Rapid determination of losartan and losartan acid in human plasma by multiplexed LC–MS/MS

Abstract

A rapid LC-MS/MS method has been developed and validated for the determination of losartan (LOS) and its metabolite losartan acid (LA) (EXP-3174) in human plasma using multiplexing technique (two HPLC units connected to one MS/MS). LOS and LA were extracted from human plasma by SPE technique using Oasis HLB cartridge without evaporation and reconstitution steps. Hydroflumethiazide (HFTZ) was used as an internal standard (IS). The analytes were separated on Zorbax SB C-18 column. The mass transition [M-H] ions used for detection were m/z 421.0 --> 127.0 for LOS, m/z 435.0 --> 157.0 for LA, and m/z 330.0 --> 239.0 for HFTZ. The proposed method was validated over the concentration range of 2.5-2000 ng/mL for LOS and 5.0-3000 ng/mL for LA with correlation coefficient > or = 0.9993. The overall recoveries for LOS, LA, and IS were 96.53, 99.86, and 94.16%, respectively. Total MS run time was 2.0 min/sample. The validated method has been successfully used to analyze human plasma samples for applications in 100 mg fasted and fed pharmacokinetic studies.

Losartan

“…Shah et al [46] developed a rapid LC-MS/MS method for the determination of losartan (LOS) and its metabolite LA (EXP3174) in human plasma using multiplexing technique (two HPLC units connected to one MS/MS). LOS and LA were extracted from human plasma by SPE technique using Oasis HLB cartridge without evaporation and reconstitution steps.…”

Analysis of Sartans: A Review

“…Tables present some of the recently reported HPLC methods for the analysis of sartans in biological fluids (plasma and urine) …”

Conductometric Determination of Losartan Potassium Using Phosphotungstic Acid, Bromocresol Purple, Mercury (Ii) Chloride, and Cupric Chloride as Reagents

“…Furthermore, fluorescence spectroscopic method has been reported for the determination of LK [7]. Furthermore, different chromatographic methods such as HPLC with UV detection [8,9], mass detection [10], thin-layer chromatography [11], and capillary electrophoresis [12] have been published for determination of LK. Electrochemical methods have also been reported including conductometric methods [13,14] and potentiometric method [15].…”