Immunofluorescence in the diagnosis of herpes encephalitis

Longson, Maurice

doi:10.1136/pgmj.49.572.403

Cited by 7 publications

(2 citation statements)

References 5 publications

(3 reference statements)

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“…77030. 3Present address: Department of Ophthalmology, Baylor College of Medicine, Houston, Tex. 77030. ous system, eye, or genital tract of patients with a clinical diagnosis of HSV infection (results of testing of specimens from 12 of these patients were included in an earlier report [10]). Specimens were also collected from the same sites of patients with other diseases.…”

Section: Methodsmentioning

confidence: 99%

Diagnosis of herpes simplex virus infection by immunofluorescence

Taber¹,

F²,

Couch³

et al. 1976

J Clin Microbiol

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The utility of the indirect immunofluorescent antibody (IFA) technique for diagnosis of herpes simplex virus (HSV) infection was examined by testing specimens for this agent from 31 patients with encephalitis or meningitis, 17 with conjunctivitis, 19 with genital disease, and 1 with genital disease and meningitis. Brain biopsy tissue from four patients with encephalitis was positive by IFA and virus culture for HSV. Leukocytes in cerebrospinal fluid from these four patients and one with HSV meningitis were also positive by IFA, but virus isolation attempts on the fluid were all negative. Conjunctival scrapings from two patients with conjunctivitis were positive for HSV by both IFA and virus culture. Eleven of 12 culture-positive lesions of herpes progenitalis were positive by IFA, and 1 dark field-positive syphilitic chancre was also positive for HSV by both IFA and culture. Evidence for specificity of the results was provided by internal controls in each test and negative results from patients with other diagnoses. Thus, the IFA technique constituted a rapid, sensitive, and specific diagnostic method for the diagnosis of HSV infections.

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Section: Methodsmentioning

confidence: 99%

Diagnosis of herpes simplex virus infection by immunofluorescence

Taber¹,

F²,

Couch³

et al. 1976

J Clin Microbiol

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“…From 1965From up to 1970, out of thirty-nine cases where electron microscopy was performed, thirtysix showed the typical herpes-like particles (Rappel et al, 1971) and to date more than ten cases must be added to this series (Caulet et al, 1971;Laterre, 1972, Vital, 1972. Virus isolation and especially immunofluorescence techniques (Tomlinson & McCallum, 1969;Liu et al, 1971;Longson, 1973) have brought further evidence so that it can be assumedwith the possible exception of three cases (Duckett, Kelly & Grant, 1963;Scheid & Jochheim, 1956;Heathfield et al, 1967)-that ANE is of herpetic origin.…”

Section: Introductionmentioning

confidence: 99%

Management of acute necrotizing encephalitis: a review of 369 cases

Rappel¹

1973

Postgraduate Medical Journal

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Demonstration of herpes simplex virus DNA in CSF cells by in situ hybridization for early diagnosis of herpes encephalitis

Bamborschke

Porr²,

Huber

et al. 1990

J Neurol

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Herpes simplex virus (HSV) was studied by in situ DNA hybridization with a biotinylated cDNA probe in 56 air-dried methanol-fixed cerebrospinal fluid (CSF) cell preparations which had been collected from 12 patients with herpes simplex encephalitis (HSE) during the previous 5 years. In three additional HSE cases, freshly prepared acetone-fixed CSF cell preparations were available. In all cases, CSF cell preparations were obtained by cytocentrifugation. Herpes simplex virus DNA could be demonstrated in 8 of the 12 HSE cases with methanol-fixed cells (66%) and in all 3 cases with fresh acetone-fixed CSF cells. The earliest CSF sample was available at the onset of symptoms and showed positive DNA hybridization. In three cases hybridization was positive after a clinical course of more than 5 weeks but was usually found in the 1st week of illness before the beginning of specific inthrathecal IgG synthesis. In 54 control cases with other acute inflammatory diseases of the CNS, including 14 cases of varicella-zoster meningitis, no positive hybridization was detected. These findings strongly suggest that in situ hybridization in CSF cells is a reliable tool for the early and rapid diagnosis of HSE, especially at the onset of the disease, when no antibodies can be detected.

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Immunofluorescence in the diagnosis of herpes encephalitis

Cited by 7 publications

References 5 publications

Diagnosis of herpes simplex virus infection by immunofluorescence

Diagnosis of herpes simplex virus infection by immunofluorescence

Management of acute necrotizing encephalitis: a review of 369 cases

Demonstration of herpes simplex virus DNA in CSF cells by in situ hybridization for early diagnosis of herpes encephalitis

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