Rapid determination of vitamin B2 and B12 in human urine by isocratic liquid chromatography

Mandal, Santi M.; Mandal, Mahitosh; Ghosh, Ananta K.; Dey, Satyahari

doi:10.1016/j.aca.2009.03.009

Cited by 30 publications

(14 citation statements)

References 31 publications

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“…The concentration of urinary RF vary greatly depending on dietary intake, nutritional supplement use, health and physical condition. The ranges of RF urinary previous reported varied from 0.10 to 7.80 mg L -1 [8,9]. As it can be observed in Table 2 the prediction results obtained for the three algorithms in the studied urine samples are in agreement with the values previously mentioned.…”

Section: Urine Samplessupporting

confidence: 87%

“…When RF intake is low, excretion is proportional to the intake [3]. The most widely used analytical methods for the quantification of RF in human urine samples are liquid chromatography (LC)-fluorescence detection (FD) [8], LC-tandem mass spectrometry (MS/MS) [9,10], fluorescence with flow-injection analysis [11], capillary electrophoresis (CE) [12,13], voltammetry [14] and chemiluminescence [15]. These methods require rigorous extraction steps, the use of significant amounts of organic solvents and long analysis time.…”

Section: Introductionmentioning

confidence: 99%

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Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Privitera

Lozano

2017

Microchemical Journal

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A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation-emission fluorescence matrix (EEFM) data in standard addition mode and second-order chemometric analysis. The method is simple, fast and eco-friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial leastsquares/residual bilinearization (U-PLS/RBL) and multidimensional partial leastsquares/residual bilinearization (N-PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.

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Section: Urine Samplessupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Privitera

Lozano

2017

Microchemical Journal

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show abstract

“…1b) is one of the most important biological molecules and an essential nutritional compound in the human diet. Earlier methods used for its determination involved voltammetry (12)(13)(14)(15), chromatographic separation (16,17), spectrophotometry (18) and fluorometric analysis (19). Of these methods, some are complex to operate and some can not meet trace analysis requirements.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence quenching studies on the interaction of riboflavin with tryptophan and its analytical application

Liu

Wang

et al. 2012

Luminescence

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The ineraction between riboflavin (RBF) and tryptophan (Trp) was investigated using fluorescence spectroscopy and UV-vis absorption spectroscopy under physiological conditions. The fluorescence of Trp was quenched by RBF via dynamic quenching, which was analyzed using the Stern-Volmer relation. The value of the Forster distance R0 (2.31 nm) was obtained according to the Forster's theory of nonradiative energy transfer. Under physiological conditions, a linear relationship could be established between the quenched fluorescence intensity of Trp and the concentration of RBF in the range of 5.8 × 10(-7) -2.0 × 10(-5) mol/L. The detection limit was 1.8 × 10(-7) mol/L. The method was successfully applied to determine riboflavin concentrations in pharmaceutical samples.

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“…Analytical methods utilizing high performance liquid chromatography (HPLC)-fluorescence spectroscopy [3][4][5][6][7], HPLC-photo diode array detection [8], HPLC-matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (HPLC-MALDI-TOF) [9], capillary electrophoresis (CE) light emitting diode (LED) fluorescence spectroscopy [10], and CE laser induced fluorescence (LIF) spectroscopy [11,12], have traditionally been used for the determination of riboflavin.…”

Section: Introductionmentioning

confidence: 99%

Quantification of riboflavin in human urine using high performance liquid chromatography–tandem mass spectrometry

Bishop

Fernández

Whitehead

et al. 2011

Journal of Chromatography B

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Rapid determination of vitamin B2 and B12 in human urine by isocratic liquid chromatography

Cited by 30 publications

References 31 publications

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Fluorescence quenching studies on the interaction of riboflavin with tryptophan and its analytical application

Quantification of riboflavin in human urine using high performance liquid chromatography–tandem mass spectrometry

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