Rapid diagnostic PCR assays for members of the Culicoides obsoletus and Culicoides pulicaris species complexes, implicated vectors of bluetongue virus in Europe

Nolan, D.T.; Carpenter, Simon; Barber, James; Mellor, P. S.; Dallas, John F.; Mordue, A. J.; Piertney, Stuart B.

doi:10.1016/j.vetmic.2007.03.019

Cited by 143 publications

(179 citation statements)

References 40 publications

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“…Until recently four species within this subgenus have been identified within this region and are commonly referred to as the Obsoletus group, despite a lack of monophyly: Culicoides obsoletus (Meigen) and Culicoides scoticus Downes & Kettle; Culicoides dewulfi Goetghebuer and Culicoides chiopterus (Meigen) [24][25][26]. Several additional cryptic species have also recently been proposed whose taxonomic status, prevalence, abundance and involvement in transmission of arboviruses remains uncertain [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…Several additional cryptic species have also recently been proposed whose taxonomic status, prevalence, abundance and involvement in transmission of arboviruses remains uncertain [27][28][29]. To date, these cryptic species have not been identified in the UK, despite a study that examined 79 individuals of the subgenus Avaritia using a 472 bp region of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) across ten geographically disparate sampling points [25].…”

Section: Introductionmentioning

confidence: 99%

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Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom

Barber¹,

Harrup²,

Silk³

et al. 2018

Parasites Vectors

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Background: Culicoides biting midges (Diptera: Ceratopogonidae) are responsible for the biological transmission of internationally important arboviruses of livestock. In 2011, a novel Orthobunyavirus was discovered in northern Europe causing congenital malformations and abortions in ruminants. From field studies, Culicoides were implicated in the transmission of this virus which was subsequently named Schmallenberg virus (SBV), but to date no assessment of susceptibility to infection of field populations under standardised laboratory conditions has been carried out. We assessed the influence of membrane type (chick skin, collagen, Parafilm M®) when offered in conjunction with an artificial blood-feeding system (Hemotek, UK) on field-collected Culicoides blood-feeding rates. Susceptibility to infection with SBV following blood-feeding on an SBV-blood suspension provided via either (i) the Hemotek system or via (ii) a saturated cotton wool pledglet was then compared. Schmallenberg virus susceptibility was defined by RT-qPCR of RNA extractions of head homogenates and related to Culicoides species and haplotype identifications based on the DNA barcode region of the mitochondrial cytochrome c oxidase 1 (cox1) gene. Results: Culicoides blood-feeding rates were low across all membrane types tested (7.5% chick skin, 0.0% for collagen, 4.4% Parafilm M®, with 6029 female Culicoides being offered a blood meal in total). Susceptibility to infection with SBV through membrane blood-feeding (8 of 109 individuals tested) and pledglet blood-feeding (1 of 94 individuals tested) was demonstrated for the Obsoletus complex, with both C. obsoletus (Meigen) and C. scoticus Downes & Kettle susceptible to infection with SBV through oral feeding. Potential evidence of cryptic species within UK populations was found for the Obsoletus complex in phylogenetic analyses of cox1 DNA barcodes of 74 individuals assessed from a single field-site. Conclusions: Methods described in this study provide the means to blood-feed Palaearctic Culicoides for vector competence studies and colonisation attempts. Susceptibility to SBV infection was 7.3% for membrane-fed members of the subgenus Avaritia and 1.1% for pledglet-fed. Both C. obsoletus and C. scoticus were confirmed as being susceptible to infection with SBV, with potential evidence of cryptic species within UK Obsoletus complex specimens, however the implications of cryptic diversity in the Obsoletus complex on arbovirus transmission remains unknown.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom

Barber¹,

Harrup²,

Silk³

et al. 2018

Parasites Vectors

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“…A molecular-genetic method based on the development of species-specific primers offers the opportunity of simplifying the identification process of the Culicoides midges (Nolan et al 2007;Stephan et al 2009). Numerous studies (Dallas et al 2003;Li et al 2003;Cêtre-Sossah et al 2004;Wörheide et al 2004;Perrin et al 2006) dealing with a similar problem found the noncoding genetic region of the internal transcribed spacer 1 (ITS-1) of the mitochondrial ribosomal DNA and mitochondrial cytochrome c oxidase subunit 1 (COI) to be highly promising in order to establish a reliable polymerase chain reaction (PCR)-based method for species identification.…”

Section: Introductionmentioning

confidence: 99%

A survey of biting midges of the genus Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) in NE Bulgaria, with respect to transmission of avian haemosporidians

Bobeva

Zehtindjiev

Bensch

et al. 2013

Acta Parasitologica

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This study presents data from a molecular survey of the species of the genus Culicoides from the region of Kalimok Field Station (NE Bulgaria) and haemosporidian parasites occurring in them in order to investigate the host-parasite specificity of haemosporidians to their dipteran vectors. The identification of Culicoides spp. was carried out by morphological and molecular-genetic methods. We collected and analysed 230 individuals of the genus Culicoides. Nine species were found. Eight species were identified morphologically; Culicoides obsoletus, C. riethi, C. newsteadi, C. circumscriptus, C. festivipennis, C. punctatus, C. pictipennis and C. puncticollis. The ninth species might be classified as either of C. nubeculosus or C. riethi and its identification needs additional investigations. The total prevalence of Haemoproteus in the examined biting midges was 2.17%. Three individuals of C. pictipennis were infected with the Haemoproteus lineage TURDUS2 (prevalence 16.67%), a common parasite of thrushes (Turdidae). Two individuals of C. circumscriptus contained Haemoproteus lineages (prevalence 2.78%); these were the lineage HAWF2 (previously reported from Coccothraustes coccothraustes) and a new lineage CULCIR1 not previously reported in the literature.

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“…Recently several RT-PCR have been also developed for the identification of Culicoides species (Gomulski et al, 2006;Mathieu et al, 2007;Nolan et al, 2007;Cêtre-Sossah et al, 2008).…”

Section: Culicoides Identification and Surveillance Systemsmentioning

confidence: 99%

Scientific review on African Horse Sickness

Boinas¹,

Calistrib

Domingo

et al. 2009

EFSA Supporting Publications

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Rapid diagnostic PCR assays for members of the Culicoides obsoletus and Culicoides pulicaris species complexes, implicated vectors of bluetongue virus in Europe

Cited by 143 publications

References 40 publications

Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom

Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom

A survey of biting midges of the genus Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) in NE Bulgaria, with respect to transmission of avian haemosporidians

Scientific review on African Horse Sickness

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