Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification

Kim, Jong-Gil; Baek, Seung Hoon; Kim, Seungrok; Kim, Hae In; Lee, Seung Woo; Phan, Le Minh Tu; Kailasa, Suresh Kumar; Park, Tae Jung

doi:10.1016/j.talanta.2018.08.019

Cited by 32 publications

(21 citation statements)

References 29 publications

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“…In contrast, lower concentrations of Mg 2+ fail to amplify the target genes. In this study, the optimal concentration of Mg 2+ was 6.0 mM for the sssvLAMP method, which was consistent with previous studies on the detection of ctxA gene of V. cholerae (Engku Nur Syafirah et al, 2018), dengue viruses (Kim et al, 2018), and Mycoplasma hyopneumoniae (Liu et al, 2015), but less than those (8 mM) for the detection of ctxA and ompW genes of V. cholerae (Okada et al, 2010;Srisuk et al, 2010).…”

Section: Discussionsupporting

confidence: 91%

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

Chen

et al. 2019

Front. Microbiol.

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Section: Discussionsupporting

confidence: 91%

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

Chen

et al. 2019

Front. Microbiol.

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“…Considering these circumstances, several groups have developed new biosensor system for dynamic NS1 quantification [1, 11, 18, 19]. Immunosensors using fluorescent nanoparticles [19–23], surface plasmon resonance (SPR) methods [24], DNA-based bioassays [3, 18, 25–27], and electrochemical detection methods [5, 12, 28–30] have been reported recently. For example, electrochemical detection is widely used in the developing of label-free and portable point-of-care biosensors [5, 28, 29].…”

Section: Introductionmentioning

confidence: 99%

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

et al. 2019

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Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1–BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing.

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“…(1) For human: severe acute respiratory syndrome coronavirus (SARS-CoV) [58,59], dengue viruses [60,61], hepatitis A viruses [12], hepatitis B viruses [7,8], hepatitis C viruses [62], human severe fever of bunya viruses with thrombocytopenia syndrome (SFTS) [63][64][65], influenza A H1N1 viruses infecting human [34], Zika [48], Enterovirus 71 for human hand, foot, and mouth disease (HFMD) [66], human immun-odeficiency virus 1 (HIV-1) [30], Zaire ebolaviruses [67], rotaviruses in fecal samples for acute viral gastroenteritis [68]. (2) For arthropod: Chikungunya viruses [11] and arthropodborne West Nile viruses [69].…”

Section: Applications Of Lampmentioning

confidence: 99%

Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

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a b s t r a c tColorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

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Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification

Cited by 32 publications

References 29 publications

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

Simple Visualized Detection Method of Virulence-Associated Genes of Vibrio cholerae by Loop-Mediated Isothermal Amplification

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

Colorimetric biosensors for point-of-care virus detections

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