Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions.

Wirth, Dyann F.; Pratt, Diane McMahon

doi:10.1073/pnas.79.22.6999

Cited by 144 publications

(60 citation statements)

References 24 publications

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“…, 1987bNur, Leblanc and Tully, 1987;Razin etal., 1987;Roberts etal., 1987;Santha etal., 1987;Gobel et al. 1987), as well as bacteria (Wirth and Piatt, 1982;Fest!. Ludwig and Schleifer, 1986;Palva, 1986;Lazo, Roffey and Gabriel, 1987).…”

Section: Nucleic Acid Hybridization Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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Section: Nucleic Acid Hybridization Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…All membranes were initially floated on the surface of a hot (60°C) solution containing 0.5 N NaOH and 1.5 M NaCl for 30 s, removed, and incubated in the same solution at 22°C for 10 min (Roberts et al, 1985). The membranes were then transferred to a solution of 3 M Tris-HC1, pH 8.0, and incubated at room temperature for 10 min (Wirth and Pratt, 1982). After drying in air for 15 min, one membrane was baked for 1 h in vacuo at 80°C.…”

Section: Preparation Of Target Dnamentioning

confidence: 99%

Detection of Campylobacter pylori DNA by hybridisation with non-radioactive probes in comparison with a 32P-labelled probe

Wetherall

McDonald²,

Johnson

1988

Journal of Medical Microbiology

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Summary.A dot-blot hybridisation assay for the detection of Campylobacter pylori was used to compare a 32P-labelled probe with two biotinylated probes and a sulphonated probe. The minimum amount of pure C. pylori DNA that could be detected by the 32P-labelled probe was 100 pg, which corresponded to 5 x lo4 bacteria. A biotin-labelled DNA (biotin-DNA) probe together with the BluGeneTM detection system produced b Bethesda Research Laboratories (BRL), and a sulphonated probe nylon membranes could be used with both these non-radioactive detection systems. However, a photobiotin-labelled DNA (photobiotin-DNA) probe and detection system produced by Biotechnology Research Enterprises S.A. (BRESA) gave optimum results only with nitrocellulose membranes, and was quantitatively 100 times less sensitive than the other types of probe. The detection systems for the biotin-DNA and photobiotin-DNA probes produced non-specific reactions with crude bacterial blots of heterologous organisms ; these non-specific reactions could be removed by treating the dot blots with proteinase K, but not by treatment with RNAase. The sulphonated probe and detection system did not give any reaction with heterologous organism blots.and ChemiprobeT x detection system (Orgenics) gave similar levels of sensitivity;

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“…Las nuevas técnicas para la identificación "in situ" de los amastigotes de Leishmania usando anticuerpos monoclonales e hibridizacidn del k-DNA 23 -2 no son todavía aplicables en la investigación epidemiológica de campo.…”

Section: Introductionunclassified

El cultivo "in vitro" como instrumento práctico para el diagnóstico y aislamiento primario de Leishmania braziliensis braziliensis. 2. Estudios en pacientes de áreas endémicas

Cuba

Netto

Costa

et al. 1986

Rev. Inst. Med. trop. S. Paulo

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El cultivo "in vitro" de Leishmania braziliensis braziliensis constituye un método útil en el trabajo de campo, para el aislamiento primario de ésta subes-pécie de Leishmania. Cultivos en dos medios difásicos de agar sangre (DAB y EVANS) y dos medios líquidos (SCHNEIDER'S y AR-103) realizados en pacientes con lesiones cutáneas de Leishmaniasis Tegumentaria Americana (LTA) demostraron: 1) Similar sensibilidad de los medios DAB y Schneider's cuando utilizamos el procedimiento de aspiración de las muestras con aguja. 2) Rendimiento sensible y reproducible, con el medio DAB, cuando comparado, en repetidas ocasiones, con el medio EVANS. 3) Incremento significativo en el aislamiento primario de Leishmania braziliensis brazilensis mediante la ejecución, en la misma lesión de cada paciente, de tres aspiraciones consecutivas en sitios diferentes de la úlcera activa (50% de positividad, con el medio DAB).

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Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions.

Cited by 144 publications

References 24 publications

New Approaches to the Detection of Microbial Plant Pathogens

New Approaches to the Detection of Microbial Plant Pathogens

Detection of Campylobacter pylori DNA by hybridisation with non-radioactive probes in comparison with a 32P-labelled probe

El cultivo "in vitro" como instrumento práctico para el diagnóstico y aislamiento primario de Leishmania braziliensis braziliensis. 2. Estudios en pacientes de áreas endémicas

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