Rapid identification of HPV 16 and 18 by multiplex nested PCR-immunochromatographic test

Kuo, Yung-Bin; Li, Yi-Shuan; Chan, Err–Cheng

doi:10.1016/j.jviromet.2014.10.009

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…PCR is a standard test method for HPV, which has the advantage of detecting specific HPV types and detecting viral load using PCR technology. Its sensitivity is high and can detect less than 10 copies of HPV genomic DNA (13). ISH has high sensitivity and specificity, usually used for tissue sectioning or cell smear examination, which can provide not only the precise positioning of the target sequence but also the details of tissue or cell morphology (14).…”

Section: Discussionmentioning

confidence: 99%

The correlation between infections by human papillomavirus types 6/11 and 16/18 and mammary gland hyperplasia with glandular thickening

Deng

Long

2019

Cell Mol Biol (Noisy-le-grand)

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The aim of the present study was to investigate the correlation between human papillomavirus (HPV) type 6/11 and 16/18 infections and glandular thickening mammary gland hyperplasia in order to explore methods for preventing glandular thickening mammary gland hyperplasia. A total of 240 patients with glandular thickening mammary gland hyperplasia who were treated by surgery in our hospital from January 2012 to June 2017 were enrolled in the present study.The hyperplastic breast tissue and adjacent normal breast tissue were taken to test HPV type 6/11 and 16/18 infections using conventional PCR and in situ hybridization techniques. The correlations between HPV type 6/11 and 16/18 infections and glandular thickening mammary gland hyperplasia were analyzed using statistical methods of chi-square test. The infection rates of HPV type 6/11 and 16/18 in the hyperplastic breast tissue were 31.95% and 34.91%, respectively and 11.83% and 14.79% in the normal breast tissue, respectively. The differences were statistically significant (all p<0.05). HPV type 6/11 and 16/18 infections may be closely related to the development of glandular thickening mammary gland hyperplasia, and may be one of the causes of glandular thickening mammary gland hyperplasia.

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Section: Discussionmentioning

confidence: 99%

The correlation between infections by human papillomavirus types 6/11 and 16/18 and mammary gland hyperplasia with glandular thickening

Deng

Long

2019

Cell Mol Biol (Noisy-le-grand)

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“…In recent years, new ICTS-based detection methods have emerged, which means that ICTS methods are practical in many fields. These methods reduce the detection workload and the possibility of bacterial contamination ( Kuo et al, 2015 ; Vyas et al, 2015 ). The ICTS method, combined with some special equipment, can process large-volume samples in complex analytical processes.…”

Section: Discussionmentioning

confidence: 99%

Rapid quantitative detection of Klebsiella pneumoniae in infants with severe infection disease by point-of-care immunochromatographic technique based on nanofluorescent microspheres

Chen

Sha²,

Li³

et al. 2023

Front. Bioeng. Biotechnol.

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Background:Klebsiella pneumoniae (KP, K. pneumoniae) is one of the most important nosocomial pathogens that cause severe respiratory infections. As evolutionary high-toxic strains with drug resistance genes increase year by year, the infections caused by it are often accompanied by high mortality, which may be fatal to infants and can cause invasive infections in healthy adults. At present, the traditional clinical methods for detecting K. pneumoniae are cumbersome and time-consuming, and the accuracy and sensitivity are not high. In this study, nanofluorescent microsphere (nFM)-based immunochromatographic test strip (ICTS) quantitative testing platform were developed for point-of-care testing (POCT) method of K. pneumoniae.Methods: 19 clinical samples of infants were collected, the genus-specific gene of mdh was screened from K. pneumoniae. Polymerase chain reaction (PCR) combined with nFM-ICTS based on magnetic purification assay (PCR-ICTS) and strand exchange amplification (SEA) combined with nFM-ICTS based on magnetic purification assay (SEA-ICTS) were developed for the quantitative detection of K. pneumoniae. The sensitivity and specificity of SEA-ICTS and PCR-ICTS were demonstrated by the existing used classical microbiological methods, the real-time fluorescent quantitative PCR (RTFQ-PCR) and PCR assay based on agarose gel electrophoresis (PCR-GE).Results: Under optimum working conditions, the detection limits of PCR-GE, RTFQ-PCR, PCR-ICTS and SEA-ICTS are 7.7 × 10−3, 2.5 × 10−6, 7.7 × 10−6, 2.82 × 10−7 ng/μL, respectively. The SEA-ICTS and PCR-ICTS assays can quickly identify K. pneumoniae, and could specifically distinguish K. pneumoniae samples from non-K. pneumoniae samples. Experiments have shown a diagnostic agreement of 100% between immunochromatographic test strip methods and the traditional clinical methods on the detection of clinical samples. During the purification process, the Silicon coated magnetic nanoparticles (Si-MNPs) were used to removed false positive results effectively from the products, which showed of great screening ability. The SEA-ICTS method was developed based on PCR-ICTS, which is a more rapid (20 min), low-costed method compared with PCR-ICTS assay for the detection of K. pneumoniae in infants. Only need a cheap thermostatic water bath and takes a short detection time, this new method can potentially serve as an efficient point-of-care testing method for on-site detection of pathogens and disease outbreaks without fluorescent polymerase chain reaction instruments and professional technicians operation.

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“…In yet another approach, a combination of isothermal recombinase polymerase amplification (RPA), reverse dot-blot assay (RDB), and lateral flow dipstick (LFD) has been designed to identify various genotypes of HPV viral DNA. [64][65][66][67][68] To reduce the time, cost, and requirement of expensive instruments to conduct PCR, interesting strategies using CRISPR-Cas systems are gaining importance. Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that employs 2 components: a single guide RNA to detect the target DNA sequence and a CRISPR-associated protein (Cas) endonuclease to cleave the target sequence upon homology recognition.…”

Section: Naat-ica/naat-lfamentioning

confidence: 99%

Paper-based point of care diagnostics for cancer biomarkers

Bhardwaj,

Arora,

Saxena

et al. 2024

Sens. Diagn.

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Rapid identification of HPV 16 and 18 by multiplex nested PCR-immunochromatographic test

Cited by 8 publications

References 17 publications

The correlation between infections by human papillomavirus types 6/11 and 16/18 and mammary gland hyperplasia with glandular thickening

The correlation between infections by human papillomavirus types 6/11 and 16/18 and mammary gland hyperplasia with glandular thickening

Rapid quantitative detection of Klebsiella pneumoniae in infants with severe infection disease by point-of-care immunochromatographic technique based on nanofluorescent microspheres

Paper-based point of care diagnostics for cancer biomarkers

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