Trichinella spiralis in rats : in vivo effects of the bile and in vitro action of secretory IgA from bile

Abstract

SUM M ARY.The biliary secretion of rats is diverted from the choledoch duct to the bladder. These rats are resuscitated by sub-cutaneous injections of a salt solution and then infected with Trichinella spiralis larvae. It is shown that the number of adult worms is increased (+ 66 %); as is the female larvae production (+ 51%); the females' lenght is also increased (+ 25%). 40 days after the infection the number of muscular larvae is considerably increased (+ 79%) in comparison with control rats. Secretory IgA (SIgA) from rats bile were tested in vitro on the female production of larvae. This larvae production was more inhibited (59%) by immune SIgA than by control SIgA (25%).Trichinella spiralis chez le rat : effets in vivo de la bile et action in vitro des IGA secrétoires de la bile.La sécrétion biliaire des rats est dérivée du canal cholédoque dans la vessie. Ces rats sont « réanimés » par des injections sous cutanées d'une solution saline et sont infestés par des larves de Trichinella spiralis. Il est montré que le nombre de vers adultes est augmenté (+ 66%) de même que la production larvaire des femelles (+ 51 %) ; la taille des femelles étant également accrue (+ 25 %). 40 jours après l'infestation le nombre de larves musculaires est considérablement augmenté (+ 79 %) par rapport aux rats témoins. L'action des IgA secrétoires (SIgA), séparées de la bile des rats et testée in vitro sur la production de larves, est inhibée (59 %) de façon plus importante par les SIgA immunes que par les SIgA non spécifiques (25 %).

Effect of fox, pig, sheep, and poultry bile on the establishment of domestic and sylvatic species of Trichinella in rats

“…is not known. Possible actions of bile on Trichinella larvae are, the digestion of larvae as it has been shown in the case of chicken bile (Barriga, 1981), the inhibition of larvae by immune secretory IgA from bile (Jacqueline et al 1981), the alteration of the parasite's behavioural and nutritional status by exposure to bile (Stewart et al 1987), the structural reorganization of the surface coat of larvae by exposure to bile (Smith, Selkirk & Gounaris, 2000), and the modification of tyrosine phosphorylated proteins on the larval cuticle by incubation of the larvae with bile (Allegretti et al 2001). Nevertheless, all the above mechanism explain the detrimental effect of bile on Trichinella larvae.…”

Genetic Background Affects the Mucosal Secretory IgA Levels, Parasite Burden, Lung Inflammation, and Mouse Susceptibility toAscaris suumInfection

“…For instance, swine IgA-positive cells have been associated with protection against ascariasis (13,14), and the size of the Peyer's patch and the levels of total intestinal IgA have been related to Trichinella infection (15), reduction in fertility was observed when mucous IgA was added to adult worm cultures (16), and in the rat, the depletion of antigen-specific IgA reduced eosinophil antibody-dependent cellular cytotoxicity (ADCC) and was associated with impaired larval development (17). Likewise, reinforcing the protective role of IgA, there are many articles showing S-IgA associated with resistance against bacteria, protozoa, and helminths (16,(18)(19)(20)(21)(22). In addition, an S-IgA-mediated inflammatory response that included induction of neutrophil respiratory burst (23) and eosinophil degranulation (24) has been described as one of several protective mechanisms against pathogens.…”