El cultivo "in vitro" como instrumento práctico para el diagnóstico y aislamiento primario de Leishmania braziliensis braziliensis. 2. Estudios en pacientes de áreas endémicas

Abstract

El cultivo "in vitro" de Leishmania braziliensis braziliensis constituye un método útil en el trabajo de campo, para el aislamiento primario de ésta subes-pécie de Leishmania. Cultivos en dos medios difásicos de agar sangre (DAB y EVANS) y dos medios líquidos (SCHNEIDER'S y AR-103) realizados en pacientes con lesiones cutáneas de Leishmaniasis Tegumentaria Americana (LTA) demostraron: 1) Similar sensibilidad de los medios DAB y Schneider's cuando utilizamos el procedimiento de aspiración de las muestras con aguja. 2) Rendimiento sensible y reproducible, con el medio DAB, cuando comparado, en repetidas ocasiones, con el medio EVANS. 3) Incremento significativo en el aislamiento primario de Leishmania braziliensis brazilensis mediante la ejecución, en la misma lesión de cada paciente, de tres aspiraciones consecutivas en sitios diferentes de la úlcera activa (50% de positividad, con el medio DAB).

South American cutaneous Leishmaniasis of the eyelids

“…The second part was used for cultivation in NNN media. 5 When possible, the culture was identified according to species by the isoenzyme technique. 6…”

Chronic Aerobic Training at Different Volumes in the Modulation of Macrophage Function and in vivo Infection of BALB/c Mice by Leishmania major

“…These tissues were macerated in Schneider's medium, added with streptomycin/penicillin 0.1%. The result of the maceration was inoculated into 15 ml screw cap tubes, containing 5.0 ml of Schneider's medium, supplemented with 20% fetal bovine serum and blood agar, and kept at 26 • C. After isolation, the parasites were maintained under previously described conditions (Cuba et al, 1986) in Schneider's medium supplemented with 2 mM glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin, and 10% fetal calf serum in a humidified incubator at 26 • C. The parasites were cryopreserved, just once, soon after isolation (P1) and kept in liquid nitrogen until the beginning of this work. Subsequently, they were thawed and expanded in vitro to carry out the infection experiments being, at most, in the fourth passage (P4) during the performance of these experiments.…”