Identification of Distinct
            <i>Campylobacter lari</i>
            Genogroups by Amplified Fragment Length Polymorphism and Protein Electrophoretic Profiles

Duim, Birgitta; Wagenaar, Jaap A.; Dijkstra, Jeroen R.; Goris, Johan; Endtz, Hubert P.; Vandamme, Peter

doi:10.1128/aem.70.1.18-24.2004

Cited by 29 publications

(19 citation statements)

References 33 publications

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“…Campylobacter lari is a phenotypically and genetically diverse species, and variants include nalidixic acid‐resistant (NAR), nalidixic acid‐susceptible (NASC), urease‐positive and urease‐negative strains. Genetic heterogeneity is also observed within this group, and AFLP (amplified fragment length polymorphism) (Duim et al. 2004) and gene sequence analysis (Korczak et al.…”

Section: Discussionmentioning

confidence: 99%

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The occurrence of Campylobacter in river water and waterfowl within a watershed in southern Ontario, Canada

Dyke

Morton

McLellan

et al. 2010

Journal of Applied Microbiology

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Aims: Quantitative PCR and a culture method were used to investigate Campylobacter occurrence over 3 years in a watershed located in southern Ontario, Canada that is used as a source of drinking water. Methods and Results: Direct DNA extraction from river water followed by quantitative PCR analysis detected thermophilic campylobacters at low concentrations (<130 cells 100 ml−1) in 57–79% of samples taken from five locations. By comparison, a culture‐based method detected Campylobacter in 0–23% of samples. Water quality parameters such as total Escherichia coli were not highly correlated with Campylobacter levels, although higher pathogen concentrations were observed at colder water temperatures (<10°C). Strains isolated from river water were primarily nalidixic acid‐susceptible Campylobacter lari, and selected isolates were identified as Campylobacter lari ssp. concheus. Campylobacter from wild birds (seagulls, ducks and geese) were detected at a similar rate using PCR (32%) and culture‐based (29%) methods, and although Campylobacter jejuni was isolated most frequently, C. lari ssp. concheus was also detected. Conclusions: Campylobacter were frequently detected at low concentrations in the watershed. Higher prevalence rates using quantitative PCR was likely because of the formation of viable but nonculturable cells and low recovery of the culture method. In addition to animal and human waste, waterfowl can be an important contributor of Campylobacter in the environment. Significance and Impact of the Study: Results of this study show that Campylobacter in surface water can be an important vector for human disease transmission and that method selection is important in determining pathogen occurrence in a water environment.

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Section: Discussionmentioning

confidence: 99%

“…2006) could differentiate numerous subtypes within this species. However, the clinical significance of the C. lari subtypes is not understood (Duim et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

The occurrence of Campylobacter in river water and waterfowl within a watershed in southern Ontario, Canada

Dyke

Morton

McLellan

et al. 2010

Journal of Applied Microbiology

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“…2007). In another study, taxonomic and epidemiological relationships among 55 strains of Camphylobacter lari , were investigated using whole cell protein profiles and amplified fragment length polymorphism (AFLP) (Duim et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

Whole cell protein profiling reiterate phylogenetic relationships among strains of Yersinia enterocolitica biovar 1A as discerned earlier by different genotyping methods

Mallik

Virdi

2010

Journal of Applied Microbiology

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Aim: To evaluate whole cell protein profiling vis‐à‐vis genotyping to discern phylogenetic relationships among strains of Y. enterocolitica biovar 1A. Methods and Results: Whole cell protein profiling of Y. enterocolitica biovar 1A strains was performed using SDS–PAGE. Twenty‐one distinct protein profile types were obtained among a collection of 81 strains isolated from clinical and nonclinical sources. Whole cell protein profiling exhibited discriminatory index (DI) of 0·80 and clustered the strains into two distinct clonal groups. The clinical and the aquatic serotype O:6,30–6,31 strains were clustered into two discrete subgroups. Conclusions: Whole cell protein profiling displayed sufficient diversity among strains of Y. enterocolitica biovar 1A, and the phylogenetic relationships obtained were in good agreement with those established earlier by genotyping techniques. Significance and impact of the study: Whole cell protein profiling was as discriminatory as some of the genotyping methods and has the potentiality to be used as an adjunct tool to study epidemiology of Y. enterocolitica.

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“…have been classified as C. lari (3). Several investigators have found that isolates within the C. lari taxon are very heterogeneous (8,25). With multilocus enzyme electrophoresis, Matsuda et al (25) showed that urease-positive thermophilic campylobacters are genetically hypervariable and form a cluster separate from the main C. lari cluster, while Duim et al (8) used fragment length polymorphism, whole-cell protein profiles, and limited DNA-DNA hybridization analyses to reach a similar conclusion.…”

Section: Vol 42 2004mentioning

confidence: 98%

Differentiation of Campylobacter coli , Campylobacter jejuni , Campylobacter lari , and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA

et al. 2004

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We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in Escherichia coli strain SM105 [lpxA(Ts)]. With oligonucleotide primers developed to the C. jejuni lpxA gene, nearly full-length lpxA amplicons were amplified from an additional 11 isolates of C. jejuni, 20 isolates of C. coli, 16 isolates of C. lari, and five isolates of C. upsaliensis. The nucleotide sequence of each amplicon was determined, and sequence alignment revealed a high level of species discrimination. Oligonucleotide primers were constructed to exploit species differences, and a multiplex PCR assay was developed to positively identify isolates of C. coli, C. jejuni, C. lari, and C. upsaliensis. We characterized an additional set of 41 thermotolerant isolates by partial nucleotide sequence analysis to further demonstrate the uniqueness of each species-specific region. The multiplex PCR assay was validated with 105 genetically defined isolates of C. coli, C. jejuni, C. lari, and C. upsaliensis, 34 strains representing 12 additional Campylobacter species, and 24 strains representing 19 non-Campylobacter species. Application of the multiplex PCR method to whole-cell lysates obtained from 108 clinical and environmental thermotolerant Campylobacter isolates resulted in 100% correlation with biochemical typing methods.

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Identification of Distinct Campylobacter lari Genogroups by Amplified Fragment Length Polymorphism and Protein Electrophoretic Profiles

Cited by 29 publications

References 33 publications

The occurrence of Campylobacter in river water and waterfowl within a watershed in southern Ontario, Canada

The occurrence of Campylobacter in river water and waterfowl within a watershed in southern Ontario, Canada

Whole cell protein profiling reiterate phylogenetic relationships among strains of Yersinia enterocolitica biovar 1A as discerned earlier by different genotyping methods

Differentiation of Campylobacter coli , Campylobacter jejuni , Campylobacter lari , and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA

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