Rapid Diagnosis of Community-acquired Bacterial Pneumonia

Guzzetta, Philip C.; Toews, GB; Kj, Robertson; Ak, Pierce

doi:10.1164/arrd.1983.128.3.461

Cited by 36 publications

(9 citation statements)

References 24 publications

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“…The etiology would not have been determined if the diagnosis had depended solely on bacterial culture. The advantage of COA over CIE for detection of pneumococcal antigen in respiratory samples, as noted in this study, has been documented previously (26,27). However, studies of antigen detection in serum or urine have yielded conflicting results (2,(28)(29)(30).…”

Section: Discussionsupporting

confidence: 68%

Prospective study on the etiology of community-acquired pneumonia in children and adults in Spain

Ausina

Coll

Sambeat

et al. 1988

Eur. J. Clin. Microbiol. Infect. Dis.

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The cause of primary pneumonia was diagnosed in 157 of 198 children and 165 of 207 adults seen as inpatients or outpatients in a 12-month period. In children Mycoplasma pneumoniae and pneumococcus were identified in 79 and 29 cases respectively. Twenty-nine of 53 cases of viral infection in children were caused by respiratory syncytial virus, two-thirds of the cases occurring in children under three years of age. No children died of pneumonia. In adults pneumococcus was the most common pathogen, accounting for 81 cases. The overall mortality in adults was 7.7%. A high mortality was found in patients with Haemophilus influenzae and other gram-negative bacilli infections, and in elderly patients with pneumococcal pneumonia. Coagglutination was more sensitive than counterimmunoelectrophoresis for the detection of pneumococcal antigen in respiratory samples (p less than 0.001). Counterimmunoelectrophoresis was the only useful technique for detection of pneumococcal antigen in urine specimens, concentration, overnight storage at 4 degrees C and specific staining significantly increasing positivity (p less than 0.001).

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Section: Discussionsupporting

confidence: 68%

Prospective study on the etiology of community-acquired pneumonia in children and adults in Spain

Ausina

Coll

Sambeat

et al. 1988

Eur. J. Clin. Microbiol. Infect. Dis.

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“…Identification of the causative agents plays a key role in the treatment of the infection [4]. An accurate and fast method for the identification may help guide the use of narrow-spectrum antibiotics to reduce the economic burden, relieve the dysbacteriosis, and avoid the emergence of drug-resistant bacteria [5,6].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the microbiota of sputum samples from patients with lower respiratory tract infections

Zhou¹,

Lin²,

Li³

et al. 2010

ABBS

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Sputum is the most common sample collected from patients suffering from lower respiratory tract infections and it is crucial for the bacterial identification of these infections. In this study, we enrolled 101 sputum samples from 101 patients with lower respiratory tract infections. Initially, pyrosequencing of the 16S rDNA V3 hypervariable regions of the bacteria contained in the sputum was utilized as a culture-independent approach for microbiota analysis. For comparison, clinical laboratory tests using a culture-dependent automated bacterial identification system for the same cohort of sputum samples were also done. By pyrosequencing, >70,000 DNA fragments were found and classified into 129 bacterial genera after being analyzed by the Ribosomal Database Project (RDP) process. Most sequences belonged to several predominant genera, such as Streptococcus and Staphylococcus, indicating that these genera play an important role in lower respiratory tract infections. In addition, some sequences belonging to potential causative agents, such as Mycoplasma, Haemophilus, and Moraxella, were also found, but these sequences were not found by clinical laboratory tests. For the nine genera detected by both methods, the methods' sensitivities were compared and the results showed that pyrosequencing was more sensitive, except for Klebsiella and Mycobacterium. Significantly, this method revealed much more complicated bacterial communities and it showed a promising ability for the detection of bacteria.

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“…The detection of PCA in serum and cerebrospinal fluid is not as sensitive as in sputum [1,4,5,7]. The detection of PCA in serum and cerebrospinal fluid is not as sensitive as in sputum [1,4,5,7].…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of pneumococcal antigens in sputa in patients with community-acquired pneumonia by coagglutination

Zhang

Deng

Yao-qing

et al. 1988

Med Microbiol Immunol

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This paper introduces the clinical use of coagglutination as a method for the rapid detection of pneumococcal antigens in patients with community-acquired pneumonia and compares it with the sputum Gram stain and culture methods. Among 105 patients, 50 (48%) were diagnosed as having pneumococcal pneumonia by at least one of the three methods. Of 95 sputa tested, 44 (46%) were found to be positive by the coagulation test, 26 (27%) by Gram staining and only 16 (17%) by the bacterial culture method. The rate of detection of pneumococcal antigens was thus greater with coagulation than with either of the other two methods. The differences were very significant (both P less than 0.01). Our study indicates that the advantages of coagglutination over the traditional bacteriological methods are its speed, sensitivity, convenience and also its relative independence of antibiotic therapy. It thus provides a new dimension in the aetiological diagnosis of pneumococcal pneumonia.

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Rapid Diagnosis of Community-acquired Bacterial Pneumonia

Cited by 36 publications

References 24 publications

Prospective study on the etiology of community-acquired pneumonia in children and adults in Spain

Prospective study on the etiology of community-acquired pneumonia in children and adults in Spain

Analysis of the microbiota of sputum samples from patients with lower respiratory tract infections

Rapid detection of pneumococcal antigens in sputa in patients with community-acquired pneumonia by coagglutination

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