Rapid Development and Characterization of Chromosome Specific Translocation Line of Thinopyrum elongatum with Improved Dough Strength

Kumar, Aman; Kaur, Navneet; Chunduri, Venkatesh; Sharma, Saloni; Misser, Swati; Kumar, Ashish; Tsujimoto, Hisashi; Dou, Quanwen; Gupta, Raj Kumar

doi:10.3389/fpls.2017.01593

Cited by 12 publications

(3 citation statements)

References 57 publications

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“…Progeny lines harboring Th. elongatum chromosomes (segments) incorporated into the wheat genome were obtained as lines with chromosomal additions, substitutions, or translocations [7, 11–14]. However, these introgressions mainly involved the diploid Th.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

Zhang

Liu

et al. 2019

BMC Genomics

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BackgroundTetraploid Thinopyrum elongatum (2n = 4x = 28) is a promising source of useful genes, including those related to adaptability and resistance to diverse biotic (Fusarium head blight, rust, powdery mildew, and yellow dwarf virus) and abiotic (cold, drought, and salt) stresses. However, gene transfer rates are low for this species and relatively few species-specific molecular markers are available.ResultsThe wheat-tetraploid Th. elongatum line K17–841-1 derived from a cross between a hexaploid Trititrigia and Sichuan wheat cultivars was characterized based on sequential genomic and fluorescence in situ hybridizations and simple sequence repeat markers. We revealed that K17–841-1 is a 1E (1D) chromosomal substitution line that is highly resistant to stripe rust pathogen strains prevalent in China. By comparing the sequences generated during genotyping-by-sequencing (GBS), we obtained 597 specific fragments on the 1E chromosome of tetraploid Th. elongatum. A total of 235 primers were designed and 165 new Th. elongatum-specific markers were developed, with an efficiency of up to 70%. Marker validation analyses indicated that 25 specific markers can discriminate between the tetraploid Th. elongatum chromosomes and the chromosomes of other wheat-related species. An evaluation of the utility of these markers in a F2 breeding population suggested these markers are linked to the stripe rust resistance gene on chromosome 1E. Furthermore, 28 markers are unique to diploid Th. elongatum, tetraploid Th. elongatum, or decaploid Thinopyrum ponticum, which carry the E genome. Finally, 48 and 74 markers revealed polymorphisms between Thinopyrum E-genome- containing species and Thinopyrum bessarabicum (Eb) and Pseudoroegneria libanotica (St), respectively.ConclusionsThis new substitution line provide appropriate bridge–breeding–materials for alien gene introgression to improve wheat stripe rust resistance. The markers developed using GBS technology in this study may be useful for the high-throughput and accurate detection of tetraploid Th. elongatum DNA in diverse materials. They may also be relevant for investigating the genetic differences and phylogenetic relationships among E, Eb, St, and other closely-related genomes and for further characterizing these complex species.

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Section: Introductionmentioning

confidence: 99%

Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

Zhang

Liu

et al. 2019

BMC Genomics

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“…Tritici pathotypes. Kumar et al (2017) selected a translocation line with improved dough strength from among wheat-Thinopyrum elongatum derivatives.…”

Section: Introdutionmentioning

confidence: 99%

Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat

Chen

Zhang

et al. 2018

Journal of Integrative Agriculture

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Agropyron cristatum, a wild relative of common wheat (Triticum aestivum L.), provides many desirable genetic resources for wheat improvement, such as tolerance to cold, drought, and disease. To transfer and utilize these desirable genes, in this study, two wheat-A. cristatum derivatives II-13 and II-23 were identified and analyzed. We found that the number of root tip cell chromosomes was 44 in both II-13 and II-23, but there were four and six P genome chromosomes in II-13 and II-23, respectively, based on genomic in situ hybridization (GISH). The chromosome configurations of II-13 and II-23 were both 2n=22II by the meiotic analysis of pollen mother cells (PMCs) at metaphase I, indicating that there were two and three pairs of P chromosomes in II-13 and II-23, respectively. Notably, wheat chromosome 7D was absent in derivative line II-13 while II-23 lacked chromosomes 4B and 7A based on SSR analysis combining fluorescence in situ hybridization (FISH) analysis with pAs1 and pSc119.2 as probes. Chromosomes 2P and 7P were detected in both II-13 and II-23. Another pair of P genome chromosomes in II-23 was determined to be 4P based on expressed-sequences tags-sequence tagged sites (EST-STS) markers specific to A. cristatum and FISH with probes pAcTRT1 and pAcpCR2. Overall, these results suggest that II-13 was a 7P (7D) substitution line with one pair of additional 2P chromosomes and II-23 was a multiple 4P (4B), 7P (7A) substitution line with one pair of additional 2P chromosomes. Moreover, we obtained six alien disomic addition lines and five alien disomic substitution lines by backcrossing. These new materials will allow desirable genes from A. cristatum to be used in common wheat.

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“…It is envisaged that these might influence good chapatti rollability that was also found to better in this line. Contrasting reports of the positive and negative influence of SDSS on chapatti quality have been reported [ 22 , 49 , 50 ]. Higher dough extensibility has been related to easy sheeting/rolling of chapatti dough [ 22 , 51 ] and good chapatti quality.…”

Section: Discussionmentioning

confidence: 94%

A null allele of granule bound starch synthase (Wx-B1) may be one of the major genes controlling chapatti softness

2021

Self Cite

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Chapatti (unleavened flatbread) is a staple food in northern India and neighboring countries but the genetics behind its processing quality are poorly understood. To understand the genes determining chapatti quality, differentially expressed genes were selected from microarray data of contrasting chapatti cultivars. From the gene and trait association studies, a null allele of granule bound starch synthase (GBSS; Wx-B1) was found to be associated with low amylose content and good chapatti quality. For validation, near-isogenic lines (NILs) of this allele were created by marker assisted backcross (MAB) breeding. Background screening indicated 88.2 to 96.7% background recovery in 16 selected BC3F5 NILs. Processing quality and sensory evaluation of selected NILs indicated improvement in chapatti making quality. Traits that showed improvement were mouthfeel, tearing strength and softness indicating that the Wx-B1 may be one of the major genes controlling chapatti softness.

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Rapid Development and Characterization of Chromosome Specific Translocation Line of Thinopyrum elongatum with Improved Dough Strength

Cited by 12 publications

References 57 publications

Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat

A null allele of granule bound starch synthase (Wx-B1) may be one of the major genes controlling chapatti softness

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