Rapid flow-through enzyme immunoassay of progesterone in whole cows' milk

Samsonova, J.V.; Safronova, V.A.; Осипов, А. И.

doi:10.1016/j.ab.2018.01.011

Cited by 20 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flow-through assays can be prepared in different ways. Passive flow-through assays consist of LFIA materials, but in a stacked arrangement, with the membrane biofunctionalized with capture antibodies on top, and the conjugate and absorbent pads layered underneath or as flow-through enzyme-linked immunosorbent assays (ELISAs) [37][38][39]. An alternative flow-through approach is to insert a biofunctionalized membrane into a syringe filter holder, applying manual or mechanical pressure to the syringe to actively control the vertical flow of the reagents and the sample [40,41].…”

Section: Introductionmentioning

confidence: 99%

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

2019

View full text Add to dashboard Cite

(1) Background: The lack of globally standardized allergen labeling legislation necessitates consumer-focused multiplexed testing devices. These should be easy to operate, fast, sensitive and robust. (2) Methods: Herein, we describe the development of three different formats for multiplexed food allergen detection, namely active and passive flow-through assays, and lateral flow immunoassays with different test line configurations. (3) Results: The fastest assay time was 1 min, whereas even the slowest assay was within 10 min. With the passive flow approach, the limits of detection (LOD) of 0.1 and 0.5 ppm for total hazelnut protein (THP) and total peanut protein (TPP) in spiked buffer were reached, or 1 and 5 ppm of THP and TPP spiked into matrix. In comparison, the active flow approach reached LODs of 0.05 ppm for both analytes in buffer and 0.5 and 1 ppm of THP and TPP spiked into matrix. The optimized LFIA configuration reached LODs of 0.1 and 0.5 ppm of THP and TPP spiked into buffer or 0.5 ppm for both analytes spiked into matrix. The optimized LFIA was validated by testing in 20 different blank and spiked matrices. Using device-independent color space for smartphone analysis, two different smartphone models were used for the analysis of optimized assays.

show abstract

Section: Introductionmentioning

confidence: 99%

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

2019

View full text Add to dashboard Cite

show abstract

“…The most widely known protein‐dependent approaches used for the detection of allergens are based on antigen–antibody interactions and are accessible in various formats such as ELISA (Zeng et al., 2019; Orcajo, Lavilla, & Martínez‐De‐Marañón, 2019), LFDs (Chen et al., 2017), dipstick tests (Samsonova, Safronova, & Osipov, 2018), and immunoblotting. At present, special attention has been given to pioneering technologies for the analysis of allergens, specifically immunosensors and MS platforms (Costa et al., 2017).…”

Section: Analytical Methods For the Detection Of Wp Allergensmentioning

confidence: 99%

Whey allergens: Influence of nonthermal processing treatments and their detection methods

Khan

Lin

Ahmed

et al. 2021

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Whey and its components are recognized as value-added ingredients in infant formulas, beverages, sports nutritious foods, and other food products. Whey offers opportunities for the food industrial sector to develop functional foods with potential health benefits due to its unique physiological and functional attributes. Despite all the above importance, the consumption of whey protein (WP) can trigger hypersensitive reactions and is a constant threat for sensitive individuals. Although avoiding such food products is the most successful approach, there is still a chance of incorrect labeling and cross-contamination during food processing. As whey allergens in food products are cross-reactive, the phenomenon of homologous milk proteins of various species may escalate to a more serious problem. In this review, nonthermal processing technologies used to prevent and eliminate WP allergies are presented and discussed in detail.These processing technologies can either enhance or mitigate the impact of potential allergenicity. Therefore, the development of highly precise analytical technologies to detect and quantify the existence of whey allergens is of considerable importance. The present review is an attempt to cover all the updated approaches used for the detection of whey allergens in processed food products.Immunological and DNA-based assays are generally used for detecting allergenic proteins in processed food products. In addition, mass spectrometry is also employed as a preliminary technique for detection. We also highlighted the latest improvements in allergen detection toward biosensing strategies particularly immunosensors and aptasensors.

show abstract

“…These are still most commonly exploited and available in different formats. These are based on antigen-antibody interactions including enzyme-linked immunosorbent assay (ELISA) [37,38], dipstick tests [39], and lateral flow devices (LFD) [40]. Furthermore, polymerase chain reaction (PCR) [41,42] and liquid chromatography-mass spectrometry (LC-MS) [26] platforms are mostly explored alternatives of antibody-based assays.…”

Section: Analytical Standard Methods For the Milk Allergens Detectionmentioning

confidence: 99%

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Nehra

Lettieri

Dilbaghi

et al. 2019

Sensors

View full text Add to dashboard Cite

Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins’ presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

show abstract

Rapid flow-through enzyme immunoassay of progesterone in whole cows' milk

Cited by 20 publications

References 19 publications

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection

Whey allergens: Influence of nonthermal processing treatments and their detection methods

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Contact Info

Product

Resources

About