Rapid identification of diverse Campylobacter lari strains isolated from mussels and oysters using a reverse hybridization line probe assay

Doorn, Leen Jan Van; Haperen, Anita Verschuuren-van; Belkum, Alex van; Endtz, Hubert P.; Vliegenthart, J. S.; Vandamme, Peter; Quint, Wim

doi:10.1046/j.1365-2672.1998.00378.x

Cited by 29 publications

(13 citation statements)

References 32 publications

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“…These results indicate that the UPTC phenotype is not confined to a single taxon and that it is a variable characteristic within the taxon. The AFLP patterns of UPTC strains were extremely heterogeneous and confirmed the previously observed variations in randomly amplified polymorphic DNA patterns and GTPase gene sequences of these strains (11,32). The previously described indistinguishable pulsed-field gel electrophoresis patterns obtained for three UPTC strains from seagulls are inconsistent with these results (17), although this finding may have been affected by sampling bias and the low number of strains tested.…”

Section: Discussioncontrasting

confidence: 46%

Identification of Distinct Campylobacter lari Genogroups by Amplified Fragment Length Polymorphism and Protein Electrophoretic Profiles

Duim¹,

Wagenaar²,

Dijkstra³

et al. 2004

Appl Environ Microbiol

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Campylobacter lari is a phenotypically and genotypically diverse species that comprises the classical nalidixic acid-resistant thermophilic campylobacters (NARTC) and the biochemical C. lari variants, including the urease-positive campylobacters (UPTC), the nalidixic acid-susceptible campylobacters (NASC), and the urease-producing nalidixic acid-susceptible campylobacters. To study the taxonomic and epidemiological relationships among strains of the C. lari variants, amplified fragment length polymorphism (AFLP) profiling and whole-cell protein profile analysis were performed with 55 C. lari strains. Great genetic heterogeneity in AFLP and protein profiles was observed. Numerical analysis of AFLP profiles and of partial protein profiles allowed discrimination of four distinct genogroups. AFLP cluster I included nearly homogeneous patterns for C. lari NARTC strains (genogroup I). UPTC strains together with non-urease-producing NASC strains produced highly diverse patterns and were placed in genogroup II. The genogroup III strains had the NASC phenotype and produced more homogeneous patterns. Finally, genogroup IV strains had the classical NARTC phenotype and produced AFLP patterns that were very distinct from those of other genogroups. One UPTC strain had aberrant patterns and clustered separately, which may indicate that there is an additional genogroup. Preliminary DNA-DNA hybridization experiments suggested that genogroups I and III represent a single genomic species and that genogroup IV represents a distinct species. The detection of moderate levels of DNA-DNA hybridization between a genogroup II reference strain and genogroup I and III reference strains highlights the need for further DNA-DNA hybridization experiments to clarify the taxonomic status of the former group. No correlation of genogroups with different sources of strains was identified. These data show that UPTC strains are genetically diverse and distinct from NARTC strains. In addition, they indicate that the classical NARTC phenotype encompasses at least two genogroups.

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Section: Discussioncontrasting

confidence: 46%

Identification of Distinct Campylobacter lari Genogroups by Amplified Fragment Length Polymorphism and Protein Electrophoretic Profiles

Duim¹,

Wagenaar²,

Dijkstra³

et al. 2004

Appl Environ Microbiol

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“…nov. to encompass the classical nalidixic-acid-resistant, urease-negative isolates typified by the type strain. The divergence of the novel taxa presented here has been confirmed in previous studies, including a multilocus sequence typing system analysis (Miller et al, 2005) and a phylogenetic analysis of a partial GTPaseencoding gene (van Doorn et al, 1998). Benjamin et al 1984 Cells are Gram-negative, spiral to curved rods, 0.361.7-2.4 mm in size.…”

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confidence: 58%

Novel Campylobacter lari-like bacteria from humans and molluscs: description of Campylobacter peloridis sp. nov., Campylobacter lari subsp. concheus subsp. nov. and Campylobacter lari subsp. lari subsp. nov.

Debruyne

Brandt

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Campylobacter jejuni and Campylobacter coli are particularly frequent causative agents of human bacterial intestinal disorders worldwide (Skirrow, 1994). There have also been reported cases of diarrhoea in man caused by Campylobacter upsaliensis and Campylobacter lari, but the frequency of these infections is very low (Bourke et al, 1998;Van Doorn et al, 1998). Occasionally, Campylobacter species are implicated as causative agents of pericarditis, myocarditis and Guillain-Barré Syndrome (Kuwabara, 2004;Uzoigwe, 2005).…”

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confidence: 99%

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Korczak

Stieber

Emler

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.The genus Campylobacter was first proposed by M. Sebald and M. Véron in 1963 and included only the type species Campylobacter fetus and Campylobacter bubulus, now known as Campylobacter sputorum (Sebald & Véron, 1963). These taxa had formerly been classified as Vibrio species. In 1973, M. Véron and R. Chatelain included several misclassified Vibrio in the distinct Campylobacter genus based on serological, biochemical and DNA-DNA hybridization investigations (On, 2001). Since then, the taxonomy of the genus has changed dramatically. At present, it comprises 17 species with validly published names and six recognized subspecies (On, 2001;Foster et al., 2004). In addition, strains belonging to C. sputorum are divided into three biovars, sputorum, fecalis and paraureolyticus, on the basis of their ability to produce catalase or urease Vandamme & On, 2001).In general, members of the genus Campylobacter colonize the mucosal surfaces of the intestinal tract, oral cavity or urogenital tract of healthy, as well as diseased, humans and animals, especially birds. Several species may act as pathogens, causing disease in both human and animal hosts. Twelve of the 17 Campylobacter species are associated with human diseases. Campylobacter jejuni and Campylobacter coli are particularly frequent causative agents of human bacterial intestinal disorders worldwide (Skirrow, 1994). There have also been reported cases of diarrhoea in man caused by Campylobacter upsaliensis and Campylobacter lari, but the frequency of these infections is very low (Bourke et al., 1998;Van Doorn et al., 1998). Occasionally, Campylobacter species are implicated as causative agents of pericard...

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Rapid identification of diverse Campylobacter lari strains isolated from mussels and oysters using a reverse hybridization line probe assay

Cited by 29 publications

References 32 publications

Identification of Distinct Campylobacter lari Genogroups by Amplified Fragment Length Polymorphism and Protein Electrophoretic Profiles

Identification of Distinct Campylobacter lari Genogroups by Amplified Fragment Length Polymorphism and Protein Electrophoretic Profiles

Novel Campylobacter lari-like bacteria from humans and molluscs: description of Campylobacter peloridis sp. nov., Campylobacter lari subsp. concheus subsp. nov. and Campylobacter lari subsp. lari subsp. nov.

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

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