Whole cell protein profiling reiterate phylogenetic relationships among strains of Yersinia enterocolitica biovar 1A as discerned earlier by different genotyping methods

Abstract

Aim: To evaluate whole cell protein profiling vis‐à‐vis genotyping to discern phylogenetic relationships among strains of Y. enterocolitica biovar 1A. Methods and Results: Whole cell protein profiling of Y. enterocolitica biovar 1A strains was performed using SDS–PAGE. Twenty‐one distinct protein profile types were obtained among a collection of 81 strains isolated from clinical and nonclinical sources. Whole cell protein profiling exhibited discriminatory index (DI) of 0·80 and clustered the strains into two distinct clonal groups. The clinical and the aquatic serotype O:6,30–6,31 strains were clustered into two discrete subgroups. Conclusions: Whole cell protein profiling displayed sufficient diversity among strains of Y. enterocolitica biovar 1A, and the phylogenetic relationships obtained were in good agreement with those established earlier by genotyping techniques. Significance and impact of the study: Whole cell protein profiling was as discriminatory as some of the genotyping methods and has the potentiality to be used as an adjunct tool to study epidemiology of Y. enterocolitica.

Genotypic diversity and virulence markers ofYersinia enterocoliticabiotype 1A strains isolated from clinical and non-clinical origins

“…Moreover, the presence of the gene hreP was correlated with the presence of ystB . Our study corroborates with studies where B1A strains isolated from India, USA, French, Germany and Finland were divided into clusters that differed in the frequency of some virulence markers .…”

“…Moreover, these studies showed that the presence of certain virulence genes was related to a specific cluster and not to the clinical or non‐clinical sources of isolation. These studies were conducted with strains isolated mainly from India, Finland, as well as, a few strains isolated in France, Germany and the United States .…”

Characteristics of Yersinia enterocolitica biotype 1A strains isolated from patients and asymptomatic carriers

“…Two major clonal groups of Y. enterocolitica 1A have been reported earlier by different genotypic and phenotypic methods [10,11,17,22]. Furthermore, Bhagat and Virdi have shown a correlation between the distribution of virulence-associated genes myfA, ystB and 10 hreP and the clonal groups [3].…”