Rapid identification of bacteria directly from blood cultures by Co-magnetic bead enrichment and MALDI-TOF MS profiling

Rapid Pathogen Identification in Aqueous Humor Samples by Combining Fc-MBL@Fe 3 O 4 Enrichment and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Profiling

“…When samples of aqueous humor from patients with endophthalmitis are taken for examination, the gel-like aqueous humor and its constituents may interfere with the MS identification of bacteria ( 16 ). A previous study showed that Fc-MBL@Fe 3 O 4 could enrich clinically unknown bacteria from blood culture bottles for MALDI-TOF MS database identification ( 31 ). Here, we demonstrated that the enrichment efficiency of magnetic beads in aqueous humor was >(87.5 ± 5.0)%.…”

Algorithms push forward the application of MALDI–TOF mass fingerprinting in rapid precise diagnosis

“…Nowadays, along with the improvement of microbial enrichment techniques and database searching algorithms, accurate identification has been achieved for microbial mixtures and complex samples directly. Strategies like differential centrifugation, 61 affinity isolation, 62 magnetic precipitation, 63 and microchip separation 64 have been used to enrich and isolate microbes from patient or environmental specimens, either directly or after a rough culture. The isolated products, containing either single or multiple microbial species, are then directly subjected to mass fingerprinting for species identification.…”

Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria

“…More importantly, BC is not only time-consuming (e.g., incubation time: 5~7 days [3,[6][7][8]) but it is also difficult to detect the pathogenic bacteria with a slow-growing nature [4,7,9]. Furthermore, the BC-based method normally requires the associated techniques for the following bacteria purification (e.g., solid medium-based subculture, immunomagnetic microbead-based capture or microfluidic chip [3,4,6,10]) and for the final assays (e.g., PCR-, FISH-, MALDI-TOF MS-, Gram's stain-based assays [2][3][4]7,10]) to identify pathogenic bacteria or to test the antibiotic susceptibility [2]. These technical hurdles make it difficult to provide septic patients with antibiotic treatment efficiently and precisely, particularly within 6 h of the onset of persistent hypotension induced by septic shock, which is reported to greatly improve the survival rate of patients [9,11].…”