Rapid Identification of Dengue Virus Isolates by using Monoclonal Antibodies in an Indirect Immunofluorescence Assay

Henchal, Erik A.; McCown, Jack M.; Seguin, M C; Gentry, Mary K.; Brandt, Walter E.

doi:10.4269/ajtmh.1983.32.164

Cited by 133 publications

(88 citation statements)

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“…Sera that have been collected from suspected dengue cases in the first 3-5 days of fever (the viraemic phase) can be used for virus isolation. After an incubation period permitting virus replication, viral identification is performed using dengue-specific monoclonal antibodies in immunofluorescence and PCR assays 63,64,72,73 . Serum is often used for virus isolation but plasma, leukocytes, whole blood and tissues obtained at autopsy can also be used 63,74,75 .…”

Section: Virus Isolationmentioning

confidence: 99%

Dengue: a continuing global threat

et al. 2010

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Dengue fever and dengue haemorrhagic fever are important arthropod-borne viral diseases. Each year, there are ~50 million dengue infections and ~500,000 individuals are hospitalized with dengue haemorrhagic fever, mainly in Southeast Asia, the Pacific and the Americas. Illness is produced by any of the four dengue virus serotypes. A global strategy aimed at increasing the capacity for surveillance and outbreak response, changing behaviours and reducing the disease burden using integrated vector management in conjunction with early and accurate diagnosis has been advocated. Antiviral drugs and vaccines that are currently under development could also make an important contribution to dengue control in the future.Dengue is the most important arthropod-borne viral infection of humans. Worldwide, an estimated 2.5 billion people are at risk of infection, approximately 975 million of whom live in urban areas in tropical and sub-tropical countries in Southeast Asia, the Pacific and the Americas 1 . Transmission also occurs in Africa and the Eastern Mediterranean, and rural Europe PMC Funders GroupAuthor Manuscript Nat Rev Microbiol. Author manuscript; available in PMC 2015 February 19. Published in final edited form as:Nat Rev Microbiol. 2010 December ; 8(12 0): S7-16. doi:10.1038/nrmicro2460. Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts communities are increasingly being affected. It is estimated that more than 50 million infections occur each year, including 500,000 hospitalizations for dengue haemorrhagic fever, mainly among children, with the case fatality rate exceeding 5% in some areas [1][2][3][4] . The geographical areas in which dengue transmission occurs have expanded in recent years (FIG. 1), and all four dengue virus serotypes (DENV-1-4) are now circulating in Asia, Africa and the Americas, a dramatically different scenario from that which prevailed 20 or 30 years ago (FIG. 2). The molecular epidemiology of these serotypes has been studied in an attempt to understand their evolutionary relationships 11 .This Review will provide an update on our understanding of the pathogenesis of this successful pathogen, how we diagnose and control infection and the progress that has been made in vaccine development. Dengue virus pathogenesisDengue viruses belong to the genus flavivirus within the Flaviviridae family. DENV-1-4 evolved in non-human primates from a common ancestor and each entered the urban cycle independently an estimated 500-1,000 years ago 12 . The virion comprises a spherical particle, 40-50 nm in diameter, with a lipopolysaccharide envelope. The positive singlestrand RNA genome (FIG. 3), which is approximately 11 kb in length, has a single open reading frame that encodes three structural proteins -the capsid (C), membrane (M) and envelope (E) glycoproteins -and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5 [18][19][20] . ADE occurs when mononuclear phagocytes are infected through their Fc receptors by immune complexes that form between DE...

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Section: Virus Isolationmentioning

confidence: 99%

Dengue: a continuing global threat

et al. 2010

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“…The method of choice for dengue virus identification is IFA with serotype-specific monoclonal antibodies produced in tissue culture or mouse ascitic fluids and an anti-mouse immunoglobulin G-fluorescein isothiocyanate conjugate (47,52,55,72). This test can be easily performed with infected cell cultures, mosquito brain or tissue squashes, mouse brain squashes, or even on formalin-fixed tissues embedded in par-affin and sectioned for histopathologic testing (56).…”

Section: Virus Identificationmentioning

confidence: 99%

Dengue and Dengue Hemorrhagic Fever

Gubler¹

1997

Tropical Infectious Diseases

604

756

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“…The use of serotype-specific monoclonal antibodies and the reverse transcriptase-polymerase chain reaction (RT-PCR) for virus identification during routine virologic surveillance allowed detection of concurrent infections for the first time. [4][5][6] The first case of a dual infection with 2 dengue virus serotypes was reported in Puerto Rico in 1982; dengue 1 (DEN-1) and DEN-4 circulated in the population during that time, and both virus serotypes were isolated from a single patient with dengue fever. 7 In New Caledonia, DEN-1 and DEN-3 viruses were isolated from 6 patients with DF in 1989.…”

mentioning

confidence: 99%

Common occurrence of concurrent infections by multiple dengue virus serotypes.

Loroño-Pino

Cropp²,

Farfan³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

126

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Abstract. The co-circulation of all 4 dengue virus serotypes in the same community, common since the 1950s in Southeast Asia, has now become a frequent occurrence in many Caribbean Islands, Mexico, and Central and South America in the past 20 years. As a consequence, the frequency of concurrent infections would be expected to increase in these areas. To assess this, using state of the art technology, we screened viremic serum samples and mosquitoes inoculated with serum samples collected during epidemics involving multiple dengue virus serotypes in Indonesia, Mexico, and Puerto Rico for virus isolation. Of 292 samples tested, 16 (5.5%) were found to contain 2 or more dengue viruses by an indirect immunofluorescence test and/or the reverse transcriptase-polymerase chain reaction.The global epidemiology of dengue/dengue hemorrhagic fever (DF/DHF) has changed dramatically in the past 50 years, first in Southeast Asia during and following World War II, and in the past 20 years in other tropical regions of the world, especially in the Americas.

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Rapid Identification of Dengue Virus Isolates by using Monoclonal Antibodies in an Indirect Immunofluorescence Assay

Cited by 133 publications

References 0 publications

Dengue: a continuing global threat

Dengue: a continuing global threat

Dengue and Dengue Hemorrhagic Fever

Common occurrence of concurrent infections by multiple dengue virus serotypes.

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