Rapid determination by high performance liquid chromatography of di‐2‐ethylhexyl phthalate in plasma stored in plastic bags

Abstract

A rapid sensitive technique was developed for the analysis of di-2-ethylhexyl phthalate (DEHP) in plasma stored in plastic bags by using high performance liquid chromatography (HPLC) with UV detection and a Hypersil ODS column. The compound was easily and efficiently extracted with a mixture of sodium hydroxide and acetonitrile, which allowed the deproteinization of plasma samples. The recovery was greater than 95% and the intra- and inter-assay coefficients of variation were better than 6.5%. The results obtained showed that the amount of DEHP accumulated in plasma varied according to different parameters and depended on the storage conditions (time, temperature and shaking) and also on the lipid content of the stored plasma and the sterilization process of the PVC bags.

Migration of diethylhexyl phthalate from PVC bags into intravenous cyclosporine solutions

“…In accordance with our results, Dine et al [18] did not also detected DEHP in water, 0.9% NaCl or 5% glucose stored in plastic bags for 1 year. Otherwise, Arbin andÖstelius [19] quantified mono 2-ethylhexyl phthalate and DEHP in 5% glucose and 0.9% NaCl stored in PVC bags during 15 days to 14 months.…”

Leaching of diethylhexyl phthalate from polyvinyl chloride bags into intravenous etoposide solution

“…Very little or no DEHP is detectable in water, 0·9% sodium chloride or 5% glucose stored in PVC bags for more than 1 year (13). Formulations containing surfactants such as polyoxyethylated castor oil or polysorbate 80 leached DEHP from PVC containers (9).…”

Determination of di(2-ethylhexyl)phthalate and mono(2-ethylhexyl)phthalate in human serum using liquid chromatography-tandem mass spectrometry

“…Patients undergoing medical procedures, such as intravenous therapy, nutritional support, blood transfusion, hemodialysis, cardiopulmonary bypass or extracorporeal membrane oxygenation (EMO) can be exposed to DEHP. Previous studies have shown detectable amounts of DEHP in blood products, in intravenous solutions, and in intravenous fat emulsions stored in PVC bags [1][2][3][4][5]. In animal studies, DEHP and/or MEHP are toxicants to the reproductive and developmental systems [6][7][8][9][10].…”