Rapid Electrochemical Detection of Coronavirus SARS-CoV-2.

Chaibun, Thanyarat; Puenpa, Jiratchaya; Ngamdee, Tatchanun; Boonapatcharoen, Nimaradee; Athamanolap, Pornpat; O’Mullane, Anthony P.; Vongpunsawad, Sompong; Poovorawan, Yong; Yin, Lee Su; Lertanantawong, Benchaporn

doi:10.21203/rs.3.rs-86933/v1

Cited by 8 publications

(12 citation statements)

References 30 publications

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“…This isothermal amplification method has been applied to detection of numerous viruses, including MERS-CoV. 151 It is noteworthy that other isothermal amplification techniques (some of which will be discussed below) are also in existence and have been used for viral detection, for instance: recombinase polymerase amplification (RPA); 152 rolling cycle amplification (RCA); 153 and nucleic acid sequence-based amplification (NASBA). 154 We will discuss LAMP as an exemplar.…”

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Science’s Response to COVID-19

Aye¹,

Long²

2021

Preprint

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CoVID-19 is a multi-symptomatic disease which has made a global impact due to its ability to spread rapidly, and its relatively high mortality rate. Beyond the heroic efforts to develop vaccines, which we will not discuss, the response of scientists and clinicians to this complex problem has reflected the need to detect CoVID-19 rapidly, to diagnose patients likely to show adverse symptoms, and to treat severe and critical CoVID-19. Here we aim to encapsulate these varied and sometimes conflicting approaches and the resulting data in terms of chemistry and biology. In the process we highlight emerging concepts, and potential future applications that may arise out of this immense effort.

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Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Science’s Response to COVID-19

Aye¹,

Long²

2021

Preprint

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“…An electrochemical biosensor based on RNA amplification using isothermal RCA and differential pulse voltammetry (DPV) was reported (Fig. 7 ) [ 227 ]. It involves 4 simple steps: (1) sample collection, (2) extraction of RNA/DNA, (3) hybridization using RCA reaction, and (4) detection (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…It involves 4 simple steps: (1) sample collection, (2) extraction of RNA/DNA, (3) hybridization using RCA reaction, and (4) detection (Fig. 7 ) [ 227 ]. The extracted RNA/DNA was purified and separated using magnetic beads coated with streptavidin.…”

Section: Introductionmentioning

confidence: 99%

“…This assay offers a LOD of 1 copy/μL of N and S genes (Fig. 7 ) [ 227 ]. This method is a one-step sandwich hybridization assay, rapid (2 h), sensitive, and accurate (100% concordance result with qRT-PCR).…”

Section: Introductionmentioning

confidence: 99%

“…This method is a one-step sandwich hybridization assay, rapid (2 h), sensitive, and accurate (100% concordance result with qRT-PCR). Furthermore, the potentiostat (PalmSen4) is portable, offering on-site, real-time diagnostic tests for COVID-19 [ 227 ].

Fig.…”

Section: Introductionmentioning

confidence: 99%

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Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

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Rapid and High‐Throughput SARS‐CoV‐2 RNA Detection without RNA Extraction and Amplification by Using a Microfluidic Biochip

Chu

Qiu

Wang

et al. 2022

Chemistry A European J

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The ongoing outbreak of the severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2) has spread globally and poses a threat to public health and National economic development. Rapid and high‐throughput SARS‐CoV‐2 RNA detection without the need of RNA extraction and amplification remain a key challenge. In this study, a new SARS‐CoV‐2 RNA detection strategy using a microfluidic biochip for the rapid and ultrasensitive detection of SARS‐CoV‐2 without RNA extraction and amplification was developed. This new strategy takes advantage of the specific SARS‐CoV‐2 RNA and probe DNA reaction in the microfluidic channel, fluorescence signal regulation by nanomaterials, and accurate sample control by the microfluidic chip. It presents an ultralow limit of detection of 600 copies mL−1 in a large linear detection regime from 1 aM to 100 fM. Fifteen samples were simultaneously detected in 40 min without the need for RNA purification and amplification. The detection accuracy of the strategy was validated through quantitative reverse transcription polymerase chain reaction (qRT‐PCR), with a recovery of 99–113 %. Therefore, the SARS‐CoV‐2 RNA detection strategy proposed in this study can potentially be used for the quantitative diagnosis of viral infectious diseases.

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Rapid Electrochemical Detection of Coronavirus SARS-CoV-2.

Cited by 8 publications

References 30 publications

Science’s Response to COVID-19

Science’s Response to COVID-19

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Rapid and High‐Throughput SARS‐CoV‐2 RNA Detection without RNA Extraction and Amplification by Using a Microfluidic Biochip

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