Rapid high-resolution immune electron microscopy of plant viruses

Milne, Robert G.; Luisoni, E.

doi:10.1016/0042-6822(75)90169-5

Cited by 123 publications

(74 citation statements)

References 7 publications

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“…Rapid and accurate diagnosis is often possible directly on crude preparations using precipitin tests, immunosorbent assays (Voller et al, 1976(Voller et al, , 1979 and various versions of immunoelectron microscopy (Milne & Luisoni, 1977). This is highly cost-effective and renders many of the other diagnostic tests redundant, providing of course that a clear positive answer can be obtained.…”

Section: Reactions With Likely Antiseramentioning

confidence: 99%

“…Pretreatment of grids with protein A of Staphylococcus aureus may enhance virus adsorption (Shukla & Gough, 1979) but meaningful results occur only under certain conditions (Lesemann & Paul, 1980;Milne, 1980). It may be useful to follow trapping of virus particles on serum-coated grids with decoration (Milne & Luisoni, 1977), particularly as trapping may be susceptible to bias due to non-specific adsorption of virus particles to the grids . Decoration of particles by antisera to distantly related viruses may be difficult to distinguish from non-specific decoration, however, especially in crude sap.…”

Section: Serology (A) Precipitin Tests Antigenic Analysis Of Isometrmentioning

confidence: 99%

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Guidelines for the Identification and Characterization of Plant Viruses

Hamilton

Edwardson

Francki

et al. 1981

Journal of General Virology

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Section: Reactions With Likely Antiseramentioning

confidence: 99%

Section: Serology (A) Precipitin Tests Antigenic Analysis Of Isometrmentioning

confidence: 99%

Guidelines for the Identification and Characterization of Plant Viruses

Hamilton

Edwardson

Francki

et al. 1981

Journal of General Virology

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“…Unambiguous identification of ZYMV particles in infected leaves can be achieved by immunosorbent electron microscopy (ISEM): virus particles are first trapped on a grid activated by antisera (Derrick, 1973) and subsequently decorated specifically by a homologous antiserum. Virus particles appear coated by a 'halo' of antibody molecules (Milne & Luisoni, 1977). ISEM procedures provide a high sensitivity and allow detection of viruses present in mixed infections, even at very low concentrations, as well as the establishment of serological relationships between strains or viruses (Lesemann et al, 1983;Wong et al, 1994).…”

Section: Electron Microscopymentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

221

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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“…The ISEM procedure was essentially the same as described by Milne & Luisoni (1977). Antisera were diluted to 1:20 in 67raM-sodium phosphate buffer pH 7.0, both in the coating and in the decoration steps.…”

Section: Immunospecific Electron Microscopy (Isem)mentioning

confidence: 99%

Characterization of Potyviruses from Tulip and Lily which Cause Flower-Breaking

Dekker

Derks²,

Asjes³

et al. 1993

Journal of General Virology

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Five viruses causing colour-breaking of tulip flowers were isolated from tulips and lilies. Tulip-breaking virus (TBV), tulip top-breaking virus (TTBV), tulip bandbreaking virus, Rembrandt tulip-breaking virus and lily mottle virus were all characterized as potyviruses by serology and potyvirus-specific PCR. Sequence analysis of amplified DNA fragments spanning a conserved area of the coat protein cistron ofpotyviruses was performed in order to classify the isolates as distinct viruses or strains. It appears that all tulip-breaking viruses are distinct viruses and TTBV was found to be strain-related to turnip mosaic virus.

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Rapid high-resolution immune electron microscopy of plant viruses

Cited by 123 publications

References 7 publications

Guidelines for the Identification and Characterization of Plant Viruses

Guidelines for the Identification and Characterization of Plant Viruses

Zucchini yellow mosaic virus

Characterization of Potyviruses from Tulip and Lily which Cause Flower-Breaking

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