Rapid headspace solid-phase microextraction-gas chromatographic–time-of-flight mass spectrometric method for qualitative profiling of ice wine volatile fraction

Šetková, Lucie; Risticevic, Sanja; Pawliszyn, Janusz

doi:10.1016/j.chroma.2007.02.052

Cited by 109 publications

(83 citation statements)

References 73 publications

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“…Compounds were loaded via the SPME fiber onto the GC column at 240 uC splitless injection for 30 s with a 0.75 mm ID injection port liner, and were separated on a DB-WAX column (30 m 6 0.25 mm ID 6 0.50 mm film thickness; Phenomenex). An internal standard, 2-heptanone, was loaded onto the pre-conditioned SPME fiber (2 min, 250 uC) for 30 s at 30 uC before sample exposure [20 ml vial with 4 g pump oil and 10 ml 2-heptanone (1 mg methanol ml 21 )] (Setkova et al, 2007). Samples were exposed to the fiber for 35 min at 30 uC.…”

Section: Methodsmentioning

confidence: 99%

Volatile organic compound production by organisms in the genus Ascocoryne and a re-evaluation of myco-diesel production by NRRL 50072

et al. 2010

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The Patagonian fungal endophyte NRRL 50072 is reported to produce a variety of mediumchain and highly branched volatile organic compounds (VOCs) that have been highlighted for their potential as fuel alternatives and are collectively termed myco-diesel. To assess the novelty of this observation, we determined the extent to which ten closely related Ascocoryne strains from commercial culture collections possess similar VOC production capability. DNA sequencing established a high genetic similarity between NRRL 50072 and each Ascocoryne isolate, consistent with its reassignment as Ascocoryne sarcoides. The Ascocoryne strains did not produce highly branched medium-chain-length alkanes, and efforts to reproduce the branched alkane production of NRRL 50072 were unsuccessful. However, we confirmed the production of 30 other products and expanded the list of VOCs for NRRL 50072 and members of the genus Ascocoryne. VOCs detected from the cultures consisted of short-and mediumchain alkenes, ketones, esters and alcohols and several sesquiterpenes. Ascocoryne strains NRRL 50072 and CBS 309.71 produced a more diverse range of volatiles than the other isolates tested. CBS 309.71 also showed enhanced production compared with other strains when grown on cellulose agar. Collectively, the members of the genus Ascocoryne demonstrated production of over 100 individual compounds, with a third of the short-and medium-chain compounds also produced when cultures were grown on a cellulose substrate. This comparative production analysis could facilitate future studies to identify and manipulate the biosynthetic machinery responsible for production of individual VOCs, including several that have a potential application as biofuels.

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Section: Methodsmentioning

confidence: 99%

Volatile organic compound production by organisms in the genus Ascocoryne and a re-evaluation of myco-diesel production by NRRL 50072

et al. 2010

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“…The works of Camara, Alves and Marques (2007) should be cited, which distinguished Madeira wines made from Boal, Malvazia, Sercial, and Verdelho working on the free fraction of monoterpenols and norisoprenoids; and in France, Pereira et al (2007), working with metabolic profile based on proton nuclear magnetic resonance spectra, differentiated Cabernet Franc, Cabernet Sauvignon, and Merlot wines; Setkova, Risticevic and Pawliszyn (2007), using chromatographic-time-of-flight mass spectrometric method, differentiated ice wines from Canada and the Czech Republic. In addition to varietal wines, it is possible to discriminate wines made from clones from only one grape variety.…”

Section: Varietal Winementioning

confidence: 99%

Discrimination of Brazilian red wines according to the viticultural region, varietal, and winery origin

Miele¹,

Rizzon²,

Zanus³

2010

Ciênc. Tecnol. Aliment.

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OriginalRecebido para publicação em 15/9/2008 Aceito para publicação em 18/7/2009 (003750) 1 Embrapa Uva e Vinho, CP 130, Brasil, rizzon@cnpuv.embrapa.br; zanus@cnpuv.embrapa IntroductionThe annual Brazilian grape production is over one million tons, half sold as table grapes and the other half as grapes for processing. Considering the latter segment, wine is an important product due to the added value it transfers to wineries and grape growers.Nevertheless, the Brazilian wine industry has low competitiveness. Today, most of the fine wine consumed in Brazil is imported from Chile, Argentina, and European countries. To mitigate this effect, it is necessary to improve wine competitiveness, which could be reached with tipicity, quality, and price. Thus, some actions are being taken by wine producers and wine sellers.There are few studies in Brazil regarding viticultural region, varietal wines, and wineries. In fact, the characterization of Cabernet Franc wines from five different areas of the Serra Gaúcha should be pointed out (MIELE et al., 1996;MIELE, 1999). In another study, discrimination between Brazilian, Argentinean, and Uruguayan wines was established (RIZZON; MIELE; ROSIER, 1997). However, there are many works carried out concerning physicochemical and/or sensory characterization of varietal wines (BEHRENS; SILVA, 2000;MIELE, 2000MIELE, , 2001MIELE, , 2002MIELE, , 2004MANFROI et al., 2006;MENEGUZZO et al., 2006;MIELE; Resumo O trabalho avaliou a composição físico-química de 171 vinhos tintos brasileiros elaborados na safra de 2006, representados por 21 varietais. Os vinhos foram elaborados por 58 vinícolas localizadas em sete regiões vitícolas do País com latitudes variando de 9º a 31º Sul. A análise físico-química foi feita em 2006 e a discriminação entre as regiões vitícolas, os vinhos varietais e as vinícolas foi realizada através da análise de componentes principais (ACP). Os principais resultados mostram que ao se considerar as regiões vitícolas, os vinhos elaborados em São Joaquim caracterizaram-se por valores mais elevados de A420, A520, A620, intensidade de cor, compostos fenólicos totais, antocianinas e extrato seco, enquanto que os de Toledo apresentaram valores mais baixos dessas variáveis; os do Vale do São Francisco tiveram valores mais elevados de potássio, pH, densidade e acidez volátil; os da Serra do Nordeste A, maior acidez titulável; e os do Planalto Superior B, matiz mais elevado. No que se relaciona aos vinhos varietais, a ACP discriminou os vinhos feitos com as variedades Ancellotta, Teroldego, Egiodola, Refosco, Marselan, Cabernet Sauvignon, Pinotage, Pinot Noir, Malbec, Arinarnoa, Barbera e Alfrocheiro. Com relação às vinícolas, vinte e duas delas foram discriminadas por apresentarem parâmetros mais elevados de determinadas variáveis, i.e., três delas por terem maior intensidade de cor; três, por seu matiz; oito, pelo teor de álcool; seis, por potássio, extrato seco, densidade e pH; e duas, pela acidez titulável. Palavras-chave: composição físico-química; origem geográf...

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“…Since its development, this technique has become very popular and gained growing acceptance and increasing use in routine laboratories applications mainly to the sampling and analysis of environmental [33], food [34][35][36], forensic [37] and pharmaceutical samples [38,39]. Typically followed by GC in combination with mass spectrometric detection (MS) [40][41][42] or its multidimensional alternative, comprehensive two-dimensional GC (GC × GC-ToFMS) [43][44][45], SPME technique has been successfully used for wine samples.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of high-throughput miniaturized sorbent- and solid phase microextraction techniques combined with gas chromatography–mass spectrometry analysis for a rapid screening of volatile and semi-volatile composition of wines—A comparative study

Mendes¹,

Gonçalves²,

Câmara³

2012

Talanta

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a b s t r a c tIn this study the feasibility of different extraction procedures was evaluated in order to test their potential for the extraction of the volatile (VOCs) and semi-volatile constituents (SVOCs) from wines. In this sense, and before they could be analysed by gas chromatography-quadrupole first stage masss spectrometry (GC-qMS), three different high-throughput miniaturized (ad)sorptive extraction techniques, based on solid phase extraction (SPE), microextraction by packed sorbents (MEPS) and solid phase microextraction (SPME), were studied for the first time together, for the extraction step. To achieve the most complete volatile and semi-volatile signature, distinct SPE (LiChrolut EN, Poropak Q, Styrene-Divinylbenzene and Amberlite XAD-2) and MEPS (C 2 , C 8 , C 18 , Silica and M1 (mixed C 8 -SCX)) sorbent materials, and different SPME fibre coatings (PA, PDMS, PEG, DVB/CAR/PDMS, PDMS/DVB, and CAR/PDMS), were tested and compared. All the extraction techniques were followed by GC-qMS analysis, which allowed the identification of up to 103 VOCs and SVOCs, distributed by distinct chemical families: higher alcohols, esters, fatty acids, carbonyl compounds and furan compounds. Mass spectra, standard compounds and retention index were used for identification purposes.SPE technique, using LiChrolut EN as sorbent (SPE LiChrolut EN ), was the most efficient method allowing for the identification of 78 VOCs and SVOCs, 63 and 19 more than MEPS and SPME techniques, respectively. In MEPS technique the best results in terms of number of extractable/identified compounds and total peak areas of volatile and semi-volatile fraction, were obtained by using C 8 resin whereas DVB/CAR/PDMS was revealed the most efficient SPME coating to extract VOCs and SVOCs from Bual wine. Diethyl malate (18.8 ± 3.2%) was the main component found in wine SPE LiChrolut EN extracts followed by ethyl succinate (13.5 ± 5.3%), 3-methyl-1-butanol (13.2 ± 1.7%), and 2-phenylethanol (11.2 ± 9.9%), while in SPME DVB/CAR/PDMS technique 3-methyl-1-butanol (43.3 ± 0.6%) followed by diethyl succinate (18.9 ± 1.6%), and 2-furfural (10.4 ± 0.4%), are the major compounds. The major VOCs and SVOCs isolated by MEPS C8 were 3-methyl-1-butanol (26.8 ± 0.6%, from wine total volatile fraction), diethyl succinate (24.9 ± 0.8%), and diethyl malate (16.3 ± 0.9%). Regardless of the extraction technique, the highest extraction efficiency corresponds to esters and higher alcohols and the lowest to fatty acids.Despite some drawbacks associated with the SPE procedure such as the use of organic solvents, the time-consuming and tedious sampling procedure, it was observed that SPE LiChrolut EN , revealed to be the most effective technique allowing the extraction of a higher number of compounds (78) rather than the other extraction techniques studied.

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Rapid headspace solid-phase microextraction-gas chromatographic–time-of-flight mass spectrometric method for qualitative profiling of ice wine volatile fraction

Cited by 109 publications

References 73 publications

Volatile organic compound production by organisms in the genus Ascocoryne and a re-evaluation of myco-diesel production by NRRL 50072

Volatile organic compound production by organisms in the genus Ascocoryne and a re-evaluation of myco-diesel production by NRRL 50072

Discrimination of Brazilian red wines according to the viticultural region, varietal, and winery origin

Effectiveness of high-throughput miniaturized sorbent- and solid phase microextraction techniques combined with gas chromatography–mass spectrometry analysis for a rapid screening of volatile and semi-volatile composition of wines—A comparative study

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