Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by β-cyclodextrin-modified capillary zone electrophoresis

Li, Wei; Gong, Suxuan; Wen, Dawei; Che, Baoquan; Liao, Yiping; Liu, Huwei; Feng, Xuefeng; Shilin, Hu

doi:10.1016/j.chroma.2004.08.008

Cited by 31 publications

(1 citation statement)

References 29 publications

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“…Current approaches for detection and quantitation of AAs in herbal medicine include capillary electrophoresis coupled with UV absorption or electrochemical detection and HPLC coupled with UV absorption or fluorescence detection. [37][38][39][40] According to the Chinese Pharmacopoeia, 6 the HPLC-UV method is used as the standard method to analyse the AA content in herbal medicine; therefore, in this study, we first tested for the presence of AA-I and AA-II in H. cordata samples by HPLC-UV analysis.…”

Section: Lc-uv Analysis Of Aas In H Cordata Samplesmentioning

confidence: 99%

Analysis of aristolochic acids in Houttuynia cordata by liquid chromatography–tandem mass spectrometry

2020

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Houttuynia cordata (H. cordata) is a popular vegetable in Asian countries and is also used extensively as herbal medicine in treating various diseases. H. cordata contains aristolactams, which have a similar Chinese name as aristolochic acids (AAs); hence, an emerging concern in the greater China region has arisen about the potential linkage between H. cordata and aristolochic acid nephropathy (AAN). However, only a single study has tested for the presence of AAs in H. cordata samples, and the analysis was limited by the analytical sensitivity of the method. Thus, further analysis of AAs in H. cordata using analytical method of higher sensitivity is needed to alleviate public anxiety over the use of this popular vegetable. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used to analyze H. cordata samples for the natural existence of aristolochic acid I (AA-I) and aristolochic acid II (AA-II), which are the most carcinogenic and nephrotoxic compounds in the AA family. After evaluating the method performance by fortifying blank samples with three concentrations of AAs, the validated method was applied to identify AA-I and AA-II in both fresh and sun-dried H. cordata samples (n = 20) collected from different cities in China. The LC-MS/MS method achieved method detection limits (MDLs) as low as 2 ng/g of AAs in H. cordata. Analysis of the collected fresh and sun-dried H. cordata samples revealed that AA-I and AA-II either do not exist naturally in H. cordata or exist at concentrations below the MDLs. Therefore, it is not very likely that consumption of H. cordata will result in AAN because AA-I and AA-II, the nephrotoxic and carcinogenic culprits of AAN, are not produced naturally in the plant or are produced at levels that do not pose a risk of AAN.

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Section: Lc-uv Analysis Of Aas In H Cordata Samplesmentioning

confidence: 99%

Analysis of aristolochic acids in Houttuynia cordata by liquid chromatography–tandem mass spectrometry

2020

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Simultaneous determination of iridoid glycosides and flavanoids in Lamionphlomis rotate and its herbal preparation by a simple and rapid capillary zone electrophoresis method

Lü

Chen

et al. 2011

Drug Testing and Analysis

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Iridoid glycosides and flavanoids are two main effective components of Lamiophlomis rotata (Benth.) kudo. However, there is no method for simultaneous analysis of iridoid glycosides and flavanoids in L. rotata and its pharmaceutical preparations. A simple and rapid capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of two iridoid glycosides (8-O-acetylshanzhiside methylester and 8-deoxyshanzhiside) and three flavanoids (apigenin, quercetin and luteolin) in L. rotata. Operational variables, such as the voltage, buffer concentration and pH were optimized, the final optimum separation condition was 10 mM sodium tetraborate-20 mM NaH(2) PO(4) (pH 8.5)-15% (v/v) methanol, 238 nm UV detection, 18 kV applied voltage. The linearity and the recovery of the proposed method were very satisfactory (correlation coefficients were 0.9994-0.9998 and the recoveries were 94.5-108.8% for the analytes) and the method allowed analytes in real samples to be determined within 9 min. The proposed CZE method can be used for quality control of iridoid glycosides and flavanoids in L. rotata and its herbal preparation.

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Analysis of aristolochic acids by CE‐MS with carboxymethyl chitosan‐coated capillary

Liu

et al. 2009

Electrophoresis

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A CE-MS method for rapid determination of aristolochic acid-I and aristolochic acid-II (AA-II) in traditional Chinese medicines and biological samples was described in the present paper. AA-I and AA-II can be baseline separated within 6 min by CE-MS with carboxymethyl-chitosan-coated capillary. CZE conditions including pH, concentration of buffer, applied voltage, and capillary temperature were systematically investigated, and the composition and flow rate of sheath liquid were also optimized for CE-MS. Furthermore, the CE-UV method without any additives in BGE solution was established and compared with the CE-MS method. The results showed that the two methods could achieve satisfactory separation efficiency, repeatability, and linearity, while the LOD was 0.6 microg/mL for CE-UV and 0.05 microg/mL for CE-MS. Compared with the CE-UV method, the sensitivity of CE-MS was significantly improved, in addition to the structure information provided by MS detection at the same time. As an application example, a spiked sample in human serum was analyzed by the CE-MS method, indicating that the new CE-MS method can be applied to analyze AAs in biological samples.

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Rapid determination of aristolochic acid I and II in Aristolochia plants from different regions by β-cyclodextrin-modified capillary zone electrophoresis

Cited by 31 publications

References 29 publications

Analysis of aristolochic acids in Houttuynia cordata by liquid chromatography–tandem mass spectrometry

Analysis of aristolochic acids in Houttuynia cordata by liquid chromatography–tandem mass spectrometry

Simultaneous determination of iridoid glycosides and flavanoids in Lamionphlomis rotate and its herbal preparation by a simple and rapid capillary zone electrophoresis method

Analysis of aristolochic acids by CE‐MS with carboxymethyl chitosan‐coated capillary

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