSF utilizing either sensitive enzyme assays or immunologic procedures.…”

Section: R a B B I T Smentioning

confidence: 99%

Thymidine Kinase Activity in Cerebrospinal Fluid of Rabbits with Herpesvirus hominis Encephalitis

et al. 1975

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Extractdivloid fibroblast (W1-38 cells) and the virus was identified as HVH type 1 (HVH-1) by D;. AndrB J. Nahmias of Emory The rabbit model of Herpesvirus horninis (HVH) encepha-University. litis was utilized to determine whether thymidine (TdR) kinase (EC. 2.7.1.2.1), an early enzyme in the HVH replicative cycle, CELL CULTUREis present in the cerebrospinal fluid (CSF) of rabbits with HVH encephalitis. The mean TdR kinase activity in CSF from W1-38 human diploid fibroblasts were maintained in Eagles infected rabbits was 2.30 f 1.69 pmol product/l20 min/100 pl minimum essential medium (Eagle's MEM) which contained CSF, while the mean TdR kinase activity of uninfected CSF 2% fetal calf serum, NaHC03 30 mEq/liter, penicillin 250 was 0.34 + 0.25. The enzyme appeared to be cell associated units/ml, streptomycin 200 pg/ml, polymyxin 12.5 units/ml, since the TdR kinase activity in the sedimented cell suspension and chlortetracycline 25 pg/ml.

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“…Immunofluorescent diagnosis of herpes encephalitis was first reported by Sabin & Messore (1961), using necropsy material, and by Sommerville (1966) using lymphocytes in lumbar fluid. The value of the last approach has never been confirmed and, in my experience, it is unreliable.…”

mentioning

confidence: 99%

Immunofluorescence in the diagnosis of herpes encephalitis

Longson¹

1973

Postgraduate Medical Journal

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Rapid identification of neurotropic viruses by an immunofluorescent technique applied to cerebrospinal fluid cellular deposits

Cited by 30 publications

References 5 publications

Viral meningoencephalitis and head injury

Viral meningoencephalitis and head injury

Thymidine Kinase Activity in Cerebrospinal Fluid of Rabbits with Herpesvirus hominis Encephalitis

Immunofluorescence in the diagnosis of herpes encephalitis

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