Quantification of riboflavin in human urine using high performance liquid chromatography–tandem mass spectrometry

Bishop, Amanda M.; Fernández, Carolina; Whitehead, Ralph D.; Morales-A, Pilar; Barr, Dana Boyd; Wilder, Lynn; Baker, Samuel E.

doi:10.1016/j.jchromb.2011.04.032

Cited by 12 publications

(8 citation statements)

References 16 publications

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“…It is well known that flavins degrade when exposed to light [39,40]. However, this happens only when flavins are present in free (unbound) state in solution [34,41].…”

Section: Effect Of Light-mentioning

confidence: 99%

Flavin homeostasis in the mouse retina during aging and degeneration

Sinha

Makia

et al. 2018

The Journal of Nutritional Biochemistry

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Involvement of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) in cellular homeostasis has been well established for tissues other than the retina. Here, we present an optimized method to effectively extract and quantify FAD and FMN from a single neural retina and its corresponding retinal pigment epithelium (RPE). Optimizations led to detection efficiency of 0.1 pmol for FAD and FMN while 0.01 pmol for riboflavin. Interestingly, levels of FAD and FMN in the RPE were found to be 1.7 and 12.5 folds higher than their levels in the retina, respectively. Both FAD and FMN levels in the RPE and retina gradually decline with age and preceded the age-dependent drop in the functional competence of the retina as measured by electroretinography. Further, quantifications of retinal levels of FAD and FMN in different mouse models of retinal degeneration revealed differential metabolic requirements of these two factors in relation to the rate and degree of photoreceptor degeneration. We also found 2 fold reductions in retinal levels of FAD and FMN in two mouse models of diabetic retinopathy. Altogether, our results suggest that retinal levels of FAD and FMN can be used as potential markers to determine state of health of the retina in general and more specifically the photoreceptors.

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“…It is well known that flavins degrade when exposed to light [39,40]. However, this happens only when flavins are present in free (unbound) state in solution [34,41].…”

Section: Effect Of Light-mentioning

confidence: 99%

Flavin homeostasis in the mouse retina during aging and degeneration

Sinha

Makia

et al. 2018

The Journal of Nutritional Biochemistry

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“…RF is a light sensitive vitamin, so a pool of urine samples was placed in a clear glass bottle and exposed to white light of a lamp for 120 hours [8,10]. This sample was analyzed to confirm that the concentration of RF was not detected by the proposed method.…”

Section: Validation and Test Samplesmentioning

confidence: 99%

“…When RF intake is low, excretion is proportional to the intake [3]. The most widely used analytical methods for the quantification of RF in human urine samples are liquid chromatography (LC)-fluorescence detection (FD) [8], LC-tandem mass spectrometry (MS/MS) [9,10], fluorescence with flow-injection analysis [11], capillary electrophoresis (CE) [12,13], voltammetry [14] and chemiluminescence [15]. These methods require rigorous extraction steps, the use of significant amounts of organic solvents and long analysis time.…”

Section: Introductionmentioning

confidence: 99%

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Privitera

Lozano

2017

Microchemical Journal

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A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation-emission fluorescence matrix (EEFM) data in standard addition mode and second-order chemometric analysis. The method is simple, fast and eco-friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial leastsquares/residual bilinearization (U-PLS/RBL) and multidimensional partial leastsquares/residual bilinearization (N-PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.

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“…Hence, the sensitive and selective detection of riboflavin is of great importance. At present, various analytical methods [34][35][36][37] including high-performance liquid chromatography, surface plasmon resonance, mass spectrometry, fluorescence, and spectrophotometry, have been established for the detection of this analyte. However, most of the analytical techniques require complicated instrumentation, have relatively high cost, or suffer from poor sensitivity and low selectivity.…”

Section: Introductionmentioning

confidence: 99%