Rapid determination of canagliflozin in rat plasma by UHPLC–MS/MS using negative ionization mode to avoid adduct-ions formation

Analysis of Canagliflozin, Review

“…[24] Another validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitative analysis of canagliflozin in a lower volume of rat plasma (0.1 mL) was established and applied to a pharmacokinetic study in rats. were obtained.…”

A validated LC‐MS/MS method for the determination of canagliflozin, a sodium–glucose co‐transporter 2 (SGLT‐2) inhibitor, in a lower volume of rat plasma: application to pharmacokinetic studies in rats

“…In previous reported studies, both positive and negative ionization were adapted for analyzing canagliflozin in biological samples; ammonium adduct ions [M + NH 4 ] + (m/z = 462.1) in positive ionization were adapted for the determination of canagliflozin levels in plasma in clinical trials using an API 4000 equipped with turbo ion spray interface (AB Sciex, Framingham, MA, USA; Devineni et al, 2013Devineni et al, , 2014Devineni et al, , 2015Inagaki et al, 2014;Murphy et al, 2015). Single abundant product ions [M-H] -(m/ z = 443.0) in negative ionization were adapted for analyzing canagliflozin in rat plasma using the ACQUITY TM UPLC system coupled to a triple-quadruple tandem mass spectrometer (Micromass Quattro micro TM Waters Corp., Milford, MA,USA; Iqbal et al, 2015). In the current study, different mobile phase (containing formic or acetic acid, and ammonium salt) and different ionization mode (positive and negative) were initially evaluated using ESI sources, and the results showed that positive ionization gave more sensitive ions than negative ionization.…”

“…Analytical methods, including ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) (Iqbal et al, 2015) and high-performance liquid chromatography-MS/MS (LC-MS/MS) (Devineni et al, 2013(Devineni et al, , 2014Inagaki et al, 2014;Murphy et al, 2015), have been used for quantitative analysis of canagliflozin in different biological matrices. Iqbal et al (2015) have reported an UHPLC-MS/MS assay for the rapid determination of canagliflozin in rat plasma with a lower limit of quantification (LLOQ) of 3.76 ng/mL for 0.2 mL plasma.…”

“…Iqbal et al (2015) have reported an UHPLC-MS/MS assay for the rapid determination of canagliflozin in rat plasma with a lower limit of quantification (LLOQ) of 3.76 ng/mL for 0.2 mL plasma. However, the UHPLC-MS/MS method reported by Iqbal et al (2015) has the limitation of not being suitable for long-term studies investigating both pharmacokinetics and pharmacodynamics (blood glucose levels) of hypoglycemic agents that require multiple blood sampling, because the method requires 0.5 mL blood from rats and a 0.2 mL plasma sample -a relatively large volume. Moreover, although the UHPLC-MS/MS method provides faster separations, sensitive analysis and lower mobile phase solvent consumption, the instrumentation is expensive and requires highly technical skills; therefore, it is not usually available in most clinical and experimental laboratories.…”

A Validated Lc-MS/MS Method for Pharmacokinetic Study of Canagliflozin in Healthy Rabbits

“…As per the Literature Survey, it is revealed that the Ultraviolet spectroscopy (UV), High-Performance Liquid Chromatography (HPLC) and High-Performance thin layer Chromatography (HPTLC) methods were reported for degradation studies and to estimate the canagliflozin from bulk and Pharmaceutical dosage forms [5][6][7][8][9][10]. Ultra High Performance/liquid Chromatography-Mass Spectroscopy (UHPLC-MS and LC-MS/MS) methods were reported for quantification of the drug in biological fluids like human and rat plasma [11,12]. To best of our knowledge, no published LC-MS/MSbased methods for the pharmacokinetic study of canagliflozin in healthy rabbits.…”