Rapid determination of metformin in human plasma using ion-pair HPLC

Zarghi, Afshin; Foroutan, Seyed Mohsen; Shafaati, Alireza; Khoddam, Arash

doi:10.1016/s0731-7085(02)00608-8

Cited by 98 publications

(63 citation statements)

References 9 publications

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“…Type 2 diabetes is progressive in nature and glycemic control become difficult in most patients, requiring either increased Metformin dosage or supplementary (add-on) therapy. 6 A survey of the literature revealed some HPLC and LC-MS/MS methods for analysis of MET alone [7][8][9][10][11][12][13] and in combination with other drugs. However, quite a few HPLC methods have been reported for simultaneous determination of MET with SIT [14][15][16][17][18][19][20][21][22] or VLD [23][24][25][26][27][28] or LIN 29,30 in formulations, but, no simple and rapid method is yet established for simultaneous determination of Metformin, Sitagliptin, Vildagliptin and Linagliptin using single analytical procedure.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Attimarad¹,

Nagaraja²,

Aldhubaib³

et al. 2014

IJPER

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Objective: A simple, rapid and validated liquid chromatographic method has been developed for the simultaneous determination of three novel Gliptins namely Vildagliptin (VLD), Sitagliptin (SIT) and Linagliptin (LIN) in their binary mixture with Metformin (MET). Methodology: The separation was performed on fast monolithic column using isocratic, mobile phase consisting of mixture of sodium dihydrogen phosphate, Sodium dedosyl sulphate and acetonitrile. The flow rate was 2.5 mL/min and UV detection for MET, VLD and SIT was conducted at 208 nm, whereas, that of MET and LIN was at 228 nm. The incipiently developed method was subjected for validation according to ICH guidelines. Results: The calibration curves of analytes showed good correlation (r2>0.999) over a concentration range 10-100 μg/mL and 50-400 μg/mL for Metformin, 1-10 μg/mL for Vildagliptin & Sitagliptin and 0.25-2.0 μg/mL for Linigliptin. Conclusion: All three tablet formulations were assayed with accuracy and precision and without interference from excipients. The method is also stability indicating with respect to Linagliptin.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Attimarad¹,

Nagaraja²,

Aldhubaib³

et al. 2014

IJPER

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“…MET To the best of our knowledge no method is reported in literature for simultaneous determination of MET and REPA by high performance liquid chromatography (HPLC). Several HPLC methods have been reported in the literature for quantitative determination of MET alone [4] or in combination with other drugs in tablets [5][6][7], human serum and other biological fluids [8][9]. Similarly, a few HPLC methods have been reported for the quantitative determination of REPA in tablets alone or in combination with other drugs [10][11][12], and in human serum and other biological fluids [13,14].…”

Section: Introductionmentioning

confidence: 99%

Validated stability-indicating RP-HPLC UV method for simultaneous determination of metformin and repaglinide

Joshi

Nahire

Shastri

et al. 2012

Acta Chromatographica

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Summary.A simple, rapid, precise, and accurate, stability-indicating reversed phase high performance liquid chromatographic method was developed and validated for simultaneous determination of metformin HCl and repaglinide. The chromatographic separation was achieved on YMC Pack AM ODS (5 μm, 250 mm length × 4.6 mm i.d.) column at a detector wavelength of 210 nm, using an isocratic mobile phase consisting of methanol and 10 mM potassium dihydrogen phosphate buffer (pH 2.5) in a ratio of 70:30 v/v at a flow rate of 1 mL min −1 . The retention times for metformin and repaglinide were found to be 2.6 and 11.3 min, respectively. The drugs were exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. Validation of the method was carried out as per International Conference on Harmonization (ICH) guidelines. Linearity was established for metformin and repaglinide in the range of 5-200 μg mL −1 and 1-200 μg mL −1 , respectively. The limits of detection were 0.3 μg mL −1 and 0.13 μg mL −1 for metformin and repaglinide, respectively. The method was found to be specific and stability-indicating as no interfering peaks of degradants and excipients were observed. The proposed method is hence suitable for application in quality-control laboratories for quantitative analysis of both the drugs individually and in combination, since it is simple and rapid with good accuracy and precision.

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“…[1][2][3] Published methods for the determination of MET are based on different techniques like capillary electrophoresis, 4,5 NMR spectrometry, 6 potentiometry, [7][8][9] spectrofluorimetry and spectrophotometry, [9][10][11][12][13][14][15][16][17][18][19][20] conductometry, 21,22 voltammetry, [23][24][25][26][27][28][29] IR spectrometry 30 and chromatography. [31][32][33][34][35][36][37][38][39] Although many of the reported methods are accurate and sensitive, they require the use of sophisticated equipment and expensive reagents. Some are cumbersome, requiring prolonged sample pretreatment, strict control of p-H and long reaction times.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Assay of Metformin Hydrochloride Using Pyrogallol Modified Carbon Paste Electrode

Wm²,

Am³

2015

ACSi

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The electrooxidative behavior and determination of metformin hydrochloride, anti-hyperglycemic drug, on a pyrogallol modified carbon paste electrode were investigated using cyclic voltammetry and differential pulse voltammetry. Metformin hydrochloride shows an irreversible oxidation behavior over a wide interval of pH (Britton-Robinson buffers, pH 2-9). The peak current varied linearly in the range comprised between 8.0 × 10 -7 and 6.0 × 10 -6 mol/L with detection limit of 6.63 × 10 -8 mol/L and limit of quantification of 2.21 × 10 -7 mol/L. The method was proposed for the determination of metformin hydrochloride in dosage forms and urine.

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Rapid determination of metformin in human plasma using ion-pair HPLC

Cited by 98 publications

References 9 publications

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Simultaneous Determination of Metformin and Three Gliptins in Pharmaceutical Formulations Using RP HPLC: Application to Stability Studies on Linagliptin Tablet Formulation

Validated stability-indicating RP-HPLC UV method for simultaneous determination of metformin and repaglinide

Voltammetric Assay of Metformin Hydrochloride Using Pyrogallol Modified Carbon Paste Electrode

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