Rapid determination of solanum glycoalkaloids by thin-layer chromatographic scanning

Ferreira, Fernando; Moyna, Patrick; Soulé, Silvia; Vázquez, Álvaro

doi:10.1016/0021-9673(93)83200-c

Cited by 31 publications

(15 citation statements)

References 9 publications

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“…Various analytical techniques are used for the determination of steroidal alkaloids and glycoalkaloids, including mass spectrometry (MS) [13], isotachophoresis [14], thinlayer chromatography [15], colorimetric methods [16], gas chromatography [17,18], countercurrent chromatography [19], high-pressure liquid chromatography (HPLC) [20,21], and immunoassays [22,23]. HPLC methods are more frequently used even though detection by UV absorbance is problematic because GAs lack a strong UV-visible chromophore.…”

Section: Introductionmentioning

confidence: 99%

Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry

Bianco

Schmitt‐Kopplin²,

Benedetto³

et al. 2002

Electrophoresis

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Glycoalkaloids are naturally occurring nitrogen-containing compounds present in many species of the family Solanaceae, including cultivated and wild potatoes (Solanum spp.), tomatoes (Lycopersicon spp.), etc. These compounds have pharmacological and toxicological effects on humans due to their significant anticholinesterase activity and disruption of cell membranes. Herein is reported the development of a capillary electrophoresis (CE) method using nonaqueous (NA) separation solutions in combination with ion trap mass spectrometry (MS and MS/MS) detection for the identification and quantification of glycoalkaloids and their relative aglycones. A mixture 90:10 v/v of MeCN-MeOH containing 50 mM ammonium acetate and 1.2 M acetic acid (applied voltage of 25.5 kV) was selected as a good compromise for the separation and detection of these compounds. The electrospray MS measurements were carried out in the positive ionization mode using a coaxial sheath liquid, methanol-water (1:1) with 1% of acetic acid at a flow rate of 2.5 microL/min. Under optimized experimental conditions, the predominant ion was the protonated molecular ion ([M+H](+)) of solanidine (m/z = 398), tomatidine (m/z = 416), chaconine (m/z = 852), solanine (m/z = 868), and tomatine (m/z = 1034). MS/MS experiments were carried out systematically by changing the relative collisional energy and monitoring the intensities of the fragment ions that were not high enough to allow better quantification than with the mother ions. The method was used for analyzing glycoalkaloids in potato extracts.

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Section: Introductionmentioning

confidence: 99%

Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry

Bianco

Schmitt‐Kopplin²,

Benedetto³

et al. 2002

Electrophoresis

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“…The purity of the products was determined by TLC (Ferreira et al, 1993) and by measurement of the optical rotation of the products and comparison with literature data (Kuhn & L0w, 1954).…”

Section: Resultsmentioning

confidence: 99%

“…In each step the potato glycoalkaloids were quantified following the method described by Ferreira et al (1993).…”

Section: Methodsmentioning

confidence: 99%

Preparative isolation ofSolanum tuberosum L. glycoalkaloids by MPLC

Soulé¹,

Vázquez²,

González³

et al. 1997

Potato Res

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SummaryA new, efficient and economic method employing Medium Pressure Liquid Chromatography (MPLC) for the isolation of the two major Solanunl tuberosum L. glycoalkaloids (c~-solanine and ct-chaconine) is described. Potato peelings are homogenised with 5% acetic acid, the glycoalkaloids purified by filtration through an XAD-2 column and then by precipitation from the aqueous solution. The resulting glycoalkaloid fraction was purified by MPLC using a Silica Gel column and a CHCI3:MeOH:2% NH4OH mixture (70:30:5) as mobile phase to yield pure et-chaconine and a-solanine. This methodology can be used to obtain glycoalkaloids for enthomology and toxicological research where large amounts of these compounds are required.

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“…Data treatment. The values for each class of compound were graphed by means of a linear correlation (11)(12)(13)(14). The concentrations in the problems were calculated, averaged, and expressed as the molar ratio of (lipophilic compound)/(DPPC + lipophilic compound).…”

Section: Methodsmentioning

confidence: 99%

“…Such a method only requires a TLC separation of the components, avoiding most of the possible interferences while having very high sensitivity. We have previously applied direct TLC-densitometric quantification to a number of natural products, e.g., cuticular waxes (11), carbohydrates (12), steroidal alkaloids (13), and terpenes in essential oils (14). The measured refraction values are linearly dependant on the applied concentration, and the unknown concentration can be calculated from the standard curves.…”

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confidence: 99%

Determination of the phospholipid/lipophilic compounds ratio in liposomes by thin‐layer chromatography scanning densitometry

Rodrı́guez¹,

Cesio²,

Heinzen³

et al. 2000

Lipids

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The determination of the ratio of phospholipid/lipophilic compounds in liposomes was achieved after thin-layer chromatography (TLC) by measuring the spot intensities of dipalmitoyl phosphatidylcholine and the lipophilic compound. The liposome components under study were separated on one TLC plate, developed in two steps, and detected after charring the plate with specific visualization reagents. The method shows good reproducibility and provides a simple way to quantify the level of lipophilic compound incorporated in the liposome bilayer.

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Rapid determination of solanum glycoalkaloids by thin-layer chromatographic scanning

Cited by 31 publications

References 9 publications

Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry

Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization-ion trap mass spectrometry

Preparative isolation ofSolanum tuberosum L. glycoalkaloids by MPLC

Determination of the phospholipid/lipophilic compounds ratio in liposomes by thin‐layer chromatography scanning densitometry

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