Rapid determination of clenbuterol residues in urine by high-performance liquid chromatography with on-line automated sample processing using immunoaffinity chromatography

Haasnoot, W.; Ploum, M.E.; Paulussen, R. J. A.; Schilt, R.; Huf, F.A.

doi:10.1016/0021-9673(90)85161-n

Cited by 55 publications

(16 citation statements)

References 13 publications

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“…Oestrogen steroids have also been determined in spiked urine samples using automated columnswitching method with a total analysis time of 45 min and capacity to analyse 30 samples per day [118]. Other use of IACs have included natural hormones in calf urine [100], and tissues [101], clenbuterol in urine [102] and trenbolones in calf and cattle urine [121]. A GC/MS method with IAC clean-up was developed for the determination of derivatised zeranol and related compounds, taleranol, zearalanone, and ␣-zearalenol in bovine muscle, using methanol extraction and IAC columns containing monoclonal antibodies raised against zeranol coupled to CNBr-activated Sepharose 4B [122] On-line coupling of IAC with automated with column switching has been used for the determination of four fluoroquinolone antibiotics in chicken liver [105].…”

Section: Veterinary Drugsmentioning

confidence: 99%

“…The analytes were captured on 100 l home-made IAC, eluted to a reverse-phase pre-column, switched to a reverse phase analytical column and finally analysed by ESI LC/MS [99]. Other on-line columns switching systems have been developed for chloramphenicol in milk and muscle tissue [70], natural hormones in calf urine [100], calf tissue [101], clenbuterol in urine [102], trenbolones in calf and cattle urine [101], and fluoroquinolones in serum [103], milk [104] and chicken liver [105].…”

Section: Automation Of Iacmentioning

confidence: 99%

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Immunoaffinity column clean-up techniques in food analysis: A review

Şenyuva

Gilbert

2010

Journal of Chromatography B

144

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Section: Veterinary Drugsmentioning

confidence: 99%

Section: Automation Of Iacmentioning

confidence: 99%

Immunoaffinity column clean-up techniques in food analysis: A review

Şenyuva

Gilbert

2010

Journal of Chromatography B

144

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“…Due to the necessity to detect and monitor β-agonists, several methods have been developed for determination of these compounds, including gas chromatography with mass spectrometric detection (GC-MS) [17,18] and high performance liquid chromatographic (HPLC) method with UV [19], electrochemical [20] and MS [21,22] detections. Some reported methods are based on capillary electrophoresis (CE) with MS [23] and amperometry [24] detection.…”

Section: Introductionmentioning

confidence: 99%

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Karuwan

Mantim

Chaisuwan

et al. 2006

Sensors

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This work presents the construction and application of boron-doped diamond (BDD) thin film electrode as sensor for the determination of three β-agonists, viz. salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inert material, BDD film however shows fouling in detection of these compounds using fixedpotential mode amperometry. A suitable waveform for pulsed amperometric detection (PAD) was developed and used to determine the agonist compounds. It was seen that the developed PAD significantly refreshed the BDD surface for long-term detection in flow injection analysis. Linear working ranges were 0.5-100 µM, 1.0-100 µM and 0.5-50 µM for salbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system was applied to successfully determine salbutamol and terbutaline in commercial pharmaceutical products. The methods were validated with a capillary electrophoresis method.

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“…Methods with UV detection were performed in rat brain (Botterblom et al, 1993), retina (González Gigosos et al, 1996), calf urine (Haasnoot et al, 1990) and beef liver and muscle tissue (Lawrence and Ménard, 1997). The retina and liver are known to contain high concentrations of clenbuterol.…”

Section: Resultsmentioning

confidence: 99%

Quantitative determination of clenbuterol enantiomers in human plasma by high-performance liquid chromatography using the macrocyclic antibiotic chiral stationary phase teicoplanin1

Aboul‐Enein

Serignese

1999

Biomed. Chromatogr.

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We report a method for the high‐performance liquid chromatographic (HPLC) chiral separation of racemic clenbuterol in human plasma. Human plasma was spiked with stock solutions of clenbuterol hydrochloride and practolol as the internal standard. Following a liquid–liquid extraction procedure with 10% (+/−)‐2‐butanol/isopropyl ether under alkaline conditions, the dried samples were reconstituted in methanol and chromatographed using a macrocyclic antibiotic chiral stationary phase (CSP) known as Chirobiotic T™ (teicoplanin). The mobile phase composition was methanol:acetonitrile (70:30, v/v), containing 0.3% (v/v) acetic acid and 0.2% (v/v) triethylamine. The resulting chromatogram achieved baseline separation for the clenbuterol enantiomers. Calibration curves (peak area ratio vs plasma concentration, n = 10) were constructed for the (−)‐R‐and (+)‐S‐clenbuterol enantiomers with a plasma concentration range of 0.25–10 µM. The correlation coefficient (r) range was 0.99988–0.99999 (mean = 0.99999). The lowest concentration measured was 0.25 µM. Inter‐ and intra‐assay variation was determined for the lowest, medium and highest plasma concentration (0.25, 2 and 10 µM) by calculating the analytical recoveries with a range of 96–104%. The percentage recoveries for the clenbuterol enantiomers were 88.4–102% over the concentration range used. Detailed methodology is presented. Copyright © 1999 John Wiley & Sons, Ltd.

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Rapid determination of clenbuterol residues in urine by high-performance liquid chromatography with on-line automated sample processing using immunoaffinity chromatography

Cited by 55 publications

References 13 publications

Immunoaffinity column clean-up techniques in food analysis: A review

Immunoaffinity column clean-up techniques in food analysis: A review

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Quantitative determination of clenbuterol enantiomers in human plasma by high-performance liquid chromatography using the macrocyclic antibiotic chiral stationary phase teicoplanin1

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