Rapid disappearance from serum of intravenously injected rat myeloma IgA and its secretion into bile

Jackson, G. D. F.; Lemaître‐Coelho, I.; Vaerman, Jean‐Pierre; Bazin, Hervé; Beckers, Andrée

doi:10.1002/eji.1830080210

Cited by 193 publications

(51 citation statements)

References 22 publications

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“…We recently showed a selective plasma-to-bile transport of p-IgA in humans (29). However, we and others (29,42) have shown that this transport is quantitatively much less important in humans than in rats and rabbits in which a major SC-dependent transhepatocyte transport of p-IgA occurs (43)(44)(45). Our present lack of serum p-IgA increase in human B Obst, unlike in rats and rabbits in which B Obst results in a 10 times (or larger) increase in serum p-IgA (45,46), is thus another argument for major species differences in hepatic transport of p-IgA into bile.…”

Section: Resultsmentioning

confidence: 79%

Changes in size, subclass, and metabolic properties of serum immunoglobulin A in liver diseases and in other diseases with high serum immunoglobulin A.

et al. 1983

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A B ST R A CT We have studied the relative contributions of monomeric (m-) and polymeric IgA (p-IgA) and of IgAl and IgA2 to total serum IgA in healthy adults and patients with liver disease (LD) or with other diseases and high serum IgA. Serum concentration of total secretory component (SC) was also determined. In addition, fractional catabolic rates (FCR) and synthetic rates for both m-and p-IgA were measured in nine controls and nine cirrhotics. Our results support four main conclusions: (a) In healthy adults, intravascular p-IgA contributes to only 4-22% (mean 12%) of serum IgA, because its FCR and synthetic rate are approximately two times higher and four times smaller, respectively, than those of intravascular mIgA. (b) In LD, biliary obstruction does not result in a significant increase in serum p-IgA unlike in rats and rabbits, indicating that in humans the SC-dependent biliary transport of p-IgA plays a much less significant role in selective removal of p-IgA from plasma than in rats and rabbits. (c) In contrast to biliary obstruction, parenchymal LD results in a significant and preferential increase in serum p-IgA, which in cirrhotics correlates with a selective reduction of the p-IgA-FCR. This supports a role for the human liver in selective removal of p-IgA from plasma, but another mechanism than the SC-dependent biliary transport should be considered. (d) Total SC, p-IgA, and IgA2 in serum are unlinked parameters, not necessarily reflecting mucosal events. A marked increase in serum SC occurs Address reprint requests to Dr. Delacroix, ICP-MEXP, Brussels, Belgium.

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Section: Resultsmentioning

confidence: 79%

Changes in size, subclass, and metabolic properties of serum immunoglobulin A in liver diseases and in other diseases with high serum immunoglobulin A.

et al. 1983

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“…At several mucosal sites secretory component (SC)', an epithelial cell surface glycoprotein, acts as a specific receptor and initiates an endocytotic vesicular transport to lumen of polymeric IgA (p-IgA) and IgM synthesized by submucosal plasmacytes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). Recently, it has been shown that pIgA is also actively transported from serum into bile by the hepatocyte of the rat and other mammals (11)(12)(13)(14)(15). Suggested roles for this transport are reinforcement of intestinal immunity (16,17) and clearance of p-IgA antibodies and immune complexes from blood (18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

Selective transport of polymeric immunoglobulin A in bile. Quantitative relationships of monomeric and polymeric immunoglobulin A, immunoglobulin M, and other proteins in serum, bile, and saliva.

Delacroix¹,

Hodgson²,

McPherson³

et al. 1982

J. Clin. Invest.

210

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A B S T R A C T In 17 adults, serum, hepatic bile, and saliva samples were analyzed for their sedimentation profile of IgA and secretory component (SC), and for their concentrations of albumin, orosomucoid, transferrin, IgG, IgA, a2-macroglobulin (a2M), IgM, and SC. Polymeric IgA(p-IgA) averaged 13% (50-700 ltg/ ml) of total IgA in serum, 70% (43-88%) in bile, and 93% (74-98%) in saliva. Most of the p-IgA in bile sedimented with SC, which also occurred free (8-44%), and with IgM. In bile, albumin (155-1,485 ug/ml) was the predominant protein, followed by IgG (32-480 gg/ml), and total IgA (37-209 ,g/ml). In saliva, p-IgA (72-902 Mig/ml) predominated, followed by albumin (16-385 ug/ml) and IgG (9-178 Mg/ml). Secretion-toserum albumin-relative concentration ratios (S/S-ARCR = 1 for albumin) in bile averaged 22 for p-IgA, 1.91 for IgM, 1.28 for monomeric IgA (m-IgA), 0.70 for IgG, and 0.57 for a2M, indicating for p-IgA, IgM, and to a lesser extent for m-IgA, a selective excretion into bile. In saliva, a 16-fold greater selective excretion of p-IgA (mean S/S-ARCR = 354) was found. Labeled m-and p-IgA were injected intravenously into five patients. Specific activities indicated that for p-IgA 50% was serum derived in bile, as compared with 2% in saliva, and to 85% for m-IgA in bile. In the patient with the highest excretion of 125I-p-IgA in bile, only 2.8% of the injected dose was recovered in bile within 24 h after injection. Compared with rats and rabbits, Dr. Delacroix is an Aspirant for the Fond National de la Recherche Scientifique, Brussels, Belgium. His present address is UCL-ICP-MEXP,

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“…It has only recently been possible (Fisher et al, 1979) to reveal 0.12% of SIgM (in comparison with SIgA) but neither IgG2a nor IgE nor monomeric IgA. Orlans et al (1978) and Jackson et al (1978) show through different methods that the monomeric IgA of the sera which actively enter the bile, thus become polymeric SIgA provided which a secretory component (S C). On the contrary when rat's choledoch has been ligatured, SIgA and S C can be found free in the serum (LemaitreCoehlo, Jackson and Vaerman, 1978).…”

Section: Discussionmentioning

confidence: 99%

“…Orlans et al (1978) and Jackson et al (1978) show through different methods that the monomeric IgA of the sera which actively enter the bile, thus become polymeric SIgA provided which a secretory component (S C). On the contrary when rat's choledoch has been ligatured, SIgA and S C can be found free in the serum (LemaitreCoehlo, Jackson and Vaerman, 1978). Furthermore it has been proved (Fisher et al, 1979) that the SIgA are secreted through the association of polymeric Ig A and the S C in the liver.…”

Section: Discussionmentioning

confidence: 99%

Trichinella spiralis in rats : in vivo effects of the bile and in vitro action of secretory IgA from bile

Jacqueline

Crinquette²,

Bout

et al. 1981

Ann. Parasitol. Hum. Comp.

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SUM M ARY.The biliary secretion of rats is diverted from the choledoch duct to the bladder. These rats are resuscitated by sub-cutaneous injections of a salt solution and then infected with Trichinella spiralis larvae. It is shown that the number of adult worms is increased (+ 66 %); as is the female larvae production (+ 51%); the females' lenght is also increased (+ 25%). 40 days after the infection the number of muscular larvae is considerably increased (+ 79%) in comparison with control rats. Secretory IgA (SIgA) from rats bile were tested in vitro on the female production of larvae. This larvae production was more inhibited (59%) by immune SIgA than by control SIgA (25%).Trichinella spiralis chez le rat : effets in vivo de la bile et action in vitro des IGA secrétoires de la bile.La sécrétion biliaire des rats est dérivée du canal cholédoque dans la vessie. Ces rats sont « réanimés » par des injections sous cutanées d'une solution saline et sont infestés par des larves de Trichinella spiralis. Il est montré que le nombre de vers adultes est augmenté (+ 66%) de même que la production larvaire des femelles (+ 51 %) ; la taille des femelles étant également accrue (+ 25 %). 40 jours après l'infestation le nombre de larves musculaires est considérablement augmenté (+ 79 %) par rapport aux rats témoins. L'action des IgA secrétoires (SIgA), séparées de la bile des rats et testée in vitro sur la production de larves, est inhibée (59 %) de façon plus importante par les SIgA immunes que par les SIgA non spécifiques (25 %).

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Rapid disappearance from serum of intravenously injected rat myeloma IgA and its secretion into bile

Cited by 193 publications

References 22 publications

Changes in size, subclass, and metabolic properties of serum immunoglobulin A in liver diseases and in other diseases with high serum immunoglobulin A.

Changes in size, subclass, and metabolic properties of serum immunoglobulin A in liver diseases and in other diseases with high serum immunoglobulin A.

Selective transport of polymeric immunoglobulin A in bile. Quantitative relationships of monomeric and polymeric immunoglobulin A, immunoglobulin M, and other proteins in serum, bile, and saliva.

Trichinella spiralis in rats : in vivo effects of the bile and in vitro action of secretory IgA from bile

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