Rapid identification of Mycobacterium tuberculosis complex in clinical isolates by combining presumptive cord formation and MPT64 Antigen Immunochromatographic Assay

Kumar, Nishith; Agarwal, Ashwini; Tn, Dhole; Sharma, Yukti

doi:10.1016/j.ijtb.2015.04.007

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…In our laboratory we use combined presumptive cord formation and immunochromatographic test for detection of MPT 64 Antigen for identification of MTB complex as suggested in recently published studies. 17 These tests offer advantage of being rapid and are quite sensitive and specific when combined, 17 but further adds to the cost of primary isolation in manual MGIT.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of manual Mycobacterium growth indicator tube for isolation and susceptibility testing of Mycobacterium tuberculosis for implementation in low and medium volume laboratories

Agarwal

Kumar

Kishore

2018

Medical Journal Armed Forces India

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Section: Discussionmentioning

confidence: 99%

Evaluation of manual Mycobacterium growth indicator tube for isolation and susceptibility testing of Mycobacterium tuberculosis for implementation in low and medium volume laboratories

Agarwal

Kumar

Kishore

2018

Medical Journal Armed Forces India

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“…Detection of MPT64 and antigen 85B via immunostaining of tissues and fine-needle aspirates can be rapid and sensitive methods for establishing an early and specific diagnosis of TB infection but are limited to pathology and microbiology laboratories ( 42 , 43 ). Detection of MPT64 in sputum cultures utilizing an immune-chromatographic assay, in conjunction with cording in a stained smear, has been used to make a preliminary yet high-confidence identification of M. tuberculosis complex in liquid culture ( 44 , 45 ). Thus, localization of infection and the low bacterial burden in TB disease usually restricts the specimen type for direct detection methods to sputum or invasively acquired tissues.…”

Section: Discussionmentioning

confidence: 99%

Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications

et al. 2017

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Direct pathogen detection in blood to diagnose active tuberculosis (TB) has been difficult due to low levels of circulating antigens or due to the lack of specific, high-affinity binding reagents and reliable assays with adequate sensitivity. We sought to determine whether slow off-rate modified aptamer (SOMAmer) reagents with subnanomolar affinity for Mycobacterium tuberculosis proteins (antigens 85A, 85B, 85C, GroES, GroEL2, DnaK, CFP10, KAD, CFP2, RplL, and Tpx) could be useful to diagnose tuberculosis. When incorporated into the multiplexed, array-based proteomic SOMAscan assay, limits of detection reached the subpicomolar range in 40% serum. Binding to native M. tuberculosis proteins was confirmed by using M. tuberculosis culture filtrate proteins and fractions from infected macrophages and via affinity capture assays and subsequent mass spectrometry. Comparison of serum from culture-positive pulmonary TB patients and TB suspects systematically ruled out for TB revealed small but statistically significant (P < 0.0001) differences in the median M. tuberculosis signals and in specific pathogen markers, such as antigen 85B. Samples where many M. tuberculosis aptamers produced high signals were rare exceptions. In concentrated, protein-normalized urine from TB patients and non-TB controls, the CFP10 (EsxB) SOMAmer yielded the most significant differential signals (P < 0.0276), particularly in TB patients with HIV coinfection. In conclusion, direct M. tuberculosis antigen detection proved difficult even with a sensitive method such as SOMAscan, likely due to their very low, subpicomolar abundance. The observed differences between cases and controls had limited diagnostic utility in serum and urine, but further evaluation of M. tuberculosis SOMAmers using other platforms and sample types is warranted.

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“…MPT64, which is a 24-kDa protein of MTB and an important secretory protein of pathogenic bacteria, is often used as a candidate protein for diagnosis and in vaccines [6,7]. At present, there are many ways to detect the MPT64 protein, such as immunochromatography (ICT), ELISA, SD Bioline, and Capilia TB [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

MPT64 assays for the rapid detection of Mycobacterium tuberculosis

Wang

Zhou

et al. 2021

BMC Infect Dis

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Background Tuberculosis (TB) is a serious infectious disease caused by Mycobacterium tuberculosis (MTB). An estimated 1.7 billion people worldwide are infected with Mycobacterium tuberculosis (LTBI) during the incubation period without any obvious symptoms. Because of MTB’s high infection and mortality rates, there is an urgent need to develop a fast, portable, and sensitive diagnostic technology for its detection. Methods We included research from PubMed, Cochrane Library, Web of Science, and Embase and extracted the data. MetaDisc and STATA were used to build forest plots, Deek’s funnel plot, Fagan plot, and bivariate boxplot for analysis. Results Forty-six articles were analyzed, the results of which are as follows: sensitivity and specificity were 0.92 (0.91–0.93) and 0.95 (0.94–0.95) respectively. The NLR and PLR were 0.04 (95% CI 0.03–0.07) and 25.32 (95% CI 12.38–51.78) respectively. DOR was 639.60 (243.04–1683.18). The area under the SROC curve (AUC) was 0.99. Conclusions MPT64 exhibits good diagnostic efficiency for MTB. There is no obvious heterogeneity between the three commercial kits.

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Rapid identification of Mycobacterium tuberculosis complex in clinical isolates by combining presumptive cord formation and MPT64 Antigen Immunochromatographic Assay

Cited by 5 publications

References 12 publications

Evaluation of manual Mycobacterium growth indicator tube for isolation and susceptibility testing of Mycobacterium tuberculosis for implementation in low and medium volume laboratories

Evaluation of manual Mycobacterium growth indicator tube for isolation and susceptibility testing of Mycobacterium tuberculosis for implementation in low and medium volume laboratories

Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications

MPT64 assays for the rapid detection of Mycobacterium tuberculosis

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