MPT64 assays for the rapid detection of Mycobacterium tuberculosis

Abstract

Background Tuberculosis (TB) is a serious infectious disease caused by Mycobacterium tuberculosis (MTB). An estimated 1.7 billion people worldwide are infected with Mycobacterium tuberculosis (LTBI) during the incubation period without any obvious symptoms. Because of MTB’s high infection and mortality rates, there is an urgent need to develop a fast, portable, and sensitive diagnostic technology for its detection. Methods We included research from PubMed, Cochrane Library, Web of Science, and Embase and extracted the data. MetaDisc and STATA were used to build forest plots, Deek’s funnel plot, Fagan plot, and bivariate boxplot for analysis. Results Forty-six articles were analyzed, the results of which are as follows: sensitivity and specificity were 0.92 (0.91–0.93) and 0.95 (0.94–0.95) respectively. The NLR and PLR were 0.04 (95% CI 0.03–0.07) and 25.32 (95% CI 12.38–51.78) respectively. DOR was 639.60 (243.04–1683.18). The area under the SROC curve (AUC) was 0.99. Conclusions MPT64 exhibits good diagnostic efficiency for MTB. There is no obvious heterogeneity between the three commercial kits.

Differential Diagnosis of Latent Tuberculosis Infection and Active Tuberculosis: A Key to a Successful Tuberculosis Control Strategy

“…Previous studies have reported that the sensitivity and specificity of Rv3872 for detecting LTBI in children were 76% and 80%, respectively, which could elicit a stronger immunoreactivity for discriminating TB from HCs in patients who have received BCG vaccination (Mukherjee et al, 2007;Mahmoudi et al, 2017). In contrast, the remaining antigens (Rv3879, Rv1978, Rv1980c, Rv1981c, Rv3425, and Rv3429) were only tested between patients with TB and HCs with BCG vaccination or LTBI and HCs with BCG vaccination (Mukherjee et al, 2007;Zhang et al, 2007;Chen et al, 2009;Hinks et al, 2009;Wang et al, 2013;Luo et al, 2017;Mahmoudi et al, 2017;Cao et al, 2021). Hinks et al (2009) compared the responses to a range of RD1-encoded antigens (ESAT-6, CFP-10, Rv3879c, Rv3878, Rv3873, and Rv2031c) and defined a similar hierarchy of immunodominance for these antigens in both aTB and LTBI subjects: CFP-10 > ESAT-6 > Rv3879c > Rv3878 > Rv3873 > Rv2031c.…”

Rapid identification of Mycobacterium tuberculosis in cultures by molecular and immunological methods

“…It is noteworthy that, although the various steps of the protocol are important in DNA Regarding the MPT64 assays, these have already been shown to be efficient in the diagnosis of TB 5 . Compared to PCR, the MPT64 assay is easier to perform and does not require special instruments and advanced technical knowledge 5 . Moreover, it is faster and costs approximately US$ 1.4, for each test 15 .…”

“…These tests can identify MTBC isolates by detecting the 24 kDa MPT64 antigen, a protein secreted by viable MTBC cells. Immunological tests for detecting the MPT64 antigen (MPT64 assay) have the advantage of being simple to perform and release rapid and easy-to-interpret results 5,6 .…”

Osteoarticular tuberculosis cases in the southwest of China: A 9-year retrospective study

“…Among the 3,254 cases of osteoarticular TB infection, 2,261 (69.5%) were cases of spinal TB. The sites of spinal TB were divided into the cervical vertebrae (1-6), cervicothoracic region (cervical 7-thoracic 2), thoracic vertebrae (3)(4)(5)(6)(7)(8)(9)(10)(11), thoracolumbar region (thoracic 12, lumbar 1), lumbar vertebrae (2-4), lumbosacral region (lumbar 5, sacral 1), sacral vertebra, and coccyx. The results of statistical analysis showed that the lesions involved in spinal TB cases occurred in the lumbar (48%), thoracic (36%), sacral (6%), and cervical (2%) regions.…”