Common occurrence of concurrent infections by multiple dengue virus serotypes.

Loroño-Pino, María Alba; Cropp, C B; Farfan, Jose A.; Vorndam, A. Vance; Rodríguez-Angulo, Elsa; Rosado-Paredes, Elsy P.; Flores-Flores, Luis F.; Beaty, Barry J.; Gubler, D. J.

doi:10.4269/ajtmh.1999.61.725

Cited by 128 publications

(112 citation statements)

References 23 publications

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“…Another important findings of this study was that people exposed to DENV had a significantly higher SGEIgG antibody concentration. These results support the assumption that the risk of virus transmission increases in DENV-endemic areas where people may be exposed to several DENV serotypes in a single infection (29,30). A recent retrospective study showed that the IgG response to the Ae.…”

Section: Discussionsupporting

confidence: 75%

Concentración de los anticuerpos en contra de proteínas de las glándulas salivares de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

Londoño-Rentería

Cardenas²,

Giovanni

et al. 2015

biomedica

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Introduction:Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology. Objective: To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area. Materials and methods: A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENVIgG antibodies (DENV-IgG), in addition to IgG and IgM anti-Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper. Results: We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE. Conclusion: Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations.

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Section: Discussionsupporting

confidence: 75%

Concentración de los anticuerpos en contra de proteínas de las glándulas salivares de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

Londoño-Rentería

Cardenas²,

Giovanni

et al. 2015

biomedica

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“…Cocirculation of multiple DENV serotypes would result in an increased risk of concurrent infections. There is, however, limited documentation describing concurrent infections with more than one serotype in the same individual [13,14]. Furthermore, as already alluded to, sequential infection with more than one [15].…”

Section: Comparison Of the Significant Differences In Clinical Presenmentioning

confidence: 99%

“…All the patients survived during 2005, but 4.55%, 25%, and 2.7% of patients died during 2006-2008. Therefore, the highest mortality rate (25%) occurred during 2007, when 25% of patients had Hb levels < 5g/dL, and 50% had severe thrombocytopenia (11)(12)(13)(14)(15)(16)(17)(18)(19)(20),000 platelets/μL). Consequently, 25% of patients required blood and platelet transfusions.…”

Section: Comparison Of the Significant Differences In Clinical Presenmentioning

confidence: 99%

Clinical profile of dengue fever infection in King Abdul Aziz University Hospital Saudi Arabia

Ahmed

2010

J Infect Dev Ctries

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Background: The study aimed to compare the clinical profile of all patients diagnosed with dengue viral infection at King Abdul Aziz University Hospital (KAAUH), during [2005][2006][2007][2008]. Methodology: This retrospective study included 147 patients infected with dengue virus, age ≤ 16 years. Laboratory and haematological data were included. Results: Two peaks of infection occurred during 2006 and another two in 2008. Common clinical symptoms were fever, vomiting, and abdominal pain. Common haematological abnormalities were thrombocytopenia and leucopoenia. Differences existed between the years in the percentage of patients with fever, elevated alanine aminotransferase (ALT), direct bilirubin, lactate dehydrogenase (LDH), fibrin degradation products (FDPs), and haemoglobin (Hb) levels. Differences were found in nationalities between the years, but patient nationality had no effect on disease incidence. Differences were noted in the percentages of patients' immunoglobulin M (IgM) and polymerase chain reaction (PCR) positive. There was a slight inverse correlation of IgM positive with patient age. PCR, fever, ALT, direct bilirubin, LDH, FDPs, Hb, blood transfusion, and platelet transfusion showed no correlation with age or nationality. In 2005, all the patients survived, but there were 4.55%, 25%, and 2.7% deaths during 2006-2008. Conclusions: Significant differences in the clinical presentation of dengue virus (DENV) infection, indicative of a variation in disease severity from dengue fever (DF) to dengue hemhorrhagic fever (DHF)/dengue shock syndrome (DSS), were noted over the years. Possible reasons are infection with different serotypes, concurrent/sequential infection of more than one serotype, and differences in host immune responses associated with host genetic variations.

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“…Concurrent multiserotype infections by DENV have been described in several dengue-prevalent countries [7,8,10,[15][16][17]. The proportion of such patients could be as high as 5%-15% [16,17], and the number of serotypes as many as 3 [7,8,15].…”

Section: Discussionmentioning

confidence: 99%

Time course of concurrent infection with dengue virus serotypes 2 and 4 detected in urine

et al. 2015

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Background:In hyperendemic areas, concurrent multiserotype dengue virus (DENV) infections commonly occur in both humans and in mosquito vectors. Previous studies have focused on single blood specimens. Objectives: To illustrate and characterize the time course of mixed infection with DENV serotypes 2 (DENV2) and 4 (DENV4) in a single case. Materials and methods: Plasma, saliva, and urine were collected from a patient diagnosed with dengue hemorrhagic fever grade II and secondary DENV infection on days 7, 18, and 31 of his illness. DENV RNA detection was performed using 2 DENV-specific reverse transcriptase-polymerase chain reaction protocols. Cloning and sequencing were performed to quantify the major and minor viral populations in dual-serotype-infected specimen(s). Genotypes of both DENV serotypes were characterized. Results: DENV genome was detected in plasma and saliva only at the first time point (day 7 of illness), but in urine at both the first and second time points (days 7 and 18 of illness). DENV4 serotype was found in all DENV genome-positive specimens. DENV2 serotype was unexpectedly also detected in the first urine specimen. DENV4 as the major and DENV2 as the minor population. The DENV2 serotype was clustered in genotype Asian I and DENV4 serotype in genotype I. Conclusion: To our knowledge, this is the first report of concurrent multiserotype DENV infection detected solely in urine. Prospective noninvasive investigations may determine the prevalence of this phenomenon. Clinical and public health implications of this finding need to be explored.

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Common occurrence of concurrent infections by multiple dengue virus serotypes.

Cited by 128 publications

References 23 publications

Concentración de los anticuerpos en contra de proteínas de las glándulas salivares de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

Concentración de los anticuerpos en contra de proteínas de las glándulas salivares de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

Clinical profile of dengue fever infection in King Abdul Aziz University Hospital Saudi Arabia

Time course of concurrent infection with dengue virus serotypes 2 and 4 detected in urine

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